################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AhdI AscI AseI AsnI Asp718I AspEI AvrII BbrPI BbvCI BfrI BlnI BsaBI BseAI BsiWI BspDI BspEI BspLU11I Bst1107I BstBI BstZ17I ClaI CpoI DraIII DrdI Eam1105I EcoRI EcoRV FseI HpaI KpnI KspI MamI MfeI MroI MunI NcoI NdeI NheI NotI NruI NspV PacI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfuI SnaBI SpeI SphI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AgeI 3604 ACCGGT A^CCGGT Alw44I 3313 GTGCAC G^TGCAC ApaLI 3313 GTGCAC G^TGCAC AviII 2577 TGCGCA TGC^GCA BamHI 2742 GGATCC G^GATCC BbeI 1080 GGCGCC GGCGC^C BclI 782 TGATCA T^GATCA BsaAI 2783 TACGTG [CT]AC^GT[GA] BsaWI 3604 ACCGGT [AT]^CCGG[AT] Bsp1407I 3761 TGTACA T^GTACA BspHI 4538 TCATGA T^CATGA BsrGI 3761 TGTACA T^GTACA DraI 6522 TTTAAA TTT^AAA DsaI 4842 CCGTGG C^C[GA][CT]GG EcoT22I 1748 ATGCAT ATGCA^T FbaI 782 TGATCA T^GATCA FspI 2577 TGCGCA TGC^GCA KasI 1080 GGCGCC G^GCGCC NarI 1080 GGCGCC GG^CGCC NsiI 1748 ATGCAT ATGCA^T NspI 1611 GCATGT [GA]CATG^[CT] PflMI 5439 CCAAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 3604 ACCGGT A^CCGGT RcaI 4538 TCATGA T^CATGA SapI 2525 GCTCTTC GCTCTTC(1/4) SfoI 1080 GGCGCC GGC^GCC SgrAI 4042 CGCCGGCG C[GA]^CCGG[CT]G SspBI 3761 TGTACA T^GTACA SspI 6554 AATATT AAT^ATT Van91I 5439 CCAAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 732 TCTAGA T^CTAGA XcmI 1717 CCAGTCACTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 360 GTCTAC GT^[AC][GT]AC 4661 GTCTAC AcyI 1080 GGCGCC G[GA]^CG[CT]C 3825 GGCGTC Aor51HI 1889 AGCGCT AGC^GCT 3671 AGCGCT ApaI 839 GGGCCC GGGCC^C 4241 GGGCCC BalI 3599 TGGCCA TGG^CCA 5225 TGGCCA BglII 1375 AGATCT A^GATCT 3869 AGATCT BsaHI 1080 GGCGCC G[GA]^CG[CT]C 3825 GGCGTC BsiEI 2423 CGGCCG CG[GA][CT]^CG 3069 CGGCCG BsmBI 2890 GAGACG CGTCTC(1/5) 4689 GAGACG BsmI 606 GAATGC GAATGC(1/-1) 395 GCATTC BssHII 74 GCGCGC G^CGCGC 76 GCGCGC BssSI 1955 CACGAG CACGAG(-5/-1) 4157 CTCGTG BstEII 1662 GGTGACC G^GT[ACGT]ACC 5829 GGTCACC BstPI 1662 GGTGACC G^GT[ACGT]ACC 5829 GGTCACC EagI 2423 CGGCCG C^GGCCG 3069 CGGCCG EclXI 2423 CGGCCG C^GGCCG 3069 CGGCCG Eco47III 1889 AGCGCT AGC^GCT 3671 AGCGCT Eco52I 2423 CGGCCG C^GGCCG 3069 CGGCCG EcoO65I 1662 GGTGACC G^GT[ACGT]ACC 5829 GGTCACC Hin1I 1080 GGCGCC G[GA]^CG[CT]C 3825 GGCGTC HindIII 3692 AAGCTT A^AGCTT 4587 AAGCTT MluI 2636 ACGCGT A^CGCGT 3649 ACGCGT MluNI 3599 TGGCCA TGG^CCA 5225 TGGCCA MscI 3599 TGGCCA TGG^CCA 5225 TGGCCA MslI 3036 CACAGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 4426 CATTCCAGTG NaeI 4043 GCCGGC GCC^GGC 5423 GCCGGC NgoMIV 4043 GCCGGC G^CCGGC 5423 GCCGGC PaeR7I 1915 CTCGAG C^TCGAG 3256 CTCGAG SexAI 1967 ACCTGGT A^CC[AT]GGT 3429 ACCTGGT XhoI 1915 CTCGAG C^TCGAG 3256 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI Asp700I AspHI AspI AvaI AvaII BanI BanII BbsI BbvI BcgI BciVI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflFI PleI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII XmaCI XmaI XmnI