##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AccI AclI AhdI Alw44I ApaLI
AscI AseI AsnI AspEI AviII
BamHI BclI BlpI Bpu1102I BsiWI
BspLU11I Bst1107I BstBI BstEII BstPI
BstZ17I CelII Eam1105I EcoO65I FbaI
FseI FspI HpaI NaeI NdeI
NgoMIV NruI NspV PacI PaeR7I
PmeI PshBI Psp1406I SalI SapI
SbfI SfiI SfuI SgrAI SmaI
SpeI Sse8387I SwaI XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 3273 GGTACC G^GTACC
AccIII 5183 TCCGGA T^CCGGA
AflII 4264 CTTAAG C^TTAAG
AgeI 1027 ACCGGT A^CCGGT
AocI 2515 CCTGAGG CC^T[ACGT]AGG
Asp700I 2828 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3273 GGTACC G^GTACC
AvrII 169 CCTAGG C^CTAGG
BbrPI 3278 CACGTG CAC^GTG
BciVI 5267 GTATCC GTATCC(6/5)
BfrI 4264 CTTAAG C^TTAAG
BlnI 169 CCTAGG C^CTAGG
BsaBI 1706 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 5183 TCCGGA T^CCGGA
BspEI 5183 TCCGGA T^CCGGA
BspHI 2148 TCATGA T^CATGA
BssSI 3800 CACGAG CACGAG(-5/-1)
Bsu36I 2515 CCTGAGG CC^T[ACGT]AGG
CpoI 891 CGGTCCG CG^G[AT]CCG
Eco81I 2515 CCTGAGG CC^T[ACGT]AGG
EcoNI 1214 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4036 GAATTC G^AATTC
EcoRV 2360 GATATC GAT^ATC
HincII 2714 GTCAAC GT[CT]^[GA]AC
HindII 2714 GTCAAC GT[CT]^[GA]AC
HindIII 3163 AAGCTT A^AGCTT
KpnI 3273 GGTACC GGTAC^C
KspI 1579 CCGCGG CCGC^GG
MamI 1706 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3700 CAATTG C^AATTG
MluI 4853 ACGCGT A^CGCGT
MroI 5183 TCCGGA T^CCGGA
MunI 3700 CAATTG C^AATTG
NheI 964 GCTAGC G^CTAGC
NotI 865 GCGGCCGC GC^GGCCGC
PinAI 1027 ACCGGT A^CCGGT
PmaCI 3278 CACGTG CAC^GTG
PmlI 3278 CACGTG CAC^GTG
PshAI 3024 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2148 TCATGA T^CATGA
RsrII 891 CGGTCCG CG^G[AT]CCG
SacII 1579 CCGCGG CCGC^GG
SexAI 4895 ACCTGGT A^CC[AT]GGT
SnaBI 5011 TACGTA TAC^GTA
SspI 4516 AATATT AAT^ATT
XmnI 2828 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AatII 1514 GACGTC GACGT^C
3661 GACGTC
AflIII 4853 ACGCGT A^C[GA][CT]GT
5458 ACACGT
Aor51HI 247 AGCGCT AGC^GCT
5000 AGCGCT
AspI 2488 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3358 GACCGAGTC
BbeI 1595 GGCGCC GGCGC^C
2705 GGCGCC
BcgI 182 CGAGGCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5759 CGATTTTTTTGC
BglI 100 GCCTCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2789 GCCAAAGTGGC
BsaAI 3278 CACGTG [CT]AC^GT[GA]
5011 TACGTA
BsaWI 1027 ACCGGT [AT]^CCGG[AT]
5183 TCCGGA
BspDI 5791 ATCGAT AT^CGAT
5795 ATCGAT
BssHII 150 GCGCGC G^CGCGC
886 GCGCGC
BstAPI 67 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1389 GCAGTCCCTGC
BstXI 1605 CCAAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1930 CCAGGAGTTTGG
ClaI 5791 ATCGAT AT^CGAT
5795 ATCGAT
DrdI 3735 GACCCTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5566 GACATGAGCGTC
Eco47III 247 AGCGCT AGC^GCT
5000 AGCGCT
EcoT22I 2030 ATGCAT ATGCA^T
5968 ATGCAT
KasI 1595 GGCGCC G^GCGCC
2705 GGCGCC
MslI 555 CATTGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2960 CACCACAGTG
NarI 1595 GGCGCC GG^CGCC
2705 GGCGCC
NsiI 2030 ATGCAT ATGCA^T
5968 ATGCAT
PflFI 2488 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3358 GACCGAGTC
PflMI 221 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3754 CCAACTTATGG
PvuI 1 CGATCG CGAT^CG
5793 CGATCG
SacI 3049 GAGCTC GAGCT^C
4847 GAGCTC
ScaI 29 AGTACT AGT^ACT
2108 AGTACT
SfoI 1595 GGCGCC GGC^GCC
2705 GGCGCC
SphI 2973 GCATGC GCATG^C
4311 GCATGC
StuI 3181 AGGCCT AGG^CCT
5613 AGGCCT
Tth111I 2488 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3358 GACCGAGTC
Van91I 221 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3754 CCAACTTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII