################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII AccII AccIII AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbeI BcgI BfrI BlnI BlpI Bpu1102I BsaWI BseAI BsiEI BsiWI BspDI BspEI BssHII BstUI CelII ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoRI FseI FspI HindIII HpaI KasI KspI MluI MroI MvnI NaeI NarI NgoMIV NotI NruI PacI PflFI PinAI PmeI PshAI PshBI PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SgrAI SpeI Sse8387I SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AclI 3914 AACGTT AA^CGTT AcyI 4906 GACGCC G[GA]^CG[CT]C BbrPI 3931 CACGTG CAC^GTG BciVI 4511 GTATCC GTATCC(6/5) BsaBI 1521 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 4906 GACGCC G[GA]^CG[CT]C BsmBI 2920 GAGACG CGTCTC(1/5) BsrFI 2780 GCCGGT [GA]^CCGG[CT] BssSI 5096 CACGAG CACGAG(-5/-1) Bst1107I 1558 GTATAC GTA^TAC BstBI 3338 TTCGAA TT^CGAA BstZ17I 1558 GTATAC GTA^TAC Cfr10I 2780 GCCGGT [GA]^CCGG[CT] DraI 5449 TTTAAA TTT^AAA DrdI 4825 GACTATGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HaeII 4714 GGCGCT [GA]GCGC^[CT] HgaI 4906 GACGC GACGC(5/10) Hin1I 4906 GACGCC G[GA]^CG[CT]C MamI 1521 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 5003 CAATTG C^AATTG MunI 5003 CAATTG C^AATTG NdeI 1907 CATATG CA^TATG NheI 2623 GCTAGC G^CTAGC NspV 3338 TTCGAA TT^CGAA PaeR7I 1823 CTCGAG C^TCGAG PmaCI 3931 CACGTG CAC^GTG PmlI 3931 CACGTG CAC^GTG Psp1406I 3914 AACGTT AA^CGTT SacI 5033 GAGCTC GAGCT^C SexAI 3971 ACCTGGT A^CC[AT]GGT SfuI 3338 TTCGAA TT^CGAA SnaBI 4804 TACGTA TAC^GTA SspI 4378 AATATT AAT^ATT XhoI 1823 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 4270 AAATTT [GA]^AATT[CT] 6163 AAATTT AflIII 1812 ACATGT A^C[GA][CT]GT 3978 ACATGT ApoI 4270 AAATTT [GA]^AATT[CT] 6163 AAATTT BamHI 1378 GGATCC G^GATCC 2694 GGATCC BbvCI 4398 CCTCAGC CCTCAGC(-5/-2) 568 GCTGAGG BclI 3732 TGATCA T^GATCA 4028 TGATCA BglII 1526 AGATCT A^GATCT 4760 AGATCT BsaAI 3931 CACGTG [CT]AC^GT[GA] 4804 TACGTA BspLU11I 1812 ACATGT A^CATGT 3978 ACATGT BsrBI 5995 CCGCTC CCGCTC(-3/-3) 1450 GAGCGG BstAPI 4966 GCATCTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5002 GCAATTGATGC CfoI 3087 GCGC GCG^C 4715 GCGC EcoNI 5613 CCTAAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5769 CCTGCTCAAGG EcoRV 412 GATATC GAT^ATC 3823 GATATC FbaI 3732 TGATCA T^GATCA 4028 TGATCA HhaI 3087 GCGC GCG^C 4715 GCGC HinP1I 3087 GCGC G^CGC 4715 GCGC SapI 5637 GAAGAGC GCTCTTC(1/4) 5997 GCTCTTC SmaI 1784 CCCGGG CCC^GGG 2006 CCCGGG SphI 39 GCATGC GCATG^C 5553 GCATGC XmaCI 1784 CCCGGG C^CCGGG 2006 CCCGGG XmaI 1784 CCCGGG C^CCGGG 2006 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 129 enzymes) # ######################################### Acc65I AccI AciI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI Asp718I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspHI BspMI BsrDI BsrGI BsrI BssKI BstEII BstF5I BstNI BstPI BstXI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoO109I EcoO65I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII