##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccIII AclI AflII AgeI Aor51HI
ApaI AscI AseI AsnI BbeI
BbrPI BfrI BsaAI BsaBI BseAI
BsiEI BsiWI BspDI BspEI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoO65I FseI HpaI KasI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NgoMIV NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspI SwaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 4315 GACGTC GACGT^C
AccI 490 GTCTAC GT^[AC][GT]AC
AcyI 4315 GACGTC G[GA]^CG[CT]C
AflIII 4470 ACATGT A^C[GA][CT]GT
AhdI 3923 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1503 GTGCAC G^TGCAC
ApaLI 1503 GTGCAC G^TGCAC
AspEI 3923 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3313 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3751 CCTAGG C^CTAGG
BcgI 2965 CGAGAAAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2980 TGATCA T^GATCA
BlnI 3751 CCTAGG C^CTAGG
BsaHI 4315 GACGTC G[GA]^CG[CT]C
BsaWI 5235 ACCGGA [AT]^CCGG[AT]
BspHI 4209 TCATGA T^CATGA
BspLU11I 4470 ACATGT A^CATGT
BsrFI 856 ACCGGC [GA]^CCGG[CT]
BssHII 5337 GCGCGC G^CGCGC
BstXI 1353 CCAGTAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 856 ACCGGC [GA]^CCGG[CT]
DraIII 171 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3923 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3610 GAATTC G^AATTC
FbaI 2980 TGATCA T^GATCA
HaeII 659 GGCGCT [GA]GCGC^[CT]
Hin1I 4315 GACGTC G[GA]^CG[CT]C
NheI 3067 GCTAGC G^CTAGC
PflFI 3313 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 5033 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4209 TCATGA T^CATGA
SapI 4025 GAAGAGC GCTCTTC(1/4)
ScaI 3082 AGTACT AGT^ACT
SpeI 3079 ACTAGT A^CTAGT
Tth111I 3313 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5071 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Asp700I 4427 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4481 GAAGTATTTC
AviII 3569 TGCGCA TGC^GCA
5714 TGCGCA
BamHI 2928 GGATCC G^GATCC
3451 GGATCC
BciVI 2227 GGATAC GTATCC(6/5)
3887 GTATCC
BcnI 2184 CCGGG CC^[GC]GG
3660 CCCGG
BglII 2278 AGATCT A^GATCT
2574 AGATCT
BlpI 1412 GCTGAGC GC^T[ACGT]AGC
3170 GCTCAGC
Bpu1102I 1412 GCTGAGC GC^T[ACGT]AGC
3170 GCTCAGC
BsmBI 4317 CGTCTC CGTCTC(1/5)
2555 GAGACG
BsmI 1495 GAATGC GAATGC(1/-1)
4591 GAATGC
Bsp1407I 5002 TGTACA T^GTACA
5171 TGTACA
BsrBI 1658 GAGCGG CCGCTC(-3/-3)
3592 GAGCGG
BsrGI 5002 TGTACA T^GTACA
5171 TGTACA
BssSI 1542 CTCGTG CACGAG(-5/-1)
5343 CTCGTG
BstAPI 932 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2236 GCATGCATTGC
CelII 1412 GCTGAGC GC^T[ACGT]AGC
3170 GCTCAGC
EcoNI 4417 CCTTCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5218 CCTGAGACAGG
EcoRV 4988 GATATC GAT^ATC
5059 GATATC
FspI 3569 TGCGCA TGC^GCA
5714 TGCGCA
HgaI 4762 GACGC GACGC(5/10)
4868 GCGTC
HincII 2491 GTTGAC GT[CT]^[GA]AC
4450 GTTGAC
HindII 2491 GTTGAC GT[CT]^[GA]AC
4450 GTTGAC
NciI 2184 CCGGG CC^[GC]GG
3660 CCCGG
NdeI 244 CATATG CA^TATG
4705 CATATG
SspBI 5002 TGTACA T^GTACA
5171 TGTACA
StuI 2749 AGGCCT AGG^CCT
3438 AGGCCT
XmnI 4427 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4481 GAAGTATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
Acc65I AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApoI
Asp718I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BfaI BglI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DrdI DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NcoI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII