##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI Alw44I
ApaLI AscI Asp718I BbrPI BcgI
BciVI BclI BfrI BsaAI BsaBI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII Bst1107I
BstUI BstZ17I ClaI CpoI DrdI
EagI EclXI Eco52I EcoRI EcoRV
FbaI FseI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI PacI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI ScaI SexAI SmaI SnaBI
SpeI SspBI SspI SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcsI 1863 GAATTT [GA]^AATT[CT]
ApaI 2625 GGGCCC GGGCC^C
ApoI 1863 GAATTT [GA]^AATT[CT]
AseI 3894 ATTAAT AT^TAAT
AsnI 3894 ATTAAT AT^TAAT
Asp700I 2415 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 170 CTCGAG C^[CT]CG[GA]G
AviII 838 TGCGCA TGC^GCA
AvrII 1610 CCTAGG C^CTAGG
BamHI 4635 GGATCC G^GATCC
BbeI 1380 GGCGCC GGCGC^C
BbvCI 1924 CCTCAGC CCTCAGC(-5/-2)
BlnI 1610 CCTAGG C^CTAGG
BsmBI 2333 CGTCTC CGTCTC(1/5)
BsoBI 170 CTCGAG C^[CT]CG[GA]G
BspHI 3184 TCATGA T^CATGA
BsrBI 2841 GAGCGG CCGCTC(-3/-3)
BssSI 3121 CTCGTG CACGAG(-5/-1)
BstBI 1885 TTCGAA TT^CGAA
BstEII 2831 GGTCACC G^GT[ACGT]ACC
BstPI 2831 GGTCACC G^GT[ACGT]ACC
DraI 6615 TTTAAA TTT^AAA
DraIII 5332 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 2831 GGTCACC G^GT[ACGT]ACC
EcoT22I 976 ATGCAT ATGCA^T
FspI 838 TGCGCA TGC^GCA
HindIII 2417 AAGCTT A^AGCTT
HpaI 3689 GTTAAC GTT^AAC
KasI 1380 GGCGCC G^GCGCC
NarI 1380 GGCGCC GG^CGCC
NsiI 976 ATGCAT ATGCA^T
NspV 1885 TTCGAA TT^CGAA
PaeR7I 170 CTCGAG C^TCGAG
PshBI 3894 ATTAAT AT^TAAT
RcaI 3184 TCATGA T^CATGA
SbfI 6075 CCTGCAGG CCTGCA^GG
SfiI 3435 GGCCAGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1380 GGCGCC GGC^GCC
SfuI 1885 TTCGAA TT^CGAA
SgrAI 2985 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 6075 CCTGCAGG CCTGCA^GG
XhoI 170 CTCGAG C^TCGAG
XmnI 2415 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 7 enzymes) #
###################################
AocI 3204 CCTGAGG CC^T[ACGT]AGG
4721 CCTGAGG
Aor51HI 3985 AGCGCT AGC^GCT
4420 AGCGCT
BsrDI 858 CATTGC GCAATG(2/0)
1633 GCAATG
Bsu36I 3204 CCTGAGG CC^T[ACGT]AGG
4721 CCTGAGG
Eco47III 3985 AGCGCT AGC^GCT
4420 AGCGCT
Eco81I 3204 CCTGAGG CC^T[ACGT]AGG
4721 CCTGAGG
SacI 384 GAGCTC GAGCT^C
4601 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AciI AcyI AfaI AhdI AluI
AlwI AlwNI AspEI AspHI AspI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eam1105I EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PshAI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoII