##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AccIII AclI AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI AspEI AviII AvrII BamHI
BbrPI BclI BglI BlnI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BsrFI BssHII BstBI Bsu36I Cfr10I
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRV EcoT22I
FbaI FseI FspI HpaI KspI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AatII 3864 GACGTC GACGT^C
Acc65I 5921 GGTACC G^GTACC
AccII 240 CGCG CG^CG
Asp718I 5921 GGTACC G^GTACC
BbeI 1652 GGCGCC GGCGC^C
BbvCI 5212 CCTCAGC CCTCAGC(-5/-2)
BcgI 949 GCAGCCTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 3350 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 5251 CGTCTC CGTCTC(1/5)
Bsp1407I 5604 TGTACA T^GTACA
BsrBI 3848 GAGCGG CCGCTC(-3/-3)
BsrGI 5604 TGTACA T^GTACA
BssSI 666 CACGAG CACGAG(-5/-1)
BstUI 240 CGCG CG^CG
DraI 3520 TTTAAA TTT^AAA
DsaI 6032 CCATGG C^C[GA][CT]GG
KasI 1652 GGCGCC G^GCGCC
KpnI 5921 GGTACC GGTAC^C
MamI 3350 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 240 CGCG CG^CG
NarI 1652 GGCGCC GG^CGCC
NcoI 6032 CCATGG C^CATGG
SexAI 2447 ACCTGGT A^CC[AT]GGT
SfoI 1652 GGCGCC GGC^GCC
SphI 1298 GCATGC GCATG^C
SspBI 5604 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 1652 GGCGCC G[GA]^CG[CT]C
3864 GACGTC
ApaI 2034 GGGCCC GGGCC^C
4026 GGGCCC
Asp700I 47 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1014 GAAATGCTTC
AspI 3460 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3837 GACTGAGTC
BciVI 4609 GGATAC GTATCC(6/5)
522 GTATCC
BsaAI 3624 CACGTA [CT]AC^GT[GA]
5867 TACGTG
BsaHI 1652 GGCGCC G[GA]^CG[CT]C
3864 GACGTC
BsgI 4360 CTGCAC GTGCAG(16/14)
275 GTGCAG
BspHI 4774 TCATGA T^CATGA
5358 TCATGA
BsrDI 4479 CATTGC GCAATG(2/0)
2657 GCAATG
Bst1107I 1828 GTATAC GTA^TAC
4240 GTATAC
BstEII 1487 GGTGACC G^GT[ACGT]ACC
2171 GGTCACC
BstPI 1487 GGTGACC G^GT[ACGT]ACC
2171 GGTCACC
BstZ17I 1828 GTATAC GTA^TAC
4240 GTATAC
DrdI 2163 GACACCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4154 GACAGCACTGTC
EcoO65I 1487 GGTGACC G^GT[ACGT]ACC
2171 GGTCACC
EcoRI 2213 GAATTC G^AATTC
2270 GAATTC
FauI 181 CCCGC CCCGC(4/6)
768 CCCGC
HgaI 882 GACGC GACGC(5/10)
1538 GACGC
Hin1I 1652 GGCGCC G[GA]^CG[CT]C
3864 GACGTC
NheI 600 GCTAGC G^CTAGC
6050 GCTAGC
PaeR7I 957 CTCGAG C^TCGAG
2982 CTCGAG
PflFI 3460 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3837 GACTGAGTC
PshAI 1190 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4212 GACTCTTGTC
PvuII 42 CAGCTG CAG^CTG
2293 CAGCTG
RcaI 4774 TCATGA T^CATGA
5358 TCATGA
SapI 2520 GAAGAGC GCTCTTC(1/4)
4286 GCTCTTC
ScaI 4414 AGTACT AGT^ACT
5888 AGTACT
SmaI 449 CCCGGG CCC^GGG
1935 CCCGGG
StuI 694 AGGCCT AGG^CCT
4669 AGGCCT
Tth111I 3460 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3837 GACTGAGTC
XhoI 957 CTCGAG C^TCGAG
2982 CTCGAG
XmaCI 449 CCCGGG C^CCGGG
1935 CCCGGG
XmaI 449 CCCGGG C^CCGGG
1935 CCCGGG
XmnI 47 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1014 GAAATGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BfrI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspLU11I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII