##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AflII AgeI AscI AseI
AsnI Asp718I AspI AviII BbeI
BcgI BfrI BglII BsaBI BsiWI
BsmI Bsp1407I BspDI BspLU11I BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI FseI FspI HindIII
KasI KpnI MamI MfeI MluI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PinAI PmeI PshBI PvuI
RsrII SalI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AclI 1001 AACGTT AA^CGTT
AflIII 4483 ACGTGT A^C[GA][CT]GT
Aor51HI 4948 AGCGCT AGC^GCT
AvrII 1220 CCTAGG C^CTAGG
BclI 1144 TGATCA T^GATCA
BglI 2603 GCCTATGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1220 CCTAGG C^CTAGG
BsiEI 982 CGGCCG CG[GA][CT]^CG
BspHI 2398 TCATGA T^CATGA
BsrBI 413 CCGCTC CCGCTC(-3/-3)
BsrDI 1700 CATTGC GCAATG(2/0)
DrdI 2081 GACTGATGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 982 CGGCCG C^GGCCG
EclXI 982 CGGCCG C^GGCCG
Eco47III 4948 AGCGCT AGC^GCT
Eco52I 982 CGGCCG C^GGCCG
EcoRI 3698 GAATTC G^AATTC
EcoRV 681 GATATC GAT^ATC
EcoT22I 5352 ATGCAT ATGCA^T
FbaI 1144 TGATCA T^GATCA
HpaI 4244 GTTAAC GTT^AAC
KspI 50 CCGCGG CCGC^GG
NsiI 5352 ATGCAT ATGCA^T
PaeR7I 241 CTCGAG C^TCGAG
PshAI 5973 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1001 AACGTT AA^CGTT
RcaI 2398 TCATGA T^CATGA
SacII 50 CCGCGG CCGC^GG
SapI 4151 GCTCTTC GCTCTTC(1/4)
ScaI 2901 AGTACT AGT^ACT
XbaI 4302 TCTAGA T^CTAGA
XhoI 241 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BamHI 288 GGATCC G^GATCC
4139 GGATCC
BanI 1764 GGCACC G^G[CT][GA]CC
4554 GGTGCC
BbrPI 2184 CACGTG CAC^GTG
5658 CACGTG
BsaAI 2184 CACGTG [CT]AC^GT[GA]
5658 CACGTG
BsrFI 2236 ACCGGC [GA]^CCGG[CT]
3299 ACCGGC
BssSI 1563 CACGAG CACGAG(-5/-1)
2118 CTCGTG
BstEII 5308 GGTCACC G^GT[ACGT]ACC
5956 GGTCACC
BstPI 5308 GGTCACC G^GT[ACGT]ACC
5956 GGTCACC
BstXI 1754 CCAGTGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4513 CCATAGCTATGG
Cfr10I 2236 ACCGGC [GA]^CCGG[CT]
3299 ACCGGC
DraIII 1785 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
2163 CACAGTGTG
EcoO65I 5308 GGTCACC G^GT[ACGT]ACC
5956 GGTCACC
HaeII 2349 AGCGCC [GA]GCGC^[CT]
4948 AGCGCT
HincII 1593 GTCAAC GT[CT]^[GA]AC
4244 GTTAAC
HindII 1593 GTCAAC GT[CT]^[GA]AC
4244 GTTAAC
PmaCI 2184 CACGTG CAC^GTG
5658 CACGTG
PmlI 2184 CACGTG CAC^GTG
5658 CACGTG
PvuII 1884 CAGCTG CAG^CTG
5869 CAGCTG
SexAI 2218 ACCTGGT A^CC[AT]GGT
5264 ACCAGGT
SmaI 1267 CCCGGG CCC^GGG
3288 CCCGGG
SphI 2460 GCATGC GCATG^C
4021 GCATGC
XmaCI 1267 CCCGGG C^CCGGG
3288 CCCGGG
XmaI 1267 CCCGGG C^CCGGG
3288 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AatII AccI AccII AccIII AciI
AcsI AcyI AfaI AhdI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI ApoI Asp700I AspEI AspHI
AvaI AvaII BalI BanII BbsI
BbvCI BbvI BciVI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI Eam1105I
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI