##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AatII AclI Aor51HI AscI AseI
AsnI BclI BsaBI BsiWI Bsp1407I
BspDI BsrGI BssHII BstBI ClaI
Eco47III EcoRI EcoRV EcoT22I FbaI
FseI MamI MfeI MluI MunI
NotI NruI NsiI NspV PacI
PaeR7I PmeI PshBI Psp1406I PvuI
SacI SalI ScaI SfiI SfuI
SgrAI SnaBI SspBI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 4648 GGTACC G^GTACC
AccIII 45 TCCGGA T^CCGGA
AgeI 408 ACCGGT A^CCGGT
AhdI 1534 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 935 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4648 GGTACC G^GTACC
AspEI 1534 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1495 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 1344 CGAGTGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 5677 AGATCT A^GATCT
BseAI 45 TCCGGA T^CCGGA
BsmBI 392 CGTCTC CGTCTC(1/5)
BspEI 45 TCCGGA T^CCGGA
BspHI 1860 TCATGA T^CATGA
BssSI 4019 CACGAG CACGAG(-5/-1)
Bst1107I 1990 GTATAC GTA^TAC
BstAPI 2684 GCATCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1990 GTATAC GTA^TAC
DraI 783 TTTAAA TTT^AAA
DraIII 1723 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1534 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HindIII 1602 AAGCTT A^AGCTT
HpaI 2669 GTTAAC GTT^AAC
KpnI 4648 GGTACC GGTAC^C
MroI 45 TCCGGA T^CCGGA
NcoI 1954 CCATGG C^CATGG
NdeI 5868 CATATG CA^TATG
NheI 4990 GCTAGC G^CTAGC
PflFI 1495 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 408 ACCGGT A^CCGGT
PshAI 818 GACTGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1860 TCATGA T^CATGA
SapI 2408 GCTCTTC GCTCTTC(1/4)
SexAI 5798 ACCAGGT A^CC[AT]GGT
SpeI 1105 ACTAGT A^CTAGT
SphI 1356 GCATGC GCATG^C
SwaI 782 ATTTAAAT ATTT^AAAT
Tth111I 1495 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 935 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
ApaI 419 GGGCCC GGGCC^C
3884 GGGCCC
BbeI 63 GGCGCC GGCGC^C
121 GGCGCC
BbrPI 2238 CACGTG CAC^GTG
5725 CACGTG
BbvCI 4582 CCTCAGC CCTCAGC(-5/-2)
4985 CCTCAGC
BsaAI 2238 CACGTG [CT]AC^GT[GA]
5725 CACGTG
BsmI 3561 GCATTC GAATGC(1/-1)
3683 GCATTC
CpoI 178 CGGACCG CG^G[AT]CCG
410 CGGTCCG
EagI 107 CGGCCG C^GGCCG
388 CGGCCG
EclXI 107 CGGCCG C^GGCCG
388 CGGCCG
Eco52I 107 CGGCCG C^GGCCG
388 CGGCCG
HincII 1531 GTTGAC GT[CT]^[GA]AC
2669 GTTAAC
HindII 1531 GTTGAC GT[CT]^[GA]AC
2669 GTTAAC
KasI 63 GGCGCC G^GCGCC
121 GGCGCC
KspI 285 CCGCGG CCGC^GG
424 CCGCGG
NarI 63 GGCGCC GG^CGCC
121 GGCGCC
PmaCI 2238 CACGTG CAC^GTG
5725 CACGTG
PmlI 2238 CACGTG CAC^GTG
5725 CACGTG
RsrII 178 CGGACCG CG^G[AT]CCG
410 CGGTCCG
SacII 285 CCGCGG CCGC^GG
424 CCGCGG
SbfI 4100 CCTGCAGG CCTGCA^GG
4426 CCTGCAGG
SfoI 63 GGCGCC GGC^GCC
121 GGCGCC
Sse8387I 4100 CCTGCAGG CCTGCA^GG
4426 CCTGCAGG
SspI 3810 AATATT AAT^ATT
5685 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 151 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AflIII AluI Alw44I
AlwI AlwNI AocI ApaLI ApoI
AspHI AvaI AvaII AviII AvrII
BalI BamHI BanI BanII BbsI
BbvI BciVI BcnI BfaI BfrI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI