##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccII AccIII AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BbeI BcgI BfrI BlnI BlpI
Bpu1102I BsaWI BseAI BsiEI BsiWI
BspDI BspEI BssHII BstUI CelII
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI FseI FspI
HindIII HpaI KasI KspI MluI
MroI MvnI NaeI NarI NgoMIV
NotI NruI PacI PflFI PinAI
PmeI PshAI PshBI PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SpeI Sse8387I SwaI
Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AclI 3914 AACGTT AA^CGTT
AcyI 4906 GACGCC G[GA]^CG[CT]C
BbrPI 3931 CACGTG CAC^GTG
BciVI 4511 GTATCC GTATCC(6/5)
BsaBI 1521 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4906 GACGCC G[GA]^CG[CT]C
BsmBI 2920 GAGACG CGTCTC(1/5)
BsrFI 2780 GCCGGT [GA]^CCGG[CT]
BssSI 5096 CACGAG CACGAG(-5/-1)
Bst1107I 1558 GTATAC GTA^TAC
BstBI 3338 TTCGAA TT^CGAA
BstZ17I 1558 GTATAC GTA^TAC
Cfr10I 2780 GCCGGT [GA]^CCGG[CT]
DraI 5449 TTTAAA TTT^AAA
DrdI 4825 GACTATGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 4714 GGCGCT [GA]GCGC^[CT]
HgaI 4906 GACGC GACGC(5/10)
Hin1I 4906 GACGCC G[GA]^CG[CT]C
MamI 1521 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5003 CAATTG C^AATTG
MunI 5003 CAATTG C^AATTG
NdeI 1907 CATATG CA^TATG
NheI 2623 GCTAGC G^CTAGC
NspV 3338 TTCGAA TT^CGAA
PaeR7I 1823 CTCGAG C^TCGAG
PmaCI 3931 CACGTG CAC^GTG
PmlI 3931 CACGTG CAC^GTG
Psp1406I 3914 AACGTT AA^CGTT
SacI 5033 GAGCTC GAGCT^C
SexAI 3971 ACCTGGT A^CC[AT]GGT
SfuI 3338 TTCGAA TT^CGAA
SnaBI 4804 TACGTA TAC^GTA
SspI 4378 AATATT AAT^ATT
XhoI 1823 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 4270 AAATTT [GA]^AATT[CT]
6163 AAATTT
AflIII 1812 ACATGT A^C[GA][CT]GT
3978 ACATGT
ApoI 4270 AAATTT [GA]^AATT[CT]
6163 AAATTT
BamHI 1378 GGATCC G^GATCC
2694 GGATCC
BbvCI 4398 CCTCAGC CCTCAGC(-5/-2)
568 GCTGAGG
BclI 3732 TGATCA T^GATCA
4028 TGATCA
BglII 1526 AGATCT A^GATCT
4760 AGATCT
BsaAI 3931 CACGTG [CT]AC^GT[GA]
4804 TACGTA
BspLU11I 1812 ACATGT A^CATGT
3978 ACATGT
BsrBI 5995 CCGCTC CCGCTC(-3/-3)
1450 GAGCGG
BstAPI 4966 GCATCTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5002 GCAATTGATGC
CfoI 3087 GCGC GCG^C
4715 GCGC
EcoNI 5613 CCTAAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5769 CCTGCTCAAGG
EcoRV 412 GATATC GAT^ATC
3823 GATATC
FbaI 3732 TGATCA T^GATCA
4028 TGATCA
HhaI 3087 GCGC GCG^C
4715 GCGC
HinP1I 3087 GCGC G^CGC
4715 GCGC
SapI 5637 GAAGAGC GCTCTTC(1/4)
5997 GCTCTTC
SmaI 1784 CCCGGG CCC^GGG
2006 CCCGGG
SphI 39 GCATGC GCATG^C
5553 GCATGC
XmaCI 1784 CCCGGG C^CCGGG
2006 CCCGGG
XmaI 1784 CCCGGG C^CCGGG
2006 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
Acc65I AccI AciI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI Asp718I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspHI
BspMI BsrDI BsrGI BsrI BssKI
BstEII BstF5I BstNI BstPI BstXI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoO109I EcoO65I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII