################################## # NO CUT ENZYMES ( 52 enzymes) # ################################## AccI AclI AhdI Alw44I ApaLI AscI AseI AsnI AspEI AviII BamHI BclI BlpI Bpu1102I BsiWI BspLU11I Bst1107I BstBI BstEII BstPI BstZ17I CelII Eam1105I EcoO65I FbaI FseI FspI HpaI NaeI NdeI NgoMIV NruI NspV PacI PaeR7I PmeI PshBI Psp1406I SalI SapI SbfI SfiI SfuI SgrAI SmaI SpeI Sse8387I SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 3273 GGTACC G^GTACC AccIII 5183 TCCGGA T^CCGGA AflII 4264 CTTAAG C^TTAAG AgeI 1027 ACCGGT A^CCGGT AocI 2515 CCTGAGG CC^T[ACGT]AGG Asp700I 2828 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 3273 GGTACC G^GTACC AvrII 169 CCTAGG C^CTAGG BbrPI 3278 CACGTG CAC^GTG BciVI 5267 GTATCC GTATCC(6/5) BfrI 4264 CTTAAG C^TTAAG BlnI 169 CCTAGG C^CTAGG BsaBI 1706 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 5183 TCCGGA T^CCGGA BspEI 5183 TCCGGA T^CCGGA BspHI 2148 TCATGA T^CATGA BssSI 3800 CACGAG CACGAG(-5/-1) Bsu36I 2515 CCTGAGG CC^T[ACGT]AGG CpoI 891 CGGTCCG CG^G[AT]CCG Eco81I 2515 CCTGAGG CC^T[ACGT]AGG EcoNI 1214 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 4036 GAATTC G^AATTC EcoRV 2360 GATATC GAT^ATC HincII 2714 GTCAAC GT[CT]^[GA]AC HindII 2714 GTCAAC GT[CT]^[GA]AC HindIII 3163 AAGCTT A^AGCTT KpnI 3273 GGTACC GGTAC^C KspI 1579 CCGCGG CCGC^GG MamI 1706 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3700 CAATTG C^AATTG MluI 4853 ACGCGT A^CGCGT MroI 5183 TCCGGA T^CCGGA MunI 3700 CAATTG C^AATTG NheI 964 GCTAGC G^CTAGC NotI 865 GCGGCCGC GC^GGCCGC PinAI 1027 ACCGGT A^CCGGT PmaCI 3278 CACGTG CAC^GTG PmlI 3278 CACGTG CAC^GTG PshAI 3024 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 2148 TCATGA T^CATGA RsrII 891 CGGTCCG CG^G[AT]CCG SacII 1579 CCGCGG CCGC^GG SexAI 4895 ACCTGGT A^CC[AT]GGT SnaBI 5011 TACGTA TAC^GTA SspI 4516 AATATT AAT^ATT XmnI 2828 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AatII 1514 GACGTC GACGT^C 3661 GACGTC AflIII 4853 ACGCGT A^C[GA][CT]GT 5458 ACACGT Aor51HI 247 AGCGCT AGC^GCT 5000 AGCGCT AspI 2488 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3358 GACCGAGTC BbeI 1595 GGCGCC GGCGC^C 2705 GGCGCC BcgI 182 CGAGGCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5759 CGATTTTTTTGC BglI 100 GCCTCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2789 GCCAAAGTGGC BsaAI 3278 CACGTG [CT]AC^GT[GA] 5011 TACGTA BsaWI 1027 ACCGGT [AT]^CCGG[AT] 5183 TCCGGA BspDI 5791 ATCGAT AT^CGAT 5795 ATCGAT BssHII 150 GCGCGC G^CGCGC 886 GCGCGC BstAPI 67 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1389 GCAGTCCCTGC BstXI 1605 CCAAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1930 CCAGGAGTTTGG ClaI 5791 ATCGAT AT^CGAT 5795 ATCGAT DrdI 3735 GACCCTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5566 GACATGAGCGTC Eco47III 247 AGCGCT AGC^GCT 5000 AGCGCT EcoT22I 2030 ATGCAT ATGCA^T 5968 ATGCAT KasI 1595 GGCGCC G^GCGCC 2705 GGCGCC MslI 555 CATTGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2960 CACCACAGTG NarI 1595 GGCGCC GG^CGCC 2705 GGCGCC NsiI 2030 ATGCAT ATGCA^T 5968 ATGCAT PflFI 2488 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3358 GACCGAGTC PflMI 221 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3754 CCAACTTATGG PvuI 1 CGATCG CGAT^CG 5793 CGATCG SacI 3049 GAGCTC GAGCT^C 4847 GAGCTC ScaI 29 AGTACT AGT^ACT 2108 AGTACT SfoI 1595 GGCGCC GGC^GCC 2705 GGCGCC SphI 2973 GCATGC GCATG^C 4311 GCATGC StuI 3181 AGGCCT AGG^CCT 5613 AGGCCT Tth111I 2488 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3358 GACCGAGTC Van91I 221 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3754 CCAACTTATGG ######################################### # 3 OR MORE CUT ENZYMES ( 129 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII