##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII AclI AflII AgeI AscI
AseI AsnI BbrPI BclI BfrI
BsaAI BsaBI BsiWI BspDI BspHI
BssHII Bst1107I BstZ17I ClaI EcoRV
EcoT22I FbaI FseI KspI MamI
MfeI MluI MunI NdeI NotI
NruI NsiI PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I RcaI
SacII SalI SbfI ScaI SexAI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 490 GGTACC G^GTACC
AflIII 2295 ACATGT A^C[GA][CT]GT
Asp700I 5194 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 490 GGTACC G^GTACC
AvrII 6399 CCTAGG C^CTAGG
BbeI 672 GGCGCC GGCGC^C
BglII 5511 AGATCT A^GATCT
BlnI 6399 CCTAGG C^CTAGG
BspLU11I 2295 ACATGT A^CATGT
BstBI 283 TTCGAA TT^CGAA
CpoI 3230 CGGACCG CG^G[AT]CCG
DraI 6466 TTTAAA TTT^AAA
EagI 5238 CGGCCG C^GGCCG
EclXI 5238 CGGCCG C^GGCCG
Eco52I 5238 CGGCCG C^GGCCG
HpaI 1466 GTTAAC GTT^AAC
KasI 672 GGCGCC G^GCGCC
KpnI 490 GGTACC GGTAC^C
NarI 672 GGCGCC GG^CGCC
NspV 283 TTCGAA TT^CGAA
PaeR7I 5069 CTCGAG C^TCGAG
PshAI 258 GACGGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 3230 CGGACCG CG^G[AT]CCG
SfiI 3681 GGCCAAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 672 GGCGCC GGC^GCC
SfuI 283 TTCGAA TT^CGAA
SmaI 5907 CCCGGG CCC^GGG
XbaI 2301 TCTAGA T^CTAGA
XhoI 5069 CTCGAG C^TCGAG
XmaCI 5907 CCCGGG C^CCGGG
XmaI 5907 CCCGGG C^CCGGG
XmnI 5194 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1720 GTCTAC GT^[AC][GT]AC
5285 GTCTAC
AcyI 672 GGCGCC G[GA]^CG[CT]C
2055 GACGCC
AhdI 3214 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5156 GACATCCTGTC
Aor51HI 2178 AGCGCT AGC^GCT
3978 AGCGCT
AspEI 3214 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5156 GACATCCTGTC
AspI 468 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4687 GACCATGTC
AviII 1802 TGCGCA TGC^GCA
5752 TGCGCA
BsaHI 672 GGCGCC G[GA]^CG[CT]C
2055 GACGCC
BsaI 4358 GAGACC GGTCTC(1/5)
4498 GGTCTC
BsmBI 210 CGTCTC CGTCTC(1/5)
2053 GAGACG
BsmI 388 GAATGC GAATGC(1/-1)
3955 GAATGC
Bsp1407I 4914 TGTACA T^GTACA
5478 TGTACA
BsrDI 3108 CATTGC GCAATG(2/0)
766 GCAATG
BsrGI 4914 TGTACA T^GTACA
5478 TGTACA
BssSI 2932 CACGAG CACGAG(-5/-1)
2434 CTCGTG
DraIII 2431 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
3364 CACTATGTG
DrdI 396 GACCACAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2443 GACAAGCTTGTC
Eam1105I 3214 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5156 GACATCCTGTC
Eco47III 2178 AGCGCT AGC^GCT
3978 AGCGCT
FspI 1802 TGCGCA TGC^GCA
5752 TGCGCA
Hin1I 672 GGCGCC G[GA]^CG[CT]C
2055 GACGCC
HincII 1466 GTTAAC GT[CT]^[GA]AC
3649 GTTGAC
HindII 1466 GTTAAC GT[CT]^[GA]AC
3649 GTTGAC
NaeI 302 GCCGGC GCC^GGC
5240 GCCGGC
NgoMIV 302 GCCGGC G^CCGGC
5240 GCCGGC
NheI 3171 GCTAGC G^CTAGC
3190 GCTAGC
PflFI 468 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4687 GACCATGTC
PvuI 2826 CGATCG CGAT^CG
5692 CGATCG
SspBI 4914 TGTACA T^GTACA
5478 TGTACA
Tth111I 468 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4687 GACCATGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccII AccIII AciI AcsI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbsI BbvCI BbvI BcgI BciVI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII