################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## AatII Acc65I AclI AcyI AgeI Alw44I ApaI ApaLI AscI Asp718I AvrII BanI BbeI BciVI BlnI BsaHI BsiWI BspDI BsrDI BssHII BssSI Bst1107I BstZ17I ClaI CpoI DraIII DrdI EagI EclXI Eco52I FseI Hin1I KasI KpnI MfeI MluI MunI NarI NheI NotI NruI PacI PinAI PmeI PshAI Psp1406I RsrII SbfI ScaI SfiI SfoI SgrAI SnaBI Sse8387I SwaI XbaI XcmI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AhdI 528 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 477 CCTGAGG CC^T[ACGT]AGG Aor51HI 2743 AGCGCT AGC^GCT AspEI 528 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2460 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 784 TGCGCA TGC^GCA BalI 3324 TGGCCA TGG^CCA BcgI 603 GCAGCAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 3045 GCCTGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1858 AGATCT A^GATCT BsiEI 657 CGATCG CG[GA][CT]^CG BsmBI 87 GAGACG CGTCTC(1/5) BspLU11I 4644 ACATGT A^CATGT BspMI 4603 GCAGGT ACCTGC(4/8) BsrBI 205 GAGCGG CCGCTC(-3/-3) BsrFI 147 GCCGGC [GA]^CCGG[CT] BstAPI 4812 GCAACAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 2802 TTCGAA TT^CGAA BstXI 4736 CCACTGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 477 CCTGAGG CC^T[ACGT]AGG Cfr10I 147 GCCGGC [GA]^CCGG[CT] Eam1105I 528 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2743 AGCGCT AGC^GCT Eco81I 477 CCTGAGG CC^T[ACGT]AGG EcoRI 2440 GAATTC G^AATTC EcoRV 4715 GATATC GAT^ATC EcoT22I 4959 ATGCAT ATGCA^T FspI 784 TGCGCA TGC^GCA HgaI 3076 GACGC GACGC(5/10) KspI 645 CCGCGG CCGC^GG MluNI 3324 TGGCCA TGG^CCA MscI 3324 TGGCCA TGG^CCA NaeI 147 GCCGGC GCC^GGC NcoI 5691 CCATGG C^CATGG NdeI 2386 CATATG CA^TATG NgoMIV 147 GCCGGC G^CCGGC NsiI 4959 ATGCAT ATGCA^T NspV 2802 TTCGAA TT^CGAA PaeR7I 1578 CTCGAG C^TCGAG PflFI 2460 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 745 CCAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuI 657 CGATCG CGAT^CG SacII 645 CCGCGG CCGC^GG SapI 2196 GAAGAGC GCTCTTC(1/4) SexAI 5606 ACCAGGT A^CC[AT]GGT SfuI 2802 TTCGAA TT^CGAA SmaI 112 CCCGGG CCC^GGG SpeI 5445 ACTAGT A^CTAGT SphI 3919 GCATGC GCATG^C Tth111I 2460 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 745 CCAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1578 CTCGAG C^TCGAG XmaCI 112 CCCGGG C^CCGGG XmaI 112 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 389 CGCG CG^CG 646 CGCG AccIII 249 TCCGGA T^CCGGA 3261 TCCGGA AseI 3016 ATTAAT AT^TAAT 4246 ATTAAT AsnI 3016 ATTAAT AT^TAAT 4246 ATTAAT BamHI 134 GGATCC G^GATCC 3264 GGATCC BbrPI 2781 CACGTG CAC^GTG 4679 CACGTG BbvCI 2674 GCTGAGG CCTCAGC(-5/-2) 5661 GCTGAGG BsaBI 4279 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4426 GATAAATATC BsaI 2335 GAGACC GGTCTC(1/5) 3467 GGTCTC BseAI 249 TCCGGA T^CCGGA 3261 TCCGGA BspEI 249 TCCGGA T^CCGGA 3261 TCCGGA BspHI 2400 TCATGA T^CATGA 3360 TCATGA BstEII 2104 GGTTACC G^GT[ACGT]ACC 4752 GGTAACC BstPI 2104 GGTTACC G^GT[ACGT]ACC 4752 GGTAACC BstUI 389 CGCG CG^CG 646 CGCG DsaI 645 CCGCGG C^C[GA][CT]GG 5691 CCATGG EaeI 642 TGGCCG [CT]^GGCC[GA] 3324 TGGCCA EcoNI 3839 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4975 CCTGGAACAGG EcoO65I 2104 GGTTACC G^GT[ACGT]ACC 4752 GGTAACC EcoT14I 2692 CCAAGG C^C[AT][AT]GG 5691 CCATGG HaeII 5 GGCGCT [GA]GCGC^[CT] 2743 AGCGCT HpaI 2076 GTTAAC GTT^AAC 3225 GTTAAC MamI 4279 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4426 GATAAATATC MroI 249 TCCGGA T^CCGGA 3261 TCCGGA MvnI 389 CGCG CG^CG 646 CGCG PmaCI 2781 CACGTG CAC^GTG 4679 CACGTG PmlI 2781 CACGTG CAC^GTG 4679 CACGTG PshBI 3016 ATTAAT AT^TAAT 4246 ATTAAT PvuII 968 CAGCTG CAG^CTG 2672 CAGCTG RcaI 2400 TCATGA T^CATGA 3360 TCATGA SalI 1940 GTCGAC G^TCGAC 3520 GTCGAC StyI 2692 CCAAGG C^C[AT][AT]GG 5691 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AccI AciI AcsI AfaI AflII AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BanII BbsI BbvI BclI BcnI BfaI BfrI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII FauI FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StuI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI