##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AcsI AcyI ApoI AscI
AseI AsnI AspI BbeI BsaBI
BsaHI BsiEI BsiWI BsmBI BspDI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI EagI EclXI Eco52I
EcoRI EcoRV Hin1I HpaI KasI
MamI MfeI MunI NarI NdeI
NotI NruI NspV PacI PflFI
PmeI PshAI PshBI PvuI RsrII
SbfI SfoI SfuI SnaBI SphI
Sse8387I SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AclI 878 AACGTT AA^CGTT
AflII 519 CTTAAG C^TTAAG
AgeI 3646 ACCGGT A^CCGGT
AhdI 5021 GACACTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 407 AGCGCT AGC^GCT
Asp700I 3714 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 5021 GACACTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2993 TGCGCA TGC^GCA
AvrII 300 CCTAGG C^CTAGG
BamHI 2619 GGATCC G^GATCC
BfrI 519 CTTAAG C^TTAAG
BglII 6135 AGATCT A^GATCT
BlnI 300 CCTAGG C^CTAGG
BlpI 1140 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1140 GCTCAGC GC^T[ACGT]AGC
BspLU11I 3365 ACATGT A^CATGT
CelII 1140 GCTCAGC GC^T[ACGT]AGC
Eam1105I 5021 GACACTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 407 AGCGCT AGC^GCT
EcoT22I 4838 ATGCAT ATGCA^T
FseI 90 GGCCGGCC GGCCGG^CC
FspI 2993 TGCGCA TGC^GCA
HindIII 5617 AAGCTT A^AGCTT
KspI 1328 CCGCGG CCGC^GG
MluI 1820 ACGCGT A^CGCGT
NaeI 91 GCCGGC GCC^GGC
NgoMIV 91 GCCGGC G^CCGGC
NsiI 4838 ATGCAT ATGCA^T
PinAI 3646 ACCGGT A^CCGGT
Psp1406I 878 AACGTT AA^CGTT
SacII 1328 CCGCGG CCGC^GG
SalI 4778 GTCGAC G^TCGAC
ScaI 6128 AGTACT AGT^ACT
SgrAI 3645 CACCGGTG C[GA]^CCGG[CT]G
SpeI 4509 ACTAGT A^CTAGT
TfiI 3518 GATTC G^A[AT]TC
XmnI 3714 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 1753 GGTACC G^GTACC
4600 GGTACC
AccIII 1388 TCCGGA T^CCGGA
4090 TCCGGA
Alw44I 584 GTGCAC G^TGCAC
3253 GTGCAC
AocI 4368 CCTGAGG CC^T[ACGT]AGG
5900 CCTCAGG
ApaLI 584 GTGCAC G^TGCAC
3253 GTGCAC
Asp718I 1753 GGTACC G^GTACC
4600 GGTACC
BbvCI 2030 GCTGAGG CCTCAGC(-5/-2)
2300 GCTGAGG
BclI 5651 TGATCA T^GATCA
5828 TGATCA
BseAI 1388 TCCGGA T^CCGGA
4090 TCCGGA
BsmI 1356 GAATGC GAATGC(1/-1)
5123 GAATGC
BspEI 1388 TCCGGA T^CCGGA
4090 TCCGGA
BsrFI 91 GCCGGC [GA]^CCGG[CT]
3646 ACCGGT
BstAPI 1073 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1176 GCACCAGGTGC
BstEII 3649 GGTGACC G^GT[ACGT]ACC
5604 GGTGACC
BstPI 3649 GGTGACC G^GT[ACGT]ACC
5604 GGTGACC
Bsu36I 4368 CCTGAGG CC^T[ACGT]AGG
5900 CCTCAGG
Cfr10I 91 GCCGGC [GA]^CCGG[CT]
3646 ACCGGT
DrdI 508 GACGGTTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3457 GACGATTTTGTC
Eco81I 4368 CCTGAGG CC^T[ACGT]AGG
5900 CCTCAGG
EcoO65I 3649 GGTGACC G^GT[ACGT]ACC
5604 GGTGACC
FbaI 5651 TGATCA T^GATCA
5828 TGATCA
HincII 4778 GTCGAC GT[CT]^[GA]AC
5845 GTCAAC
HindII 4778 GTCGAC GT[CT]^[GA]AC
5845 GTCAAC
KpnI 1753 GGTACC GGTAC^C
4600 GGTACC
MroI 1388 TCCGGA T^CCGGA
4090 TCCGGA
NheI 175 GCTAGC G^CTAGC
2656 GCTAGC
PaeR7I 1030 CTCGAG C^TCGAG
1264 CTCGAG
SacI 5425 GAGCTC GAGCT^C
5791 GAGCTC
SapI 3840 GCTCTTC GCTCTTC(1/4)
4080 GCTCTTC
SexAI 1178 ACCAGGT A^CC[AT]GGT
4358 ACCAGGT
SfiI 1855 GGCCGAACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2834 GGCCTGCAAGGCC
SmaI 1134 CCCGGG CCC^GGG
2022 CCCGGG
XbaI 1437 TCTAGA T^CTAGA
2935 TCTAGA
XhoI 1030 CTCGAG C^TCGAG
1264 CTCGAG
XmaCI 1134 CCCGGG C^CCGGG
2022 CCCGGG
XmaI 1134 CCCGGG C^CCGGG
2022 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AccII AciI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbsI BbvI BcgI BciVI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspHI BspMI BsrBI BsrDI BsrGI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII