################################## # NO CUT ENZYMES ( 46 enzymes) # ################################## Acc65I AclI AflII AgeI AhdI AscI Asp718I AspEI AviII BamHI BclI BfrI BsiWI BspDI BspHI Bst1107I BstBI BstZ17I ClaI Eam1105I EcoRI EcoT22I FbaI FseI FspI HpaI KpnI MfeI MunI NcoI NotI NruI NsiI NspV PacI PinAI PmeI Psp1406I RcaI SalI ScaI SexAI SfiI SfuI SgrAI SnaBI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 3458 GACGTC GACGT^C Aor51HI 123 AGCGCT AGC^GCT ApaI 275 GGGCCC GGGCC^C AseI 350 ATTAAT AT^TAAT AsnI 350 ATTAAT AT^TAAT Asp700I 4659 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 4421 CCTAGG C^CTAGG BbeI 52 GGCGCC GGCGC^C BcgI 3735 CGAGCAGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1066 GGATAC GTATCC(6/5) BglI 2911 GCCAGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 4421 CCTAGG C^CTAGG BsaBI 4648 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC Bsp1407I 4266 TGTACA T^GTACA BspLU11I 6347 ACATGT A^CATGT BsrBI 1374 GAGCGG CCGCTC(-3/-3) BsrFI 86 GCCGGC [GA]^CCGG[CT] BsrGI 4266 TGTACA T^GTACA BssHII 4381 GCGCGC G^CGCGC BstXI 1910 CCAAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 86 GCCGGC [GA]^CCGG[CT] CpoI 975 CGGACCG CG^G[AT]CCG DraIII 655 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1269 GACCAAGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 23 CCGCGG C^C[GA][CT]GG EagI 84 CGGCCG C^GGCCG EclXI 84 CGGCCG C^GGCCG Eco47III 123 AGCGCT AGC^GCT Eco52I 84 CGGCCG C^GGCCG EcoRV 3783 GATATC GAT^ATC KasI 52 GGCGCC G^GCGCC KspI 23 CCGCGG CCGC^GG MamI 4648 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 6012 ACGCGT A^CGCGT NaeI 86 GCCGGC GCC^GGC NarI 52 GGCGCC GG^CGCC NdeI 6554 CATATG CA^TATG NgoMIV 86 GCCGGC G^CCGGC NheI 1227 GCTAGC G^CTAGC PshBI 350 ATTAAT AT^TAAT PvuI 3746 CGATCG CGAT^CG RsrII 975 CGGACCG CG^G[AT]CCG SacII 23 CCGCGG CCGC^GG SfoI 52 GGCGCC GGC^GCC SpeI 4532 ACTAGT A^CTAGT SspBI 4266 TGTACA T^GTACA SspI 6374 AATATT AAT^ATT StuI 4424 AGGCCT AGG^CCT SwaI 6699 ATTTAAAT ATTT^AAAT XmnI 4659 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 3963 GTCTAC GT^[AC][GT]AC 6430 GTAGAC AccIII 76 TCCGGA T^CCGGA 4367 TCCGGA Alw44I 2124 GTGCAC G^TGCAC 2732 GTGCAC AocI 1394 CCTCAGG CC^T[ACGT]AGG 5996 CCTGAGG ApaLI 2124 GTGCAC G^TGCAC 2732 GTGCAC BalI 2930 TGGCCA TGG^CCA 3019 TGGCCA BbrPI 252 CACGTG CAC^GTG 4610 CACGTG BseAI 76 TCCGGA T^CCGGA 4367 TCCGGA BsmBI 3912 GAGACG CGTCTC(1/5) 4008 GAGACG BspEI 76 TCCGGA T^CCGGA 4367 TCCGGA BsrDI 993 GCAATG GCAATG(2/0) 4587 GCAATG Bsu36I 1394 CCTCAGG CC^T[ACGT]AGG 5996 CCTGAGG Eco81I 1394 CCTCAGG CC^T[ACGT]AGG 5996 CCTGAGG MluNI 2930 TGGCCA TGG^CCA 3019 TGGCCA MroI 76 TCCGGA T^CCGGA 4367 TCCGGA MscI 2930 TGGCCA TGG^CCA 3019 TGGCCA PmaCI 252 CACGTG CAC^GTG 4610 CACGTG PmlI 252 CACGTG CAC^GTG 4610 CACGTG SbfI 4124 CCTGCAGG CCTGCA^GG 6137 CCTGCAGG Sse8387I 4124 CCTGCAGG CCTGCA^GG 6137 CCTGCAGG XbaI 366 TCTAGA T^CTAGA 2462 TCTAGA XcmI 5875 CCAGCAGGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 6021 CCATCCTCTCACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 140 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AspI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PaeR7I PflFI PflMI PleI PpuMI PshAI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XhoI XhoII XmaCI XmaI