##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AscI AseI AsnI AspEI
AviII AvrII BciVI BfrI BlnI
BseAI BsiWI BspEI BspLU11I Bst1107I
BstZ17I CpoI DraI Eam1105I EcoRI
EcoRV EcoT22I FspI HincII HindII
HpaI MfeI MroI MunI NheI
NotI NruI NsiI PacI PinAI
PmeI PshBI Psp1406I RsrII SalI
SbfI SfiI SgrAI SnaBI SpeI
Sse8387I SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 5630 GAATTT [GA]^AATT[CT]
ApoI 5630 GAATTT [GA]^AATT[CT]
AspI 3150 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1055 GGATCC G^GATCC
BbrPI 1503 CACGTG CAC^GTG
BcgI 380 CGAGGCGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1433 TGATCA T^GATCA
BsaWI 4957 ACCGGA [AT]^CCGG[AT]
BsmI 1661 GCATTC GAATGC(1/-1)
Bsp1407I 2780 TGTACA T^GTACA
BspHI 3850 TCATGA T^CATGA
BsrGI 2780 TGTACA T^GTACA
BstBI 1399 TTCGAA TT^CGAA
DrdI 5848 GACTACTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1433 TGATCA T^GATCA
FseI 751 GGCCGGCC GGCCGG^CC
HindIII 4926 AAGCTT A^AGCTT
NdeI 2905 CATATG CA^TATG
NspV 1399 TTCGAA TT^CGAA
PflFI 3150 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1503 CACGTG CAC^GTG
PmlI 1503 CACGTG CAC^GTG
PshAI 2061 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 5485 CGATCG CGAT^CG
RcaI 3850 TCATGA T^CATGA
SacI 5403 GAGCTC GAGCT^C
SexAI 5705 ACCAGGT A^CC[AT]GGT
SfuI 1399 TTCGAA TT^CGAA
SmaI 1411 CCCGGG CCC^GGG
SspBI 2780 TGTACA T^GTACA
TfiI 1972 GATTC G^A[AT]TC
Tth111I 3150 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1411 CCCGGG C^CCGGG
XmaI 1411 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 1415 GGTACC G^GTACC
2586 GGTACC
Asp718I 1415 GGTACC G^GTACC
2586 GGTACC
BlpI 5006 GCTCAGC GC^T[ACGT]AGC
5567 GCTCAGC
Bpu1102I 5006 GCTCAGC GC^T[ACGT]AGC
5567 GCTCAGC
BsaAI 104 CACGTA [CT]AC^GT[GA]
1503 CACGTG
BsaBI 182 GATCGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3956 GATGTCCATC
BsaI 1185 GAGACC GGTCTC(1/5)
2383 GGTCTC
BspDI 4872 ATCGAT AT^CGAT
5483 ATCGAT
BsrDI 5757 CATTGC GCAATG(2/0)
2863 GCAATG
BstAPI 682 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2563 GCATCACCTGC
BstEII 1062 GGTTACC G^GT[ACGT]ACC
2067 GGTCACC
BstPI 1062 GGTTACC G^GT[ACGT]ACC
2067 GGTCACC
CelII 5006 GCTCAGC GC^T[ACGT]AGC
5567 GCTCAGC
ClaI 4872 ATCGAT AT^CGAT
5483 ATCGAT
EcoO65I 1062 GGTTACC G^GT[ACGT]ACC
2067 GGTCACC
KpnI 1415 GGTACC GGTAC^C
2586 GGTACC
MamI 182 GATCGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3956 GATGTCCATC
MluI 34 ACGCGT A^CGCGT
358 ACGCGT
NaeI 701 GCCGGC GCC^GGC
752 GCCGGC
NgoMIV 701 GCCGGC G^CCGGC
752 GCCGGC
PaeR7I 1827 CTCGAG C^TCGAG
4301 CTCGAG
ScaI 1283 AGTACT AGT^ACT
3835 AGTACT
SphI 4 GCATGC GCATG^C
976 GCATGC
XhoI 1827 CTCGAG C^TCGAG
4301 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 146 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BglII BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssHII
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacII
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI