################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AatII AccIII AclI AflII AgeI AscI AseI AsnI AviII BamHI BbeI BbrPI BcgI BciVI BfrI BsaBI BseAI BsiWI BspDI BspEI BstBI BstEII BstPI ClaI DraI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HindIII KasI MamI MroI NarI NdeI NotI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AocI 1284 CCTGAGG CC^T[ACGT]AGG Aor51HI 316 AGCGCT AGC^GCT Asp700I 5761 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BsaAI 2672 TACGTG [CT]AC^GT[GA] BsiEI 1916 CGGCCG CG[GA][CT]^CG BsmBI 1516 CGTCTC CGTCTC(1/5) BsmI 3834 GCATTC GAATGC(1/-1) Bsp1407I 5904 TGTACA T^GTACA BsrGI 5904 TGTACA T^GTACA BssHII 64 GCGCGC G^CGCGC BstAPI 3318 GCAGCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1284 CCTGAGG CC^T[ACGT]AGG CpoI 4975 CGGACCG CG^G[AT]CCG EagI 1916 CGGCCG C^GGCCG EclXI 1916 CGGCCG C^GGCCG Eco47III 316 AGCGCT AGC^GCT Eco52I 1916 CGGCCG C^GGCCG Eco81I 1284 CCTGAGG CC^T[ACGT]AGG HpaI 796 GTTAAC GTT^AAC MfeI 2786 CAATTG C^AATTG MluI 2551 ACGCGT A^CGCGT MunI 2786 CAATTG C^AATTG NheI 1479 GCTAGC G^CTAGC NruI 1158 TCGCGA TCG^CGA PaeR7I 809 CTCGAG C^TCGAG RsrII 4975 CGGACCG CG^G[AT]CCG SspBI 5904 TGTACA T^GTACA SspI 2719 AATATT AAT^ATT StuI 5292 AGGCCT AGG^CCT XbaI 4450 TCTAGA T^CTAGA XhoI 809 CTCGAG C^TCGAG XmnI 5761 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 407 GGTACC G^GTACC 4633 GGTACC AcsI 3962 GAATTT [GA]^AATT[CT] 5744 GAATTT AhdI 2419 GACTTCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5512 GACTGTGCGTC ApaI 1331 GGGCCC GGGCC^C 2763 GGGCCC ApoI 3962 GAATTT [GA]^AATT[CT] 5744 GAATTT Asp718I 407 GGTACC G^GTACC 4633 GGTACC AspEI 2419 GACTTCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5512 GACTGTGCGTC AvrII 945 CCTAGG C^CTAGG 5151 CCTAGG BglII 3875 AGATCT A^GATCT 3908 AGATCT BlnI 945 CCTAGG C^CTAGG 5151 CCTAGG BlpI 999 GCTGAGC GC^T[ACGT]AGC 3556 GCTGAGC Bpu1102I 999 GCTGAGC GC^T[ACGT]AGC 3556 GCTGAGC BsaWI 3053 ACCGGA [AT]^CCGG[AT] 4973 ACCGGA BspHI 3234 TCATGA T^CATGA 4008 TCATGA BspLU11I 2101 ACATGT A^CATGT 3480 ACATGT BsrDI 1040 CATTGC GCAATG(2/0) 4948 GCAATG Bst1107I 2283 GTATAC GTA^TAC 3106 GTATAC BstXI 2717 CCAATATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2771 CCAAGTGCATGG BstZ17I 2283 GTATAC GTA^TAC 3106 GTATAC CelII 999 GCTGAGC GC^T[ACGT]AGC 3556 GCTGAGC DraIII 534 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG 1180 CACACGGTG Eam1105I 2419 GACTTCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5512 GACTGTGCGTC HincII 796 GTTAAC GT[CT]^[GA]AC 4302 GTTGAC HindII 796 GTTAAC GT[CT]^[GA]AC 4302 GTTGAC KpnI 407 GGTACC GGTAC^C 4633 GGTACC KspI 39 CCGCGG CCGC^GG 207 CCGCGG NcoI 1232 CCATGG C^CATGG 4171 CCATGG RcaI 3234 TCATGA T^CATGA 4008 TCATGA SacII 39 CCGCGG CCGC^GG 207 CCGCGG ScaI 1850 AGTACT AGT^ACT 3388 AGTACT SmaI 2276 CCCGGG CCC^GGG 2890 CCCGGG XmaCI 2276 CCCGGG C^CCGGG 2890 CCCGGG XmaI 2276 CCCGGG C^CCGGG 2890 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 132 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI ApaLI AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BclI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII