##################################
# NO CUT ENZYMES (   46 enzymes) #
##################################

Acc65I      AclI        AflII       AgeI        AhdI        
AscI        Asp718I     AspEI       AviII       BamHI       
BclI        BfrI        BsiWI       BspDI       BspHI       
Bst1107I    BstBI       BstZ17I     ClaI        Eam1105I    
EcoRI       EcoT22I     FbaI        FseI        FspI        
HpaI        KpnI        MfeI        MunI        NcoI        
NotI        NruI        NsiI        NspV        PacI        
PinAI       PmeI        Psp1406I    RcaI        SalI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SnaBI       


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AatII        3458  GACGTC               GACGT^C
Aor51HI       123  AGCGCT               AGC^GCT
ApaI          275  GGGCCC               GGGCC^C
AseI          350  ATTAAT               AT^TAAT
AsnI          350  ATTAAT               AT^TAAT
Asp700I      4659  GAAAAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII        4421  CCTAGG               C^CTAGG
BbeI           52  GGCGCC               GGCGC^C
BcgI         3735  CGAGCAGACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1066  GGATAC               GTATCC(6/5)
BglI         2911  GCCAGCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         4421  CCTAGG               C^CTAGG
BsaBI        4648  GATATGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I     4266  TGTACA               T^GTACA
BspLU11I     6347  ACATGT               A^CATGT
BsrBI        1374  GAGCGG               CCGCTC(-3/-3)
BsrFI          86  GCCGGC               [GA]^CCGG[CT]
BsrGI        4266  TGTACA               T^GTACA
BssHII       4381  GCGCGC               G^CGCGC
BstXI        1910  CCAAGGGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I         86  GCCGGC               [GA]^CCGG[CT]
CpoI          975  CGGACCG              CG^G[AT]CCG
DraIII        655  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1269  GACCAAGTAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI           23  CCGCGG               C^C[GA][CT]GG
EagI           84  CGGCCG               C^GGCCG
EclXI          84  CGGCCG               C^GGCCG
Eco47III      123  AGCGCT               AGC^GCT
Eco52I         84  CGGCCG               C^GGCCG
EcoRV        3783  GATATC               GAT^ATC
KasI           52  GGCGCC               G^GCGCC
KspI           23  CCGCGG               CCGC^GG
MamI         4648  GATATGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         6012  ACGCGT               A^CGCGT
NaeI           86  GCCGGC               GCC^GGC
NarI           52  GGCGCC               GG^CGCC
NdeI         6554  CATATG               CA^TATG
NgoMIV         86  GCCGGC               G^CCGGC
NheI         1227  GCTAGC               G^CTAGC
PshBI         350  ATTAAT               AT^TAAT
PvuI         3746  CGATCG               CGAT^CG
RsrII         975  CGGACCG              CG^G[AT]CCG
SacII          23  CCGCGG               CCGC^GG
SfoI           52  GGCGCC               GGC^GCC
SpeI         4532  ACTAGT               A^CTAGT
SspBI        4266  TGTACA               T^GTACA
SspI         6374  AATATT               AAT^ATT
StuI         4424  AGGCCT               AGG^CCT
SwaI         6699  ATTTAAAT             ATTT^AAAT
XmnI         4659  GAAAAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccI         3963  GTCTAC               GT^[AC][GT]AC
             6430  GTAGAC              
AccIII         76  TCCGGA               T^CCGGA
             4367  TCCGGA              
Alw44I       2124  GTGCAC               G^TGCAC
             2732  GTGCAC              
AocI         1394  CCTCAGG              CC^T[ACGT]AGG
             5996  CCTGAGG             
ApaLI        2124  GTGCAC               G^TGCAC
             2732  GTGCAC              
BalI         2930  TGGCCA               TGG^CCA
             3019  TGGCCA              
BbrPI         252  CACGTG               CAC^GTG
             4610  CACGTG              
BseAI          76  TCCGGA               T^CCGGA
             4367  TCCGGA              
BsmBI        3912  GAGACG               CGTCTC(1/5)
             4008  GAGACG              
BspEI          76  TCCGGA               T^CCGGA
             4367  TCCGGA              
BsrDI         993  GCAATG               GCAATG(2/0)
             4587  GCAATG              
Bsu36I       1394  CCTCAGG              CC^T[ACGT]AGG
             5996  CCTGAGG             
Eco81I       1394  CCTCAGG              CC^T[ACGT]AGG
             5996  CCTGAGG             
MluNI        2930  TGGCCA               TGG^CCA
             3019  TGGCCA              
MroI           76  TCCGGA               T^CCGGA
             4367  TCCGGA              
MscI         2930  TGGCCA               TGG^CCA
             3019  TGGCCA              
PmaCI         252  CACGTG               CAC^GTG
             4610  CACGTG              
PmlI          252  CACGTG               CAC^GTG
             4610  CACGTG              
SbfI         4124  CCTGCAGG             CCTGCA^GG
             6137  CCTGCAGG            
Sse8387I     4124  CCTGCAGG             CCTGCA^GG
             6137  CCTGCAGG            
XbaI          366  TCTAGA               T^CTAGA
             2462  TCTAGA              
XcmI         5875  CCAGCAGGAAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             6021  CCATCCTCTCACTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  140 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       ApoI        
AspHI       AspI        AvaI        AvaII       BanI        
BanII       BbsI        BbvCI       BbvI        BcnI        
BfaI        BglII       BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    BspMI       BsrI        
BssKI       BssSI       BstAPI      BstEII      BstF5I      
BstNI       BstPI       BstUI       BstYI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       EaeI        EarI        EcoNI       
EcoO109I    EcoO65I     EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PaeR7I      PflFI       PflMI       PleI        
PpuMI       PshAI       PstI        PvuII       RsaI        
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        SmlI        SphI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Tth111I     TthHB8I     
Van91I      XhoI        XhoII       XmaCI       XmaI