################################## # NO CUT ENZYMES ( 51 enzymes) # ################################## AatII Acc65I AclI AscI Asp700I Asp718I AspI AviII AvrII BbrPI BlnI BsaBI BsiEI BsiWI BsmI BssHII Bst1107I BstZ17I CpoI EagI EclXI Eco52I EcoRI FseI FspI HincII HindII HpaI KpnI MamI MfeI MluI MunI NcoI NotI NruI PacI PflFI PmaCI PmlI Psp1406I PvuI RsrII SacI SalI SfiI SgrAI SnaBI SwaI Tth111I XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccIII 683 TCCGGA T^CCGGA AcyI 779 GGCGCC G[GA]^CG[CT]C AflIII 1292 ACATGT A^C[GA][CT]GT AhdI 4557 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3562 GTGCAC G^TGCAC ApaI 4210 GGGCCC GGGCC^C ApaLI 3562 GTGCAC G^TGCAC AspEI 4557 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 2843 TGGCCA TGG^CCA BamHI 5228 GGATCC G^GATCC BbeI 779 GGCGCC GGCGC^C BcgI 1122 CGATCAGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 332 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 558 GCTGAGC GC^T[ACGT]AGC Bpu1102I 558 GCTGAGC GC^T[ACGT]AGC BsaAI 769 TACGTG [CT]AC^GT[GA] BsaHI 779 GGCGCC G[GA]^CG[CT]C BseAI 683 TCCGGA T^CCGGA BsmBI 128 GAGACG CGTCTC(1/5) BspDI 2087 ATCGAT AT^CGAT BspEI 683 TCCGGA T^CCGGA BspHI 2870 TCATGA T^CATGA BspLU11I 1292 ACATGT A^CATGT BstBI 1460 TTCGAA TT^CGAA CelII 558 GCTGAGC GC^T[ACGT]AGC ClaI 2087 ATCGAT AT^CGAT DrdI 5048 GACAGCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 4557 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Hin1I 779 GGCGCC G[GA]^CG[CT]C HindIII 5650 AAGCTT A^AGCTT KasI 779 GGCGCC G^GCGCC KspI 49 CCGCGG CCGC^GG MluNI 2843 TGGCCA TGG^CCA MroI 683 TCCGGA T^CCGGA MscI 2843 TGGCCA TGG^CCA NaeI 776 GCCGGC GCC^GGC NarI 779 GGCGCC GG^CGCC NgoMIV 776 GCCGGC G^CCGGC NspV 1460 TTCGAA TT^CGAA PaeR7I 1244 CTCGAG C^TCGAG PflMI 1558 CCACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmeI 3833 GTTTAAAC GTTT^AAAC RcaI 2870 TCATGA T^CATGA SacII 49 CCGCGG CCGC^GG SbfI 3686 CCTGCAGG CCTGCA^GG SexAI 2864 ACCTGGT A^CC[AT]GGT SfoI 779 GGCGCC GGC^GCC SfuI 1460 TTCGAA TT^CGAA SmaI 449 CCCGGG CCC^GGG Sse8387I 3686 CCTGCAGG CCTGCA^GG StuI 1237 AGGCCT AGG^CCT Van91I 1558 CCACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1287 TCTAGA T^CTAGA XhoI 1244 CTCGAG C^TCGAG XmaCI 449 CCCGGG C^CCGGG XmaI 449 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AgeI 932 ACCGGT A^CCGGT 1791 ACCGGT Aor51HI 167 AGCGCT AGC^GCT 188 AGCGCT AseI 10 ATTAAT AT^TAAT 3728 ATTAAT AsnI 10 ATTAAT AT^TAAT 3728 ATTAAT BbvCI 2645 CCTCAGC CCTCAGC(-5/-2) 4050 GCTGAGG BglII 4867 AGATCT A^GATCT 5928 AGATCT BsaI 355 GAGACC GGTCTC(1/5) 2174 GAGACC BstEII 2446 GGTGACC G^GT[ACGT]ACC 5674 GGTAACC BstPI 2446 GGTGACC G^GT[ACGT]ACC 5674 GGTAACC Eco47III 167 AGCGCT AGC^GCT 188 AGCGCT EcoO65I 2446 GGTGACC G^GT[ACGT]ACC 5674 GGTAACC EcoRV 1107 GATATC GAT^ATC 1162 GATATC EcoT22I 2431 ATGCAT ATGCA^T 5132 ATGCAT MaeII 471 ACGT A^CGT 770 ACGT NdeI 1543 CATATG CA^TATG 5135 CATATG NheI 2461 GCTAGC G^CTAGC 4704 GCTAGC NsiI 2431 ATGCAT ATGCA^T 5132 ATGCAT PinAI 932 ACCGGT A^CCGGT 1791 ACCGGT PshAI 840 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2035 GACCGAAGTC PshBI 10 ATTAAT AT^TAAT 3728 ATTAAT PstI 3687 CTGCAG CTGCA^G 5869 CTGCAG SapI 2154 GAAGAGC GCTCTTC(1/4) 4127 GCTCTTC ScaI 4191 AGTACT AGT^ACT 5327 AGTACT SphI 714 GCATGC GCATG^C 2393 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 127 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflII AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BciVI BclI BcnI BfaI BfrI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SpeI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII