################################## # NO CUT ENZYMES ( 54 enzymes) # ################################## AatII AcsI AcyI ApoI AscI AseI AsnI AspI BbeI BsaBI BsaHI BsiEI BsiWI BsmBI BspDI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI EagI EclXI Eco52I EcoRI EcoRV Hin1I HpaI KasI MamI MfeI MunI NarI NdeI NotI NruI NspV PacI PflFI PmeI PshAI PshBI PvuI RsrII SbfI SfoI SfuI SnaBI SphI Sse8387I SspI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AclI 878 AACGTT AA^CGTT AflII 519 CTTAAG C^TTAAG AgeI 3646 ACCGGT A^CCGGT AhdI 5021 GACACTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 407 AGCGCT AGC^GCT Asp700I 3714 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 5021 GACACTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2993 TGCGCA TGC^GCA AvrII 300 CCTAGG C^CTAGG BamHI 2619 GGATCC G^GATCC BfrI 519 CTTAAG C^TTAAG BglII 6135 AGATCT A^GATCT BlnI 300 CCTAGG C^CTAGG BlpI 1140 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1140 GCTCAGC GC^T[ACGT]AGC BspLU11I 3365 ACATGT A^CATGT CelII 1140 GCTCAGC GC^T[ACGT]AGC Eam1105I 5021 GACACTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 407 AGCGCT AGC^GCT EcoT22I 4838 ATGCAT ATGCA^T FseI 90 GGCCGGCC GGCCGG^CC FspI 2993 TGCGCA TGC^GCA HindIII 5617 AAGCTT A^AGCTT KspI 1328 CCGCGG CCGC^GG MluI 1820 ACGCGT A^CGCGT NaeI 91 GCCGGC GCC^GGC NgoMIV 91 GCCGGC G^CCGGC NsiI 4838 ATGCAT ATGCA^T PinAI 3646 ACCGGT A^CCGGT Psp1406I 878 AACGTT AA^CGTT SacII 1328 CCGCGG CCGC^GG SalI 4778 GTCGAC G^TCGAC ScaI 6128 AGTACT AGT^ACT SgrAI 3645 CACCGGTG C[GA]^CCGG[CT]G SpeI 4509 ACTAGT A^CTAGT TfiI 3518 GATTC G^A[AT]TC XmnI 3714 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 1753 GGTACC G^GTACC 4600 GGTACC AccIII 1388 TCCGGA T^CCGGA 4090 TCCGGA Alw44I 584 GTGCAC G^TGCAC 3253 GTGCAC AocI 4368 CCTGAGG CC^T[ACGT]AGG 5900 CCTCAGG ApaLI 584 GTGCAC G^TGCAC 3253 GTGCAC Asp718I 1753 GGTACC G^GTACC 4600 GGTACC BbvCI 2030 GCTGAGG CCTCAGC(-5/-2) 2300 GCTGAGG BclI 5651 TGATCA T^GATCA 5828 TGATCA BseAI 1388 TCCGGA T^CCGGA 4090 TCCGGA BsmI 1356 GAATGC GAATGC(1/-1) 5123 GAATGC BspEI 1388 TCCGGA T^CCGGA 4090 TCCGGA BsrFI 91 GCCGGC [GA]^CCGG[CT] 3646 ACCGGT BstAPI 1073 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1176 GCACCAGGTGC BstEII 3649 GGTGACC G^GT[ACGT]ACC 5604 GGTGACC BstPI 3649 GGTGACC G^GT[ACGT]ACC 5604 GGTGACC Bsu36I 4368 CCTGAGG CC^T[ACGT]AGG 5900 CCTCAGG Cfr10I 91 GCCGGC [GA]^CCGG[CT] 3646 ACCGGT DrdI 508 GACGGTTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3457 GACGATTTTGTC Eco81I 4368 CCTGAGG CC^T[ACGT]AGG 5900 CCTCAGG EcoO65I 3649 GGTGACC G^GT[ACGT]ACC 5604 GGTGACC FbaI 5651 TGATCA T^GATCA 5828 TGATCA HincII 4778 GTCGAC GT[CT]^[GA]AC 5845 GTCAAC HindII 4778 GTCGAC GT[CT]^[GA]AC 5845 GTCAAC KpnI 1753 GGTACC GGTAC^C 4600 GGTACC MroI 1388 TCCGGA T^CCGGA 4090 TCCGGA NheI 175 GCTAGC G^CTAGC 2656 GCTAGC PaeR7I 1030 CTCGAG C^TCGAG 1264 CTCGAG SacI 5425 GAGCTC GAGCT^C 5791 GAGCTC SapI 3840 GCTCTTC GCTCTTC(1/4) 4080 GCTCTTC SexAI 1178 ACCAGGT A^CC[AT]GGT 4358 ACCAGGT SfiI 1855 GGCCGAACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 2834 GGCCTGCAAGGCC SmaI 1134 CCCGGG CCC^GGG 2022 CCCGGG XbaI 1437 TCTAGA T^CTAGA 2935 TCTAGA XhoI 1030 CTCGAG C^TCGAG 1264 CTCGAG XmaCI 1134 CCCGGG C^CCGGG 2022 CCCGGG XmaI 1134 CCCGGG C^CCGGG 2022 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AccI AccII AciI AfaI AflIII AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvI BcgI BciVI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspHI BspMI BsrBI BsrDI BsrGI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII