##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
Alw44I ApaI ApaLI AscI Asp718I
AvrII BanI BbeI BciVI BlnI
BsaHI BsiWI BspDI BsrDI BssHII
BssSI Bst1107I BstZ17I ClaI CpoI
DraIII DrdI EagI EclXI Eco52I
FseI Hin1I KasI KpnI MfeI
MluI MunI NarI NheI NotI
NruI PacI PinAI PmeI PshAI
Psp1406I RsrII SbfI ScaI SfiI
SfoI SgrAI SnaBI Sse8387I SwaI
XbaI XcmI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AhdI 528 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 477 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2743 AGCGCT AGC^GCT
AspEI 528 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2460 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 784 TGCGCA TGC^GCA
BalI 3324 TGGCCA TGG^CCA
BcgI 603 GCAGCAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 3045 GCCTGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1858 AGATCT A^GATCT
BsiEI 657 CGATCG CG[GA][CT]^CG
BsmBI 87 GAGACG CGTCTC(1/5)
BspLU11I 4644 ACATGT A^CATGT
BspMI 4603 GCAGGT ACCTGC(4/8)
BsrBI 205 GAGCGG CCGCTC(-3/-3)
BsrFI 147 GCCGGC [GA]^CCGG[CT]
BstAPI 4812 GCAACAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2802 TTCGAA TT^CGAA
BstXI 4736 CCACTGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 477 CCTGAGG CC^T[ACGT]AGG
Cfr10I 147 GCCGGC [GA]^CCGG[CT]
Eam1105I 528 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2743 AGCGCT AGC^GCT
Eco81I 477 CCTGAGG CC^T[ACGT]AGG
EcoRI 2440 GAATTC G^AATTC
EcoRV 4715 GATATC GAT^ATC
EcoT22I 4959 ATGCAT ATGCA^T
FspI 784 TGCGCA TGC^GCA
HgaI 3076 GACGC GACGC(5/10)
KspI 645 CCGCGG CCGC^GG
MluNI 3324 TGGCCA TGG^CCA
MscI 3324 TGGCCA TGG^CCA
NaeI 147 GCCGGC GCC^GGC
NcoI 5691 CCATGG C^CATGG
NdeI 2386 CATATG CA^TATG
NgoMIV 147 GCCGGC G^CCGGC
NsiI 4959 ATGCAT ATGCA^T
NspV 2802 TTCGAA TT^CGAA
PaeR7I 1578 CTCGAG C^TCGAG
PflFI 2460 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 745 CCAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 657 CGATCG CGAT^CG
SacII 645 CCGCGG CCGC^GG
SapI 2196 GAAGAGC GCTCTTC(1/4)
SexAI 5606 ACCAGGT A^CC[AT]GGT
SfuI 2802 TTCGAA TT^CGAA
SmaI 112 CCCGGG CCC^GGG
SpeI 5445 ACTAGT A^CTAGT
SphI 3919 GCATGC GCATG^C
Tth111I 2460 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 745 CCAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1578 CTCGAG C^TCGAG
XmaCI 112 CCCGGG C^CCGGG
XmaI 112 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 389 CGCG CG^CG
646 CGCG
AccIII 249 TCCGGA T^CCGGA
3261 TCCGGA
AseI 3016 ATTAAT AT^TAAT
4246 ATTAAT
AsnI 3016 ATTAAT AT^TAAT
4246 ATTAAT
BamHI 134 GGATCC G^GATCC
3264 GGATCC
BbrPI 2781 CACGTG CAC^GTG
4679 CACGTG
BbvCI 2674 GCTGAGG CCTCAGC(-5/-2)
5661 GCTGAGG
BsaBI 4279 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4426 GATAAATATC
BsaI 2335 GAGACC GGTCTC(1/5)
3467 GGTCTC
BseAI 249 TCCGGA T^CCGGA
3261 TCCGGA
BspEI 249 TCCGGA T^CCGGA
3261 TCCGGA
BspHI 2400 TCATGA T^CATGA
3360 TCATGA
BstEII 2104 GGTTACC G^GT[ACGT]ACC
4752 GGTAACC
BstPI 2104 GGTTACC G^GT[ACGT]ACC
4752 GGTAACC
BstUI 389 CGCG CG^CG
646 CGCG
DsaI 645 CCGCGG C^C[GA][CT]GG
5691 CCATGG
EaeI 642 TGGCCG [CT]^GGCC[GA]
3324 TGGCCA
EcoNI 3839 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4975 CCTGGAACAGG
EcoO65I 2104 GGTTACC G^GT[ACGT]ACC
4752 GGTAACC
EcoT14I 2692 CCAAGG C^C[AT][AT]GG
5691 CCATGG
HaeII 5 GGCGCT [GA]GCGC^[CT]
2743 AGCGCT
HpaI 2076 GTTAAC GTT^AAC
3225 GTTAAC
MamI 4279 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4426 GATAAATATC
MroI 249 TCCGGA T^CCGGA
3261 TCCGGA
MvnI 389 CGCG CG^CG
646 CGCG
PmaCI 2781 CACGTG CAC^GTG
4679 CACGTG
PmlI 2781 CACGTG CAC^GTG
4679 CACGTG
PshBI 3016 ATTAAT AT^TAAT
4246 ATTAAT
PvuII 968 CAGCTG CAG^CTG
2672 CAGCTG
RcaI 2400 TCATGA T^CATGA
3360 TCATGA
SalI 1940 GTCGAC G^TCGAC
3520 GTCGAC
StyI 2692 CCAAGG C^C[AT][AT]GG
5691 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BanII
BbsI BbvI BclI BcnI BfaI
BfrI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRII FauI FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StuI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI