################################## # NO CUT ENZYMES ( 51 enzymes) # ################################## AatII AclI AflII AgeI AscI AseI AsnI BbrPI BclI BfrI BsaAI BsaBI BsiWI BspDI BspHI BssHII Bst1107I BstZ17I ClaI EcoRV EcoT22I FbaI FseI KspI MamI MfeI MluI MunI NdeI NotI NruI NsiI PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I RcaI SacII SalI SbfI ScaI SexAI SgrAI SnaBI SpeI Sse8387I SspI SwaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### Acc65I 490 GGTACC G^GTACC AflIII 2295 ACATGT A^C[GA][CT]GT Asp700I 5194 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 490 GGTACC G^GTACC AvrII 6399 CCTAGG C^CTAGG BbeI 672 GGCGCC GGCGC^C BglII 5511 AGATCT A^GATCT BlnI 6399 CCTAGG C^CTAGG BspLU11I 2295 ACATGT A^CATGT BstBI 283 TTCGAA TT^CGAA CpoI 3230 CGGACCG CG^G[AT]CCG DraI 6466 TTTAAA TTT^AAA EagI 5238 CGGCCG C^GGCCG EclXI 5238 CGGCCG C^GGCCG Eco52I 5238 CGGCCG C^GGCCG HpaI 1466 GTTAAC GTT^AAC KasI 672 GGCGCC G^GCGCC KpnI 490 GGTACC GGTAC^C NarI 672 GGCGCC GG^CGCC NspV 283 TTCGAA TT^CGAA PaeR7I 5069 CTCGAG C^TCGAG PshAI 258 GACGGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsrII 3230 CGGACCG CG^G[AT]CCG SfiI 3681 GGCCAAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 672 GGCGCC GGC^GCC SfuI 283 TTCGAA TT^CGAA SmaI 5907 CCCGGG CCC^GGG XbaI 2301 TCTAGA T^CTAGA XhoI 5069 CTCGAG C^TCGAG XmaCI 5907 CCCGGG C^CCGGG XmaI 5907 CCCGGG C^CCGGG XmnI 5194 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 1720 GTCTAC GT^[AC][GT]AC 5285 GTCTAC AcyI 672 GGCGCC G[GA]^CG[CT]C 2055 GACGCC AhdI 3214 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5156 GACATCCTGTC Aor51HI 2178 AGCGCT AGC^GCT 3978 AGCGCT AspEI 3214 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5156 GACATCCTGTC AspI 468 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4687 GACCATGTC AviII 1802 TGCGCA TGC^GCA 5752 TGCGCA BsaHI 672 GGCGCC G[GA]^CG[CT]C 2055 GACGCC BsaI 4358 GAGACC GGTCTC(1/5) 4498 GGTCTC BsmBI 210 CGTCTC CGTCTC(1/5) 2053 GAGACG BsmI 388 GAATGC GAATGC(1/-1) 3955 GAATGC Bsp1407I 4914 TGTACA T^GTACA 5478 TGTACA BsrDI 3108 CATTGC GCAATG(2/0) 766 GCAATG BsrGI 4914 TGTACA T^GTACA 5478 TGTACA BssSI 2932 CACGAG CACGAG(-5/-1) 2434 CTCGTG DraIII 2431 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG 3364 CACTATGTG DrdI 396 GACCACAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2443 GACAAGCTTGTC Eam1105I 3214 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5156 GACATCCTGTC Eco47III 2178 AGCGCT AGC^GCT 3978 AGCGCT FspI 1802 TGCGCA TGC^GCA 5752 TGCGCA Hin1I 672 GGCGCC G[GA]^CG[CT]C 2055 GACGCC HincII 1466 GTTAAC GT[CT]^[GA]AC 3649 GTTGAC HindII 1466 GTTAAC GT[CT]^[GA]AC 3649 GTTGAC NaeI 302 GCCGGC GCC^GGC 5240 GCCGGC NgoMIV 302 GCCGGC G^CCGGC 5240 GCCGGC NheI 3171 GCTAGC G^CTAGC 3190 GCTAGC PflFI 468 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4687 GACCATGTC PvuI 2826 CGATCG CGAT^CG 5692 CGATCG SspBI 4914 TGTACA T^GTACA 5478 TGTACA Tth111I 468 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4687 GACCATGTC ######################################### # 3 OR MORE CUT ENZYMES ( 145 enzymes) # ######################################### AccII AccIII AciI AcsI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BcgI BciVI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII