##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AatII AclI AgeI Aor51HI AscI
AseI AsnI BbvCI BciVI BclI
BsiEI BsiWI BspDI BspLU11I BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoT22I FbaI FseI HindIII MluI
NdeI NotI NruI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RsrII SexAI SfuI SgrAI
SpeI SspI SwaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 3435 GGTACC G^GTACC
AccI 4264 GTCGAC GT^[AC][GT]AC
AccIII 1417 TCCGGA T^CCGGA
AflII 4475 CTTAAG C^TTAAG
Asp718I 3435 GGTACC G^GTACC
AvrII 5769 CCTAGG C^CTAGG
BcgI 5475 GCAGAGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4475 CTTAAG C^TTAAG
BlnI 5769 CCTAGG C^CTAGG
BsaBI 2755 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1417 TCCGGA T^CCGGA
BsmBI 5351 GAGACG CGTCTC(1/5)
BspEI 1417 TCCGGA T^CCGGA
DraI 5491 TTTAAA TTT^AAA
HpaI 6608 GTTAAC GTT^AAC
KpnI 3435 GGTACC GGTAC^C
KspI 6807 CCGCGG CCGC^GG
MamI 2755 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4927 CAATTG C^AATTG
MroI 1417 TCCGGA T^CCGGA
MunI 4927 CAATTG C^AATTG
NheI 1648 GCTAGC G^CTAGC
PaeR7I 3889 CTCGAG C^TCGAG
SacII 6807 CCGCGG CCGC^GG
SalI 4264 GTCGAC G^TCGAC
SbfI 5368 CCTGCAGG CCTGCA^GG
ScaI 3210 AGTACT AGT^ACT
SfiI 1762 GGCCAGCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 3137 TACGTA TAC^GTA
Sse8387I 5368 CCTGCAGG CCTGCA^GG
XbaI 6769 TCTAGA T^CTAGA
XhoI 3889 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 45 enzymes) #
###################################
AcsI 1274 GAATTC [GA]^AATT[CT]
3487 GAATTC
AhdI 2327 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3809 GACCTCAAGTC
Alw44I 6653 GTGCAC G^TGCAC
6663 GTGCAC
AocI 2398 CCTAAGG CC^T[ACGT]AGG
2512 CCTCAGG
ApaI 3168 GGGCCC GGGCC^C
3912 GGGCCC
ApaLI 6653 GTGCAC G^TGCAC
6663 GTGCAC
ApoI 1274 GAATTC [GA]^AATT[CT]
3487 GAATTC
AspEI 2327 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3809 GACCTCAAGTC
AspI 3056 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
4032 GACAGCGTC
AviII 1542 TGCGCA TGC^GCA
6815 TGCGCA
BalI 1770 TGGCCA TGG^CCA
1851 TGGCCA
BamHI 913 GGATCC G^GATCC
2419 GGATCC
BbeI 1236 GGCGCC GGCGC^C
5618 GGCGCC
BsaI 1696 GAGACC GGTCTC(1/5)
6321 GGTCTC
BsaWI 1417 TCCGGA [AT]^CCGG[AT]
2823 ACCGGA
Bsp1407I 4239 TGTACA T^GTACA
5106 TGTACA
BspHI 3321 TCATGA T^CATGA
4146 TCATGA
BsrFI 2168 GCCGGC [GA]^CCGG[CT]
5664 GCCGGC
BsrGI 4239 TGTACA T^GTACA
5106 TGTACA
BstEII 2243 GGTGACC G^GT[ACGT]ACC
3938 GGTTACC
BstPI 2243 GGTGACC G^GT[ACGT]ACC
3938 GGTTACC
Bsu36I 2398 CCTAAGG CC^T[ACGT]AGG
2512 CCTCAGG
Cfr10I 2168 GCCGGC [GA]^CCGG[CT]
5664 GCCGGC
Eam1105I 2327 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3809 GACCTCAAGTC
Eco81I 2398 CCTAAGG CC^T[ACGT]AGG
2512 CCTCAGG
EcoO65I 2243 GGTGACC G^GT[ACGT]ACC
3938 GGTTACC
EcoRI 1274 GAATTC G^AATTC
3487 GAATTC
EcoRV 2309 GATATC GAT^ATC
5429 GATATC
FspI 1542 TGCGCA TGC^GCA
6815 TGCGCA
KasI 1236 GGCGCC G^GCGCC
5618 GGCGCC
MluNI 1770 TGGCCA TGG^CCA
1851 TGGCCA
MscI 1770 TGGCCA TGG^CCA
1851 TGGCCA
NaeI 2168 GCCGGC GCC^GGC
5664 GCCGGC
NarI 1236 GGCGCC GG^CGCC
5618 GGCGCC
NgoMIV 2168 GCCGGC G^CCGGC
5664 GCCGGC
PflFI 3056 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
4032 GACAGCGTC
PshAI 6115 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6830 GACTGGTGTC
RcaI 3321 TCATGA T^CATGA
4146 TCATGA
SapI 901 GAAGAGC GCTCTTC(1/4)
3343 GCTCTTC
SfoI 1236 GGCGCC GGC^GCC
5618 GGCGCC
SmaI 404 CCCGGG CCC^GGG
4391 CCCGGG
SspBI 4239 TGTACA T^GTACA
5106 TGTACA
Tth111I 3056 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
4032 GACAGCGTC
XmaCI 404 CCCGGG C^CCGGG
4391 CCCGGG
XmaI 404 CCCGGG C^CCGGG
4391 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI Asp700I AspHI
AvaI AvaII BanI BanII BbrPI
BbsI BbvI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI