################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AccIII AclI AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AspEI AviII AvrII BamHI BbrPI BclI BglI BlnI BsaWI BseAI BsiEI BsiWI BspDI BspEI BsrFI BssHII BstBI Bsu36I Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FbaI FseI FspI HpaI KspI MfeI MluI MroI MunI NaeI NdeI NgoMIV NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SwaI XbaI ################################### # ONE CUT ENZYMES ( 26 enzymes) # ################################### AatII 3864 GACGTC GACGT^C Acc65I 5921 GGTACC G^GTACC AccII 240 CGCG CG^CG Asp718I 5921 GGTACC G^GTACC BbeI 1652 GGCGCC GGCGC^C BbvCI 5212 CCTCAGC CCTCAGC(-5/-2) BcgI 949 GCAGCCTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaBI 3350 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 5251 CGTCTC CGTCTC(1/5) Bsp1407I 5604 TGTACA T^GTACA BsrBI 3848 GAGCGG CCGCTC(-3/-3) BsrGI 5604 TGTACA T^GTACA BssSI 666 CACGAG CACGAG(-5/-1) BstUI 240 CGCG CG^CG DraI 3520 TTTAAA TTT^AAA DsaI 6032 CCATGG C^C[GA][CT]GG KasI 1652 GGCGCC G^GCGCC KpnI 5921 GGTACC GGTAC^C MamI 3350 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 240 CGCG CG^CG NarI 1652 GGCGCC GG^CGCC NcoI 6032 CCATGG C^CATGG SexAI 2447 ACCTGGT A^CC[AT]GGT SfoI 1652 GGCGCC GGC^GCC SphI 1298 GCATGC GCATG^C SspBI 5604 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AcyI 1652 GGCGCC G[GA]^CG[CT]C 3864 GACGTC ApaI 2034 GGGCCC GGGCC^C 4026 GGGCCC Asp700I 47 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1014 GAAATGCTTC AspI 3460 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3837 GACTGAGTC BciVI 4609 GGATAC GTATCC(6/5) 522 GTATCC BsaAI 3624 CACGTA [CT]AC^GT[GA] 5867 TACGTG BsaHI 1652 GGCGCC G[GA]^CG[CT]C 3864 GACGTC BsgI 4360 CTGCAC GTGCAG(16/14) 275 GTGCAG BspHI 4774 TCATGA T^CATGA 5358 TCATGA BsrDI 4479 CATTGC GCAATG(2/0) 2657 GCAATG Bst1107I 1828 GTATAC GTA^TAC 4240 GTATAC BstEII 1487 GGTGACC G^GT[ACGT]ACC 2171 GGTCACC BstPI 1487 GGTGACC G^GT[ACGT]ACC 2171 GGTCACC BstZ17I 1828 GTATAC GTA^TAC 4240 GTATAC DrdI 2163 GACACCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4154 GACAGCACTGTC EcoO65I 1487 GGTGACC G^GT[ACGT]ACC 2171 GGTCACC EcoRI 2213 GAATTC G^AATTC 2270 GAATTC FauI 181 CCCGC CCCGC(4/6) 768 CCCGC HgaI 882 GACGC GACGC(5/10) 1538 GACGC Hin1I 1652 GGCGCC G[GA]^CG[CT]C 3864 GACGTC NheI 600 GCTAGC G^CTAGC 6050 GCTAGC PaeR7I 957 CTCGAG C^TCGAG 2982 CTCGAG PflFI 3460 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3837 GACTGAGTC PshAI 1190 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4212 GACTCTTGTC PvuII 42 CAGCTG CAG^CTG 2293 CAGCTG RcaI 4774 TCATGA T^CATGA 5358 TCATGA SapI 2520 GAAGAGC GCTCTTC(1/4) 4286 GCTCTTC ScaI 4414 AGTACT AGT^ACT 5888 AGTACT SmaI 449 CCCGGG CCC^GGG 1935 CCCGGG StuI 694 AGGCCT AGG^CCT 4669 AGGCCT Tth111I 3460 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3837 GACTGAGTC XhoI 957 CTCGAG C^TCGAG 2982 CTCGAG XmaCI 449 CCCGGG C^CCGGG 1935 CCCGGG XmaI 449 CCCGGG C^CCGGG 1935 CCCGGG XmnI 47 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1014 GAAATGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccI AciI AcsI AfaI AflII AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BfrI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BspMI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII