##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI AscI AseI AsnI Asp718I
AspEI AvrII BbrPI BbvCI BfrI
BlnI BsaBI BseAI BsiWI BspDI
BspEI BspLU11I Bst1107I BstBI BstZ17I
ClaI CpoI DraIII DrdI Eam1105I
EcoRI EcoRV FseI HpaI KpnI
KspI MamI MfeI MroI MunI
NcoI NdeI NheI NotI NruI
NspV PacI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfuI SnaBI SpeI SphI Sse8387I
SwaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AgeI 3604 ACCGGT A^CCGGT
Alw44I 3313 GTGCAC G^TGCAC
ApaLI 3313 GTGCAC G^TGCAC
AviII 2577 TGCGCA TGC^GCA
BamHI 2742 GGATCC G^GATCC
BbeI 1080 GGCGCC GGCGC^C
BclI 782 TGATCA T^GATCA
BsaAI 2783 TACGTG [CT]AC^GT[GA]
BsaWI 3604 ACCGGT [AT]^CCGG[AT]
Bsp1407I 3761 TGTACA T^GTACA
BspHI 4538 TCATGA T^CATGA
BsrGI 3761 TGTACA T^GTACA
DraI 6522 TTTAAA TTT^AAA
DsaI 4842 CCGTGG C^C[GA][CT]GG
EcoT22I 1748 ATGCAT ATGCA^T
FbaI 782 TGATCA T^GATCA
FspI 2577 TGCGCA TGC^GCA
KasI 1080 GGCGCC G^GCGCC
NarI 1080 GGCGCC GG^CGCC
NsiI 1748 ATGCAT ATGCA^T
NspI 1611 GCATGT [GA]CATG^[CT]
PflMI 5439 CCAAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3604 ACCGGT A^CCGGT
RcaI 4538 TCATGA T^CATGA
SapI 2525 GCTCTTC GCTCTTC(1/4)
SfoI 1080 GGCGCC GGC^GCC
SgrAI 4042 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 3761 TGTACA T^GTACA
SspI 6554 AATATT AAT^ATT
Van91I 5439 CCAAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 732 TCTAGA T^CTAGA
XcmI 1717 CCAGTCACTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 360 GTCTAC GT^[AC][GT]AC
4661 GTCTAC
AcyI 1080 GGCGCC G[GA]^CG[CT]C
3825 GGCGTC
Aor51HI 1889 AGCGCT AGC^GCT
3671 AGCGCT
ApaI 839 GGGCCC GGGCC^C
4241 GGGCCC
BalI 3599 TGGCCA TGG^CCA
5225 TGGCCA
BglII 1375 AGATCT A^GATCT
3869 AGATCT
BsaHI 1080 GGCGCC G[GA]^CG[CT]C
3825 GGCGTC
BsiEI 2423 CGGCCG CG[GA][CT]^CG
3069 CGGCCG
BsmBI 2890 GAGACG CGTCTC(1/5)
4689 GAGACG
BsmI 606 GAATGC GAATGC(1/-1)
395 GCATTC
BssHII 74 GCGCGC G^CGCGC
76 GCGCGC
BssSI 1955 CACGAG CACGAG(-5/-1)
4157 CTCGTG
BstEII 1662 GGTGACC G^GT[ACGT]ACC
5829 GGTCACC
BstPI 1662 GGTGACC G^GT[ACGT]ACC
5829 GGTCACC
EagI 2423 CGGCCG C^GGCCG
3069 CGGCCG
EclXI 2423 CGGCCG C^GGCCG
3069 CGGCCG
Eco47III 1889 AGCGCT AGC^GCT
3671 AGCGCT
Eco52I 2423 CGGCCG C^GGCCG
3069 CGGCCG
EcoO65I 1662 GGTGACC G^GT[ACGT]ACC
5829 GGTCACC
Hin1I 1080 GGCGCC G[GA]^CG[CT]C
3825 GGCGTC
HindIII 3692 AAGCTT A^AGCTT
4587 AAGCTT
MluI 2636 ACGCGT A^CGCGT
3649 ACGCGT
MluNI 3599 TGGCCA TGG^CCA
5225 TGGCCA
MscI 3599 TGGCCA TGG^CCA
5225 TGGCCA
MslI 3036 CACAGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4426 CATTCCAGTG
NaeI 4043 GCCGGC GCC^GGC
5423 GCCGGC
NgoMIV 4043 GCCGGC G^CCGGC
5423 GCCGGC
PaeR7I 1915 CTCGAG C^TCGAG
3256 CTCGAG
SexAI 1967 ACCTGGT A^CC[AT]GGT
3429 ACCTGGT
XhoI 1915 CTCGAG C^TCGAG
3256 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AspI AvaI AvaII
BanI BanII BbsI BbvI BcgI
BciVI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflFI PleI PpuMI PshAI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XhoII XmaCI XmaI XmnI