##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AscI AseI AsnI AviII BamHI
BbeI BbrPI BcgI BciVI BfrI
BsaBI BseAI BsiWI BspDI BspEI
BstBI BstEII BstPI ClaI DraI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HindIII KasI MamI MroI
NarI NdeI NotI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SwaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AocI 1284 CCTGAGG CC^T[ACGT]AGG
Aor51HI 316 AGCGCT AGC^GCT
Asp700I 5761 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BsaAI 2672 TACGTG [CT]AC^GT[GA]
BsiEI 1916 CGGCCG CG[GA][CT]^CG
BsmBI 1516 CGTCTC CGTCTC(1/5)
BsmI 3834 GCATTC GAATGC(1/-1)
Bsp1407I 5904 TGTACA T^GTACA
BsrGI 5904 TGTACA T^GTACA
BssHII 64 GCGCGC G^CGCGC
BstAPI 3318 GCAGCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1284 CCTGAGG CC^T[ACGT]AGG
CpoI 4975 CGGACCG CG^G[AT]CCG
EagI 1916 CGGCCG C^GGCCG
EclXI 1916 CGGCCG C^GGCCG
Eco47III 316 AGCGCT AGC^GCT
Eco52I 1916 CGGCCG C^GGCCG
Eco81I 1284 CCTGAGG CC^T[ACGT]AGG
HpaI 796 GTTAAC GTT^AAC
MfeI 2786 CAATTG C^AATTG
MluI 2551 ACGCGT A^CGCGT
MunI 2786 CAATTG C^AATTG
NheI 1479 GCTAGC G^CTAGC
NruI 1158 TCGCGA TCG^CGA
PaeR7I 809 CTCGAG C^TCGAG
RsrII 4975 CGGACCG CG^G[AT]CCG
SspBI 5904 TGTACA T^GTACA
SspI 2719 AATATT AAT^ATT
StuI 5292 AGGCCT AGG^CCT
XbaI 4450 TCTAGA T^CTAGA
XhoI 809 CTCGAG C^TCGAG
XmnI 5761 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 407 GGTACC G^GTACC
4633 GGTACC
AcsI 3962 GAATTT [GA]^AATT[CT]
5744 GAATTT
AhdI 2419 GACTTCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5512 GACTGTGCGTC
ApaI 1331 GGGCCC GGGCC^C
2763 GGGCCC
ApoI 3962 GAATTT [GA]^AATT[CT]
5744 GAATTT
Asp718I 407 GGTACC G^GTACC
4633 GGTACC
AspEI 2419 GACTTCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5512 GACTGTGCGTC
AvrII 945 CCTAGG C^CTAGG
5151 CCTAGG
BglII 3875 AGATCT A^GATCT
3908 AGATCT
BlnI 945 CCTAGG C^CTAGG
5151 CCTAGG
BlpI 999 GCTGAGC GC^T[ACGT]AGC
3556 GCTGAGC
Bpu1102I 999 GCTGAGC GC^T[ACGT]AGC
3556 GCTGAGC
BsaWI 3053 ACCGGA [AT]^CCGG[AT]
4973 ACCGGA
BspHI 3234 TCATGA T^CATGA
4008 TCATGA
BspLU11I 2101 ACATGT A^CATGT
3480 ACATGT
BsrDI 1040 CATTGC GCAATG(2/0)
4948 GCAATG
Bst1107I 2283 GTATAC GTA^TAC
3106 GTATAC
BstXI 2717 CCAATATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2771 CCAAGTGCATGG
BstZ17I 2283 GTATAC GTA^TAC
3106 GTATAC
CelII 999 GCTGAGC GC^T[ACGT]AGC
3556 GCTGAGC
DraIII 534 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
1180 CACACGGTG
Eam1105I 2419 GACTTCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5512 GACTGTGCGTC
HincII 796 GTTAAC GT[CT]^[GA]AC
4302 GTTGAC
HindII 796 GTTAAC GT[CT]^[GA]AC
4302 GTTGAC
KpnI 407 GGTACC GGTAC^C
4633 GGTACC
KspI 39 CCGCGG CCGC^GG
207 CCGCGG
NcoI 1232 CCATGG C^CATGG
4171 CCATGG
RcaI 3234 TCATGA T^CATGA
4008 TCATGA
SacII 39 CCGCGG CCGC^GG
207 CCGCGG
ScaI 1850 AGTACT AGT^ACT
3388 AGTACT
SmaI 2276 CCCGGG CCC^GGG
2890 CCCGGG
XmaCI 2276 CCCGGG C^CCGGG
2890 CCCGGG
XmaI 2276 CCCGGG C^CCGGG
2890 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaLI AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BclI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII