##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AclI AflII AgeI AscI
AseI AsnI AviII AvrII BbrPI
BfrI BlnI BsaBI BsiEI BsiWI
BssHII BssSI BstBI CpoI DraIII
EagI EclXI Eco52I EcoRV FseI
FspI KspI MamI MfeI MluI
MunI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 1378 GGTACC G^GTACC
AccI 2384 GTATAC GT^[AC][GT]AC
AccII 3 CGCG CG^CG
AccIII 1128 TCCGGA T^CCGGA
AcyI 109 GGCGCC G[GA]^CG[CT]C
AflIII 3372 ACATGT A^C[GA][CT]GT
AhdI 3294 GACTCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4280 AGCGCT AGC^GCT
Asp718I 1378 GGTACC G^GTACC
AspEI 3294 GACTCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5267 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 4907 CTCGGG C^[CT]CG[GA]G
BbeI 109 GGCGCC GGCGC^C
BbvCI 5348 CCTCAGC CCTCAGC(-5/-2)
BcgI 3109 CGAATCCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4465 TGATCA T^GATCA
BglI 4145 GCCTGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 155 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 155 GCTCAGC GC^T[ACGT]AGC
BsaAI 471 CACGTA [CT]AC^GT[GA]
BsaHI 109 GGCGCC G[GA]^CG[CT]C
BsaWI 1128 TCCGGA [AT]^CCGG[AT]
BseAI 1128 TCCGGA T^CCGGA
BsmBI 3078 GAGACG CGTCTC(1/5)
BsoBI 4907 CTCGGG C^[CT]CG[GA]G
Bsp1407I 422 TGTACA T^GTACA
BspDI 4300 ATCGAT AT^CGAT
BspEI 1128 TCCGGA T^CCGGA
BspHI 800 TCATGA T^CATGA
BspLU11I 3372 ACATGT A^CATGT
BsrBI 1441 GAGCGG CCGCTC(-3/-3)
BsrGI 422 TGTACA T^GTACA
Bst1107I 2384 GTATAC GTA^TAC
BstAPI 3681 GCAGTAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 3 CGCG CG^CG
BstZ17I 2384 GTATAC GTA^TAC
CelII 155 GCTCAGC GC^T[ACGT]AGC
ClaI 4300 ATCGAT AT^CGAT
DrdI 2255 GACTTGAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3294 GACTCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4280 AGCGCT AGC^GCT
FbaI 4465 TGATCA T^GATCA
Hin1I 109 GGCGCC G[GA]^CG[CT]C
KasI 109 GGCGCC G^GCGCC
KpnI 1378 GGTACC GGTAC^C
MroI 1128 TCCGGA T^CCGGA
MvnI 3 CGCG CG^CG
NarI 109 GGCGCC GG^CGCC
NheI 3404 GCTAGC G^CTAGC
PflFI 5267 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4905 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 800 TCATGA T^CATGA
SapI 3926 GCTCTTC GCTCTTC(1/4)
ScaI 1495 AGTACT AGT^ACT
SfoI 109 GGCGCC GGC^GCC
SspBI 422 TGTACA T^GTACA
SspI 1829 AATATT AAT^ATT
StuI 4735 AGGCCT AGG^CCT
SwaI 1824 ATTTAAAT ATTT^AAAT
Tth111I 5267 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2466 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Alw44I 954 GTGCAC G^TGCAC
2172 GTGCAC
ApaI 76 GGGCCC GGGCC^C
829 GGGCCC
ApaLI 954 GTGCAC G^TGCAC
2172 GTGCAC
Asp700I 1326 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2704 GAAACTGTTC
BamHI 1800 GGATCC G^GATCC
4797 GGATCC
BglII 2881 AGATCT A^GATCT
3127 AGATCT
BsrDI 945 CATTGC GCAATG(2/0)
4352 CATTGC
BsrFI 136 GCCGGC [GA]^CCGG[CT]
4420 GCCGGC
BstEII 1481 GGTTACC G^GT[ACGT]ACC
4504 GGTTACC
BstPI 1481 GGTTACC G^GT[ACGT]ACC
4504 GGTTACC
BstXI 3214 CCACTGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5728 CCATGTCTGTGG
CfoI 110 GCGC GCG^C
4281 GCGC
Cfr10I 136 GCCGGC [GA]^CCGG[CT]
4420 GCCGGC
EcoO65I 1481 GGTTACC G^GT[ACGT]ACC
4504 GGTTACC
EcoT22I 1718 ATGCAT ATGCA^T
4349 ATGCAT
HaeII 109 GGCGCC [GA]GCGC^[CT]
4280 AGCGCT
HgaI 870 GACGC GACGC(5/10)
3080 GACGC
HhaI 110 GCGC GCG^C
4281 GCGC
HinP1I 110 GCGC G^CGC
4281 GCGC
HindIII 2075 AAGCTT A^AGCTT
2391 AAGCTT
HpaI 409 GTTAAC GTT^AAC
5617 GTTAAC
NaeI 136 GCCGGC GCC^GGC
4420 GCCGGC
NdeI 4612 CATATG CA^TATG
5330 CATATG
NgoMIV 136 GCCGGC G^CCGGC
4420 GCCGGC
NsiI 1718 ATGCAT ATGCA^T
4349 ATGCAT
PflMI 2557 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4306 CCAGAAGCTGG
SacI 4227 GAGCTC GAGCT^C
4965 GAGCTC
Van91I 2557 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4306 CCAGAAGCTGG
XcmI 4802 CCAGAGTTAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4830 CCACAGCTCTTGTGG
XmnI 1326 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2704 GAAACTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BalI BanI BanII BbsI BbvI
BciVI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII