################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AccIII AclI AflII AgeI Aor51HI ApaI AscI AseI AsnI BbeI BbrPI BfrI BsaAI BsaBI BseAI BsiEI BsiWI BspDI BspEI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI EagI EclXI Eco47III Eco52I EcoO65I FseI HpaI KasI KspI MamI MfeI MluI MroI MunI NaeI NarI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspI SwaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 4315 GACGTC GACGT^C AccI 490 GTCTAC GT^[AC][GT]AC AcyI 4315 GACGTC G[GA]^CG[CT]C AflIII 4470 ACATGT A^C[GA][CT]GT AhdI 3923 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1503 GTGCAC G^TGCAC ApaLI 1503 GTGCAC G^TGCAC AspEI 3923 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3313 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 3751 CCTAGG C^CTAGG BcgI 2965 CGAGAAAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2980 TGATCA T^GATCA BlnI 3751 CCTAGG C^CTAGG BsaHI 4315 GACGTC G[GA]^CG[CT]C BsaWI 5235 ACCGGA [AT]^CCGG[AT] BspHI 4209 TCATGA T^CATGA BspLU11I 4470 ACATGT A^CATGT BsrFI 856 ACCGGC [GA]^CCGG[CT] BssHII 5337 GCGCGC G^CGCGC BstXI 1353 CCAGTAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 856 ACCGGC [GA]^CCGG[CT] DraIII 171 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 3923 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 3610 GAATTC G^AATTC FbaI 2980 TGATCA T^GATCA HaeII 659 GGCGCT [GA]GCGC^[CT] Hin1I 4315 GACGTC G[GA]^CG[CT]C NheI 3067 GCTAGC G^CTAGC PflFI 3313 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 5033 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 4209 TCATGA T^CATGA SapI 4025 GAAGAGC GCTCTTC(1/4) ScaI 3082 AGTACT AGT^ACT SpeI 3079 ACTAGT A^CTAGT Tth111I 3313 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 5071 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Asp700I 4427 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4481 GAAGTATTTC AviII 3569 TGCGCA TGC^GCA 5714 TGCGCA BamHI 2928 GGATCC G^GATCC 3451 GGATCC BciVI 2227 GGATAC GTATCC(6/5) 3887 GTATCC BcnI 2184 CCGGG CC^[GC]GG 3660 CCCGG BglII 2278 AGATCT A^GATCT 2574 AGATCT BlpI 1412 GCTGAGC GC^T[ACGT]AGC 3170 GCTCAGC Bpu1102I 1412 GCTGAGC GC^T[ACGT]AGC 3170 GCTCAGC BsmBI 4317 CGTCTC CGTCTC(1/5) 2555 GAGACG BsmI 1495 GAATGC GAATGC(1/-1) 4591 GAATGC Bsp1407I 5002 TGTACA T^GTACA 5171 TGTACA BsrBI 1658 GAGCGG CCGCTC(-3/-3) 3592 GAGCGG BsrGI 5002 TGTACA T^GTACA 5171 TGTACA BssSI 1542 CTCGTG CACGAG(-5/-1) 5343 CTCGTG BstAPI 932 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2236 GCATGCATTGC CelII 1412 GCTGAGC GC^T[ACGT]AGC 3170 GCTCAGC EcoNI 4417 CCTTCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5218 CCTGAGACAGG EcoRV 4988 GATATC GAT^ATC 5059 GATATC FspI 3569 TGCGCA TGC^GCA 5714 TGCGCA HgaI 4762 GACGC GACGC(5/10) 4868 GCGTC HincII 2491 GTTGAC GT[CT]^[GA]AC 4450 GTTGAC HindII 2491 GTTGAC GT[CT]^[GA]AC 4450 GTTGAC NciI 2184 CCGGG CC^[GC]GG 3660 CCCGG NdeI 244 CATATG CA^TATG 4705 CATATG SspBI 5002 TGTACA T^GTACA 5171 TGTACA StuI 2749 AGGCCT AGG^CCT 3438 AGGCCT XmnI 4427 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4481 GAAGTATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### Acc65I AccII AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI Asp718I AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DrdI DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NcoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII