##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
AflII AscI AseI AsnI BfrI
BsaBI BsiWI Bsp1407I BspDI BspLU11I
BsrBI BsrGI Bst1107I BstBI BstZ17I
ClaI CpoI EcoT22I HindIII HpaI
MamI MfeI MluI MunI NotI
NsiI NspV PacI PmeI PshBI
PvuI RsrII SalI SbfI SexAI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SwaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 2753 GACGTC GACGT^C
AflIII 5590 ACACGT A^C[GA][CT]GT
AgeI 3317 ACCGGT A^CCGGT
AhdI 2735 GACGCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5493 GTGCAC G^TGCAC
ApaLI 5493 GTGCAC G^TGCAC
AspEI 2735 GACGCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1414 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1736 TGCGCA TGC^GCA
BamHI 3027 GGATCC G^GATCC
BglII 1369 AGATCT A^GATCT
BspHI 3707 TCATGA T^CATGA
BssHII 2553 GCGCGC G^CGCGC
BstEII 5375 GGTGACC G^GT[ACGT]ACC
BstPI 5375 GGTGACC G^GT[ACGT]ACC
BstXI 3911 CCAGGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 5639 TTTAAA TTT^AAA
Eam1105I 2735 GACGCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 5375 GGTGACC G^GT[ACGT]ACC
FseI 2577 GGCCGGCC GGCCGG^CC
FspI 1736 TGCGCA TGC^GCA
NdeI 3428 CATATG CA^TATG
NheI 805 GCTAGC G^CTAGC
NruI 429 TCGCGA TCG^CGA
PaeR7I 2094 CTCGAG C^TCGAG
PflFI 1414 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3317 ACCGGT A^CCGGT
PshAI 3053 GACCTAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3707 TCATGA T^CATGA
SapI 3754 GCTCTTC GCTCTTC(1/4)
ScaI 1666 AGTACT AGT^ACT
SfiI 2868 GGCCGGGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2239 CGCCGGCG C[GA]^CCGG[CT]G
SspI 1234 AATATT AAT^ATT
Tth111I 1414 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1564 TCTAGA T^CTAGA
XhoI 2094 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 645 GGTACC G^GTACC
1960 GGTACC
AccI 709 GTCTAC GT^[AC][GT]AC
1411 GTAGAC
AcsI 3905 GAATTC [GA]^AATT[CT]
5753 GAATTC
ApoI 3905 GAATTC [GA]^AATT[CT]
5753 GAATTC
Asp718I 645 GGTACC G^GTACC
1960 GGTACC
AvrII 3055 CCTAGG C^CTAGG
3223 CCTAGG
BciVI 736 GGATAC GTATCC(6/5)
5461 GTATCC
BclI 459 TGATCA T^GATCA
4652 TGATCA
BlnI 3055 CCTAGG C^CTAGG
3223 CCTAGG
BsmBI 2957 CGTCTC CGTCTC(1/5)
4304 GAGACG
BsmI 3242 GAATGC GAATGC(1/-1)
284 GCATTC
BssSI 4852 CACGAG CACGAG(-5/-1)
1938 CTCGTG
BstAPI 2414 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5201 GCAAAAGCTGC
DraIII 2194 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
4957 CACTTCGTG
DrdI 2764 GACCCTCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4714 GACAAGGTGGTC
EcoRI 3905 GAATTC G^AATTC
5753 GAATTC
EcoRV 4183 GATATC GAT^ATC
4909 GATATC
FbaI 459 TGATCA T^GATCA
4652 TGATCA
KpnI 645 GGTACC GGTAC^C
1960 GGTACC
MslI 93 CATGGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1069 CACGTGGGTG
NcoI 92 CCATGG C^CATGG
3332 CCATGG
NspI 2940 GCATGT [GA]CATG^[CT]
4484 ACATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 157 enzymes) #
#########################################
AccII AccIII AciI AclI AcyI
AfaI AluI AlwI AlwNI AocI
Aor51HI ApaI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspEI
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PmaCI
PmlI PpuMI Psp1406I PstI PvuII
RsaI SacI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI