##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII Acc65I AclI AscI Asp700I
Asp718I AspI AviII AvrII BbrPI
BlnI BsaBI BsiEI BsiWI BsmI
BssHII Bst1107I BstZ17I CpoI EagI
EclXI Eco52I EcoRI FseI FspI
HincII HindII HpaI KpnI MamI
MfeI MluI MunI NcoI NotI
NruI PacI PflFI PmaCI PmlI
Psp1406I PvuI RsrII SacI SalI
SfiI SgrAI SnaBI SwaI Tth111I
XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccIII 683 TCCGGA T^CCGGA
AcyI 779 GGCGCC G[GA]^CG[CT]C
AflIII 1292 ACATGT A^C[GA][CT]GT
AhdI 4557 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3562 GTGCAC G^TGCAC
ApaI 4210 GGGCCC GGGCC^C
ApaLI 3562 GTGCAC G^TGCAC
AspEI 4557 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2843 TGGCCA TGG^CCA
BamHI 5228 GGATCC G^GATCC
BbeI 779 GGCGCC GGCGC^C
BcgI 1122 CGATCAGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 332 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 558 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 558 GCTGAGC GC^T[ACGT]AGC
BsaAI 769 TACGTG [CT]AC^GT[GA]
BsaHI 779 GGCGCC G[GA]^CG[CT]C
BseAI 683 TCCGGA T^CCGGA
BsmBI 128 GAGACG CGTCTC(1/5)
BspDI 2087 ATCGAT AT^CGAT
BspEI 683 TCCGGA T^CCGGA
BspHI 2870 TCATGA T^CATGA
BspLU11I 1292 ACATGT A^CATGT
BstBI 1460 TTCGAA TT^CGAA
CelII 558 GCTGAGC GC^T[ACGT]AGC
ClaI 2087 ATCGAT AT^CGAT
DrdI 5048 GACAGCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4557 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Hin1I 779 GGCGCC G[GA]^CG[CT]C
HindIII 5650 AAGCTT A^AGCTT
KasI 779 GGCGCC G^GCGCC
KspI 49 CCGCGG CCGC^GG
MluNI 2843 TGGCCA TGG^CCA
MroI 683 TCCGGA T^CCGGA
MscI 2843 TGGCCA TGG^CCA
NaeI 776 GCCGGC GCC^GGC
NarI 779 GGCGCC GG^CGCC
NgoMIV 776 GCCGGC G^CCGGC
NspV 1460 TTCGAA TT^CGAA
PaeR7I 1244 CTCGAG C^TCGAG
PflMI 1558 CCACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 3833 GTTTAAAC GTTT^AAAC
RcaI 2870 TCATGA T^CATGA
SacII 49 CCGCGG CCGC^GG
SbfI 3686 CCTGCAGG CCTGCA^GG
SexAI 2864 ACCTGGT A^CC[AT]GGT
SfoI 779 GGCGCC GGC^GCC
SfuI 1460 TTCGAA TT^CGAA
SmaI 449 CCCGGG CCC^GGG
Sse8387I 3686 CCTGCAGG CCTGCA^GG
StuI 1237 AGGCCT AGG^CCT
Van91I 1558 CCACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1287 TCTAGA T^CTAGA
XhoI 1244 CTCGAG C^TCGAG
XmaCI 449 CCCGGG C^CCGGG
XmaI 449 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AgeI 932 ACCGGT A^CCGGT
1791 ACCGGT
Aor51HI 167 AGCGCT AGC^GCT
188 AGCGCT
AseI 10 ATTAAT AT^TAAT
3728 ATTAAT
AsnI 10 ATTAAT AT^TAAT
3728 ATTAAT
BbvCI 2645 CCTCAGC CCTCAGC(-5/-2)
4050 GCTGAGG
BglII 4867 AGATCT A^GATCT
5928 AGATCT
BsaI 355 GAGACC GGTCTC(1/5)
2174 GAGACC
BstEII 2446 GGTGACC G^GT[ACGT]ACC
5674 GGTAACC
BstPI 2446 GGTGACC G^GT[ACGT]ACC
5674 GGTAACC
Eco47III 167 AGCGCT AGC^GCT
188 AGCGCT
EcoO65I 2446 GGTGACC G^GT[ACGT]ACC
5674 GGTAACC
EcoRV 1107 GATATC GAT^ATC
1162 GATATC
EcoT22I 2431 ATGCAT ATGCA^T
5132 ATGCAT
MaeII 471 ACGT A^CGT
770 ACGT
NdeI 1543 CATATG CA^TATG
5135 CATATG
NheI 2461 GCTAGC G^CTAGC
4704 GCTAGC
NsiI 2431 ATGCAT ATGCA^T
5132 ATGCAT
PinAI 932 ACCGGT A^CCGGT
1791 ACCGGT
PshAI 840 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2035 GACCGAAGTC
PshBI 10 ATTAAT AT^TAAT
3728 ATTAAT
PstI 3687 CTGCAG CTGCA^G
5869 CTGCAG
SapI 2154 GAAGAGC GCTCTTC(1/4)
4127 GCTCTTC
ScaI 4191 AGTACT AGT^ACT
5327 AGTACT
SphI 714 GCATGC GCATG^C
2393 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BanI
BanII BbsI BbvI BciVI BclI
BcnI BfaI BfrI BmyI BpmI
BpuAI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SpeI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII