################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AflII AflIII Alw44I AocI ApaI ApaLI AscI AspI AviII BfrI BsgI BsiEI BsiWI BspDI BspLU11I BsrBI BssHII Bst1107I BstZ17I Bsu36I ClaI CpoI DraI DraIII EagI EclXI Eco52I Eco81I EcoRI EcoT22I FspI KspI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspI PacI PflFI PmeI PvuI RsrII SacII SbfI ScaI SexAI SgrAI SmaI SnaBI SphI Sse8387I SspI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AatII 1030 GACGTC GACGT^C Acc65I 385 GGTACC G^GTACC AclI 2065 AACGTT AA^CGTT AcsI 4653 AAATTT [GA]^AATT[CT] AgeI 1255 ACCGGT A^CCGGT Aor51HI 3988 AGCGCT AGC^GCT ApoI 4653 AAATTT [GA]^AATT[CT] AseI 1649 ATTAAT AT^TAAT AsnI 1649 ATTAAT AT^TAAT Asp718I 385 GGTACC G^GTACC BbeI 4060 GGCGCC GGCGC^C BbrPI 3791 CACGTG CAC^GTG BbvCI 3624 CCTCAGC CCTCAGC(-5/-2) BcgI 3659 GCAGCAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2455 GTATCC GTATCC(6/5) BlpI 3079 GCTCAGC GC^T[ACGT]AGC Bpu1102I 3079 GCTCAGC GC^T[ACGT]AGC BsaAI 3791 CACGTG [CT]AC^GT[GA] BsmI 2667 GAATGC GAATGC(1/-1) Bsp1407I 1221 TGTACA T^GTACA BspMI 366 ACCTGC ACCTGC(4/8) BsrGI 1221 TGTACA T^GTACA BssSI 4857 CTCGTG CACGAG(-5/-1) BstBI 5015 TTCGAA TT^CGAA BstEII 17 GGTGACC G^GT[ACGT]ACC BstPI 17 GGTGACC G^GT[ACGT]ACC BstXI 1566 CCAGCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 3079 GCTCAGC GC^T[ACGT]AGC Eco47III 3988 AGCGCT AGC^GCT EcoO65I 17 GGTGACC G^GT[ACGT]ACC EcoRV 2818 GATATC GAT^ATC FseI 3869 GGCCGGCC GGCCGG^CC KasI 4060 GGCGCC G^GCGCC KpnI 385 GGTACC GGTAC^C NaeI 3870 GCCGGC GCC^GGC NarI 4060 GGCGCC GG^CGCC NcoI 1020 CCATGG C^CATGG NgoMIV 3870 GCCGGC G^CCGGC NspV 5015 TTCGAA TT^CGAA PaeR7I 903 CTCGAG C^TCGAG PinAI 1255 ACCGGT A^CCGGT PmaCI 3791 CACGTG CAC^GTG PmlI 3791 CACGTG CAC^GTG PshAI 98 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1649 ATTAAT AT^TAAT Psp1406I 2065 AACGTT AA^CGTT PvuII 4661 CAGCTG CAG^CTG SacI 4372 GAGCTC GAGCT^C SalI 5123 GTCGAC G^TCGAC SfiI 1576 GGCCCTAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 4060 GGCGCC GGC^GCC SfuI 5015 TTCGAA TT^CGAA SpeI 1604 ACTAGT A^CTAGT SspBI 1221 TGTACA T^GTACA XcmI 3774 CCAAGAAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 903 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccI 3205 GTCTAC GT^[AC][GT]AC 5123 GTCGAC AccII 2354 CGCG CG^CG 4129 CGCG AhdI 2546 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3197 GACACCCTGTC AspEI 2546 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3197 GACACCCTGTC BsaBI 721 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4965 GATACACATC BsaI 3912 GAGACC GGTCTC(1/5) 3026 GGTCTC BspHI 1026 TCATGA T^CATGA 2766 TCATGA BstAPI 638 GCAGACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1288 GCAACCTCTGC BstUI 2354 CGCG CG^CG 4129 CGCG DrdI 899 GACCCTCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5126 GACCCAAGTGTC Eam1105I 2546 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3197 GACACCCTGTC EcoNI 4150 CCTTCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4216 CCTTCCCTAGG HaeII 3988 AGCGCT [GA]GCGC^[CT] 4060 GGCGCC HpaI 4782 GTTAAC GTT^AAC 4940 GTTAAC MamI 721 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4965 GATACACATC MvnI 2354 CGCG CG^CG 4129 CGCG RcaI 1026 TCATGA T^CATGA 2766 TCATGA ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccIII AciI AcyI AfaI AluI AlwI AlwNI Asp700I AspHI AvaI AvaII AvrII BalI BamHI BanI BanII BbsI BbvI BclI BcnI BfaI BglI BglII BlnI BmyI BpmI BpuAI BsaHI BsaJI BsaWI BseAI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspEI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI