##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AccIII AclI Alw44I ApaLI
AscI AseI AsnI Asp718I AvrII
BglII BlnI BsaBI BseAI BsiEI
BsiWI BspDI BspEI BsrDI Bst1107I
BstBI BstZ17I ClaI EagI EclXI
Eco52I FseI HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PmeI
PshBI Psp1406I PvuI SacII SalI
SbfI ScaI SfuI SgrAI SnaBI
Sse8387I SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AflII 766 CTTAAG C^TTAAG
AgeI 3079 ACCGGT A^CCGGT
Aor51HI 5591 AGCGCT AGC^GCT
ApaI 5015 GGGCCC GGGCC^C
AviII 2015 TGCGCA TGC^GCA
BbeI 5562 GGCGCC GGCGC^C
BbrPI 2809 CACGTG CAC^GTG
BciVI 3329 GTATCC GTATCC(6/5)
BclI 4160 TGATCA T^GATCA
BfrI 766 CTTAAG C^TTAAG
BglI 3748 GCCATCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2809 CACGTG [CT]AC^GT[GA]
Bsp1407I 5222 TGTACA T^GTACA
BsrGI 5222 TGTACA T^GTACA
BssHII 5418 GCGCGC G^CGCGC
BssSI 29 CTCGTG CACGAG(-5/-1)
BstEII 2463 GGTGACC G^GT[ACGT]ACC
BstPI 2463 GGTGACC G^GT[ACGT]ACC
CpoI 3654 CGGACCG CG^G[AT]CCG
Eco47III 5591 AGCGCT AGC^GCT
EcoO65I 2463 GGTGACC G^GT[ACGT]ACC
EcoRI 3674 GAATTC G^AATTC
EcoRV 1454 GATATC GAT^ATC
EcoT22I 1048 ATGCAT ATGCA^T
FbaI 4160 TGATCA T^GATCA
FspI 2015 TGCGCA TGC^GCA
KasI 5562 GGCGCC G^GCGCC
NarI 5562 GGCGCC GG^CGCC
NsiI 1048 ATGCAT ATGCA^T
PinAI 3079 ACCGGT A^CCGGT
PmaCI 2809 CACGTG CAC^GTG
PmlI 2809 CACGTG CAC^GTG
RsrII 3654 CGGACCG CG^G[AT]CCG
SexAI 2729 ACCTGGT A^CC[AT]GGT
SfiI 3747 GGCCATCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 5562 GGCGCC GGC^GCC
SpeI 4530 ACTAGT A^CTAGT
SspBI 5222 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AatII 3775 GACGTC GACGT^C
5470 GACGTC
AhdI 3878 GACTGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5470 GACGTCTGGTC
Asp700I 1326 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5238 GAAACGGTTC
AspEI 3878 GACTGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5470 GACGTCTGGTC
AspI 2728 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3253 GACAAAGTC
BamHI 1517 GGATCC G^GATCC
1974 GGATCC
BcgI 2127 CGACCACGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2583 GCAGGGCATTCG
BsaI 2247 GGTCTC GGTCTC(1/5)
2368 GGTCTC
BsrFI 3079 ACCGGT [GA]^CCGG[CT]
4910 ACCGGC
Cfr10I 3079 ACCGGT [GA]^CCGG[CT]
4910 ACCGGC
DraI 5912 TTTAAA TTT^AAA
6031 TTTAAA
DrdI 3213 GACTGTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3769 GACCCAGACGTC
Eam1105I 3878 GACTGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5470 GACGTCTGGTC
HindIII 135 AAGCTT A^AGCTT
616 AAGCTT
NcoI 714 CCATGG C^CATGG
3842 CCATGG
PflFI 2728 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3253 GACAAAGTC
PvuII 4774 CAGCTG CAG^CTG
5389 CAGCTG
SphI 677 GCATGC GCATG^C
5813 GCATGC
Tth111I 2728 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3253 GACAAAGTC
XmnI 1326 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5238 GAAACGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspHI BspLU11I BspMI
BsrBI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PshAI PstI RcaI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI