##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
AscI Asp718I AspEI AviII BamHI
BclI BfrI BsiWI BspDI BspHI
Bst1107I BstBI BstZ17I ClaI Eam1105I
EcoRI EcoT22I FbaI FseI FspI
HpaI KpnI MfeI MunI NcoI
NotI NruI NsiI NspV PacI
PinAI PmeI Psp1406I RcaI SalI
ScaI SexAI SfiI SfuI SgrAI
SnaBI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 3458 GACGTC GACGT^C
Aor51HI 123 AGCGCT AGC^GCT
ApaI 275 GGGCCC GGGCC^C
AseI 350 ATTAAT AT^TAAT
AsnI 350 ATTAAT AT^TAAT
Asp700I 4659 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 4421 CCTAGG C^CTAGG
BbeI 52 GGCGCC GGCGC^C
BcgI 3735 CGAGCAGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1066 GGATAC GTATCC(6/5)
BglI 2911 GCCAGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4421 CCTAGG C^CTAGG
BsaBI 4648 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 4266 TGTACA T^GTACA
BspLU11I 6347 ACATGT A^CATGT
BsrBI 1374 GAGCGG CCGCTC(-3/-3)
BsrFI 86 GCCGGC [GA]^CCGG[CT]
BsrGI 4266 TGTACA T^GTACA
BssHII 4381 GCGCGC G^CGCGC
BstXI 1910 CCAAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 86 GCCGGC [GA]^CCGG[CT]
CpoI 975 CGGACCG CG^G[AT]CCG
DraIII 655 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1269 GACCAAGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 23 CCGCGG C^C[GA][CT]GG
EagI 84 CGGCCG C^GGCCG
EclXI 84 CGGCCG C^GGCCG
Eco47III 123 AGCGCT AGC^GCT
Eco52I 84 CGGCCG C^GGCCG
EcoRV 3783 GATATC GAT^ATC
KasI 52 GGCGCC G^GCGCC
KspI 23 CCGCGG CCGC^GG
MamI 4648 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 6012 ACGCGT A^CGCGT
NaeI 86 GCCGGC GCC^GGC
NarI 52 GGCGCC GG^CGCC
NdeI 6554 CATATG CA^TATG
NgoMIV 86 GCCGGC G^CCGGC
NheI 1227 GCTAGC G^CTAGC
PshBI 350 ATTAAT AT^TAAT
PvuI 3746 CGATCG CGAT^CG
RsrII 975 CGGACCG CG^G[AT]CCG
SacII 23 CCGCGG CCGC^GG
SfoI 52 GGCGCC GGC^GCC
SpeI 4532 ACTAGT A^CTAGT
SspBI 4266 TGTACA T^GTACA
SspI 6374 AATATT AAT^ATT
StuI 4424 AGGCCT AGG^CCT
SwaI 6699 ATTTAAAT ATTT^AAAT
XmnI 4659 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 3963 GTCTAC GT^[AC][GT]AC
6430 GTAGAC
AccIII 76 TCCGGA T^CCGGA
4367 TCCGGA
Alw44I 2124 GTGCAC G^TGCAC
2732 GTGCAC
AocI 1394 CCTCAGG CC^T[ACGT]AGG
5996 CCTGAGG
ApaLI 2124 GTGCAC G^TGCAC
2732 GTGCAC
BalI 2930 TGGCCA TGG^CCA
3019 TGGCCA
BbrPI 252 CACGTG CAC^GTG
4610 CACGTG
BseAI 76 TCCGGA T^CCGGA
4367 TCCGGA
BsmBI 3912 GAGACG CGTCTC(1/5)
4008 GAGACG
BspEI 76 TCCGGA T^CCGGA
4367 TCCGGA
BsrDI 993 GCAATG GCAATG(2/0)
4587 GCAATG
Bsu36I 1394 CCTCAGG CC^T[ACGT]AGG
5996 CCTGAGG
Eco81I 1394 CCTCAGG CC^T[ACGT]AGG
5996 CCTGAGG
MluNI 2930 TGGCCA TGG^CCA
3019 TGGCCA
MroI 76 TCCGGA T^CCGGA
4367 TCCGGA
MscI 2930 TGGCCA TGG^CCA
3019 TGGCCA
PmaCI 252 CACGTG CAC^GTG
4610 CACGTG
PmlI 252 CACGTG CAC^GTG
4610 CACGTG
SbfI 4124 CCTGCAGG CCTGCA^GG
6137 CCTGCAGG
Sse8387I 4124 CCTGCAGG CCTGCA^GG
6137 CCTGCAGG
XbaI 366 TCTAGA T^CTAGA
2462 TCTAGA
XcmI 5875 CCAGCAGGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6021 CCATCCTCTCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AspI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrI
BssKI BssSI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PaeR7I PflFI PflMI PleI
PpuMI PshAI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XhoI XhoII XmaCI XmaI