##################################
# NO CUT ENZYMES (   61 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AscI        AseI        AsnI        AviII       BamHI       
BbeI        BbrPI       BcgI        BciVI       BfrI        
BsaBI       BseAI       BsiWI       BspDI       BspEI       
BstBI       BstEII      BstPI       ClaI        DraI        
EcoO65I     EcoRI       EcoRV       EcoT22I     FseI        
FspI        HindIII     KasI        MamI        MroI        
NarI        NdeI        NotI        NsiI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        SalI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SwaI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AocI         1284  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       316  AGCGCT               AGC^GCT
Asp700I      5761  GAAAAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BsaAI        2672  TACGTG               [CT]AC^GT[GA]
BsiEI        1916  CGGCCG               CG[GA][CT]^CG
BsmBI        1516  CGTCTC               CGTCTC(1/5)
BsmI         3834  GCATTC               GAATGC(1/-1)
Bsp1407I     5904  TGTACA               T^GTACA
BsrGI        5904  TGTACA               T^GTACA
BssHII         64  GCGCGC               G^CGCGC
BstAPI       3318  GCAGCAAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       1284  CCTGAGG              CC^T[ACGT]AGG
CpoI         4975  CGGACCG              CG^G[AT]CCG
EagI         1916  CGGCCG               C^GGCCG
EclXI        1916  CGGCCG               C^GGCCG
Eco47III      316  AGCGCT               AGC^GCT
Eco52I       1916  CGGCCG               C^GGCCG
Eco81I       1284  CCTGAGG              CC^T[ACGT]AGG
HpaI          796  GTTAAC               GTT^AAC
MfeI         2786  CAATTG               C^AATTG
MluI         2551  ACGCGT               A^CGCGT
MunI         2786  CAATTG               C^AATTG
NheI         1479  GCTAGC               G^CTAGC
NruI         1158  TCGCGA               TCG^CGA
PaeR7I        809  CTCGAG               C^TCGAG
RsrII        4975  CGGACCG              CG^G[AT]CCG
SspBI        5904  TGTACA               T^GTACA
SspI         2719  AATATT               AAT^ATT
StuI         5292  AGGCCT               AGG^CCT
XbaI         4450  TCTAGA               T^CTAGA
XhoI          809  CTCGAG               C^TCGAG
XmnI         5761  GAAAAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

Acc65I        407  GGTACC               G^GTACC
             4633  GGTACC              
AcsI         3962  GAATTT               [GA]^AATT[CT]
             5744  GAATTT              
AhdI         2419  GACTTCTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5512  GACTGTGCGTC         
ApaI         1331  GGGCCC               GGGCC^C
             2763  GGGCCC              
ApoI         3962  GAATTT               [GA]^AATT[CT]
             5744  GAATTT              
Asp718I       407  GGTACC               G^GTACC
             4633  GGTACC              
AspEI        2419  GACTTCTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5512  GACTGTGCGTC         
AvrII         945  CCTAGG               C^CTAGG
             5151  CCTAGG              
BglII        3875  AGATCT               A^GATCT
             3908  AGATCT              
BlnI          945  CCTAGG               C^CTAGG
             5151  CCTAGG              
BlpI          999  GCTGAGC              GC^T[ACGT]AGC
             3556  GCTGAGC             
Bpu1102I      999  GCTGAGC              GC^T[ACGT]AGC
             3556  GCTGAGC             
BsaWI        3053  ACCGGA               [AT]^CCGG[AT]
             4973  ACCGGA              
BspHI        3234  TCATGA               T^CATGA
             4008  TCATGA              
BspLU11I     2101  ACATGT               A^CATGT
             3480  ACATGT              
BsrDI        1040  CATTGC               GCAATG(2/0)
             4948  GCAATG              
Bst1107I     2283  GTATAC               GTA^TAC
             3106  GTATAC              
BstXI        2717  CCAATATTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2771  CCAAGTGCATGG        
BstZ17I      2283  GTATAC               GTA^TAC
             3106  GTATAC              
CelII         999  GCTGAGC              GC^T[ACGT]AGC
             3556  GCTGAGC             
DraIII        534  CACAACGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1180  CACACGGTG           
Eam1105I     2419  GACTTCTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5512  GACTGTGCGTC         
HincII        796  GTTAAC               GT[CT]^[GA]AC
             4302  GTTGAC              
HindII        796  GTTAAC               GT[CT]^[GA]AC
             4302  GTTGAC              
KpnI          407  GGTACC               GGTAC^C
             4633  GGTACC              
KspI           39  CCGCGG               CCGC^GG
              207  CCGCGG              
NcoI         1232  CCATGG               C^CATGG
             4171  CCATGG              
RcaI         3234  TCATGA               T^CATGA
             4008  TCATGA              
SacII          39  CCGCGG               CCGC^GG
              207  CCGCGG              
ScaI         1850  AGTACT               AGT^ACT
             3388  AGTACT              
SmaI         2276  CCCGGG               CCC^GGG
             2890  CCCGGG              
XmaCI        2276  CCCGGG               C^CCGGG
             2890  CCCGGG              
XmaI         2276  CCCGGG               C^CCGGG
             2890  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  132 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AflIII      AluI        Alw44I      AlwI        AlwNI       
ApaLI       AspHI       AspI        AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvCI       
BbvI        BclI        BcnI        BfaI        BglI        
BmyI        BpmI        BpuAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BssSI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DrdI        DsaI        EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        FbaI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NciI        NdeII       
NgoMIV      NlaIII      NlaIV       NspI        PflFI       
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SacI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     Van91I      
XcmI        XhoII