##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AflII AflIII Alw44I AocI ApaI
ApaLI AscI AspI AviII BfrI
BsgI BsiEI BsiWI BspDI BspLU11I
BsrBI BssHII Bst1107I BstZ17I Bsu36I
ClaI CpoI DraI DraIII EagI
EclXI Eco52I Eco81I EcoRI EcoT22I
FspI KspI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
NspI PacI PflFI PmeI PvuI
RsrII SacII SbfI ScaI SexAI
SgrAI SmaI SnaBI SphI Sse8387I
SspI SwaI Tth111I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 1030 GACGTC GACGT^C
Acc65I 385 GGTACC G^GTACC
AclI 2065 AACGTT AA^CGTT
AcsI 4653 AAATTT [GA]^AATT[CT]
AgeI 1255 ACCGGT A^CCGGT
Aor51HI 3988 AGCGCT AGC^GCT
ApoI 4653 AAATTT [GA]^AATT[CT]
AseI 1649 ATTAAT AT^TAAT
AsnI 1649 ATTAAT AT^TAAT
Asp718I 385 GGTACC G^GTACC
BbeI 4060 GGCGCC GGCGC^C
BbrPI 3791 CACGTG CAC^GTG
BbvCI 3624 CCTCAGC CCTCAGC(-5/-2)
BcgI 3659 GCAGCAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2455 GTATCC GTATCC(6/5)
BlpI 3079 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3079 GCTCAGC GC^T[ACGT]AGC
BsaAI 3791 CACGTG [CT]AC^GT[GA]
BsmI 2667 GAATGC GAATGC(1/-1)
Bsp1407I 1221 TGTACA T^GTACA
BspMI 366 ACCTGC ACCTGC(4/8)
BsrGI 1221 TGTACA T^GTACA
BssSI 4857 CTCGTG CACGAG(-5/-1)
BstBI 5015 TTCGAA TT^CGAA
BstEII 17 GGTGACC G^GT[ACGT]ACC
BstPI 17 GGTGACC G^GT[ACGT]ACC
BstXI 1566 CCAGCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 3079 GCTCAGC GC^T[ACGT]AGC
Eco47III 3988 AGCGCT AGC^GCT
EcoO65I 17 GGTGACC G^GT[ACGT]ACC
EcoRV 2818 GATATC GAT^ATC
FseI 3869 GGCCGGCC GGCCGG^CC
KasI 4060 GGCGCC G^GCGCC
KpnI 385 GGTACC GGTAC^C
NaeI 3870 GCCGGC GCC^GGC
NarI 4060 GGCGCC GG^CGCC
NcoI 1020 CCATGG C^CATGG
NgoMIV 3870 GCCGGC G^CCGGC
NspV 5015 TTCGAA TT^CGAA
PaeR7I 903 CTCGAG C^TCGAG
PinAI 1255 ACCGGT A^CCGGT
PmaCI 3791 CACGTG CAC^GTG
PmlI 3791 CACGTG CAC^GTG
PshAI 98 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1649 ATTAAT AT^TAAT
Psp1406I 2065 AACGTT AA^CGTT
PvuII 4661 CAGCTG CAG^CTG
SacI 4372 GAGCTC GAGCT^C
SalI 5123 GTCGAC G^TCGAC
SfiI 1576 GGCCCTAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 4060 GGCGCC GGC^GCC
SfuI 5015 TTCGAA TT^CGAA
SpeI 1604 ACTAGT A^CTAGT
SspBI 1221 TGTACA T^GTACA
XcmI 3774 CCAAGAAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 903 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 3205 GTCTAC GT^[AC][GT]AC
5123 GTCGAC
AccII 2354 CGCG CG^CG
4129 CGCG
AhdI 2546 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3197 GACACCCTGTC
AspEI 2546 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3197 GACACCCTGTC
BsaBI 721 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4965 GATACACATC
BsaI 3912 GAGACC GGTCTC(1/5)
3026 GGTCTC
BspHI 1026 TCATGA T^CATGA
2766 TCATGA
BstAPI 638 GCAGACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1288 GCAACCTCTGC
BstUI 2354 CGCG CG^CG
4129 CGCG
DrdI 899 GACCCTCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5126 GACCCAAGTGTC
Eam1105I 2546 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3197 GACACCCTGTC
EcoNI 4150 CCTTCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4216 CCTTCCCTAGG
HaeII 3988 AGCGCT [GA]GCGC^[CT]
4060 GGCGCC
HpaI 4782 GTTAAC GTT^AAC
4940 GTTAAC
MamI 721 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4965 GATACACATC
MvnI 2354 CGCG CG^CG
4129 CGCG
RcaI 1026 TCATGA T^CATGA
2766 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccIII AciI AcyI AfaI AluI
AlwI AlwNI Asp700I AspHI AvaI
AvaII AvrII BalI BamHI BanI
BanII BbsI BbvI BclI BcnI
BfaI BglI BglII BlnI BmyI
BpmI BpuAI BsaHI BsaJI BsaWI
BseAI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspEI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI