##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AclI Alw44I Aor51HI ApaLI
AscI AseI AsnI AviII AvrII
BamHI BbrPI BciVI BclI BglII
BlnI BsaBI BsiWI BsmI BspDI
BspHI BspLU11I BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DraIII Eco47III EcoT22I FbaI FspI
HindIII HpaI MamI MfeI MluI
MunI NotI NruI NsiI NspV
PacI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SexAI SfiI SfuI SgrAI
SnaBI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccI 3157 GTAGAC GT^[AC][GT]AC
AccIII 1541 TCCGGA T^CCGGA
AgeI 4340 ACCGGT A^CCGGT
AhdI 4897 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1345 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4897 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 289 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BsaAI 4357 TACGTG [CT]AC^GT[GA]
BseAI 1541 TCCGGA T^CCGGA
BspEI 1541 TCCGGA T^CCGGA
DrdI 5597 GACAGAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4897 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 5305 GAATTC G^AATTC
EcoRV 2755 GATATC GAT^ATC
FseI 314 GGCCGGCC GGCCGG^CC
HincII 4378 GTCAAC GT[CT]^[GA]AC
HindII 4378 GTCAAC GT[CT]^[GA]AC
MroI 1541 TCCGGA T^CCGGA
MslI 671 CACCCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 1490 CATATG CA^TATG
NspI 5459 GCATGC [GA]CATG^[CT]
PaeR7I 3532 CTCGAG C^TCGAG
PflFI 289 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4340 ACCGGT A^CCGGT
PshAI 5428 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1030 AGTACT AGT^ACT
SpeI 1546 ACTAGT A^CTAGT
SphI 5459 GCATGC GCATG^C
TfiI 2943 GATTC G^A[AT]TC
Tth111I 289 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3532 CTCGAG C^TCGAG
XmnI 1345 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 4258 AAATTC [GA]^AATT[CT]
5305 GAATTC
AflII 1197 CTTAAG C^TTAAG
3085 CTTAAG
AflIII 3656 ACACGT A^C[GA][CT]GT
5168 ACACGT
AocI 1394 CCTCAGG CC^T[ACGT]AGG
3649 CCTAAGG
ApoI 4258 AAATTC [GA]^AATT[CT]
5305 GAATTC
BcgI 4593 CGAGCCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5412 CGAGGGTGGTGC
BfrI 1197 CTTAAG C^TTAAG
3085 CTTAAG
BsiEI 313 CGGCCG CG[GA][CT]^CG
1574 CGGCCG
Bsp1407I 4982 TGTACA T^GTACA
5722 TGTACA
BsrDI 2087 CATTGC GCAATG(2/0)
2231 CATTGC
BsrGI 4982 TGTACA T^GTACA
5722 TGTACA
BstAPI 2061 GCAAGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5454 GCAGTGCATGC
BstEII 4683 GGTAACC G^GT[ACGT]ACC
5543 GGTGACC
BstPI 4683 GGTAACC G^GT[ACGT]ACC
5543 GGTGACC
Bsu36I 1394 CCTCAGG CC^T[ACGT]AGG
3649 CCTAAGG
EagI 313 CGGCCG C^GGCCG
1574 CGGCCG
EclXI 313 CGGCCG C^GGCCG
1574 CGGCCG
Eco52I 313 CGGCCG C^GGCCG
1574 CGGCCG
Eco81I 1394 CCTCAGG CC^T[ACGT]AGG
3649 CCTAAGG
EcoO65I 4683 GGTAACC G^GT[ACGT]ACC
5543 GGTGACC
MseI 1198 TTAA T^TAA
3086 TTAA
SapI 1713 GAAGAGC GCTCTTC(1/4)
3514 GAAGAGC
SmaI 1913 CCCGGG CCC^GGG
3188 CCCGGG
SspBI 4982 TGTACA T^GTACA
5722 TGTACA
StuI 2439 AGGCCT AGG^CCT
3372 AGGCCT
Tru9I 1198 TTAA T^TAA
3086 TTAA
XmaCI 1913 CCCGGG C^CCGGG
3188 CCCGGG
XmaI 1913 CCCGGG C^CCGGG
3188 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI Asp718I
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI KpnI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NheI NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacII
Sau3AI Sau96I SbfI ScrFI SfaNI
SfcI SfoI SmlI Sse8387I StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII