################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AccII AccIII AhdI Alw44I ApaI ApaLI AscI AspEI AviII BamHI BbeI BciVI BcnI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BssHII BssSI BstUI ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoRI FseI FspI KasI KspI MfeI MluI MroI MunI MvnI NaeI NarI NciI NcoI NgoMIV NotI NruI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SmaI SnaBI SpeI Sse8387I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AatII 5695 GACGTC GACGT^C Acc65I 4486 GGTACC G^GTACC AclI 2758 AACGTT AA^CGTT AcyI 5695 GACGTC G[GA]^CG[CT]C AgeI 5008 ACCGGT A^CCGGT AlwNI 1200 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 5118 AGCGCT AGC^GCT Asp700I 4147 GAACGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 4486 GGTACC G^GTACC AspI 701 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 5283 CTCGAG C^[CT]CG[GA]G AvrII 6714 CCTAGG C^CTAGG BanI 4486 GGTACC G^G[CT][GA]CC BbrPI 680 CACGTG CAC^GTG BcgI 5272 CGATATAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 4703 TGATCA T^GATCA BglI 4041 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 6714 CCTAGG C^CTAGG BsaBI 2481 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 5695 GACGTC G[GA]^CG[CT]C BsaI 6311 GAGACC GGTCTC(1/5) BseRI 135 CTCCTC GAGGAG(10/8) BsoBI 5283 CTCGAG C^[CT]CG[GA]G BsrFI 5008 ACCGGT [GA]^CCGG[CT] Bst1107I 4845 GTATAC GTA^TAC BstBI 282 TTCGAA TT^CGAA BstEII 2899 GGTAACC G^GT[ACGT]ACC BstPI 2899 GGTAACC G^GT[ACGT]ACC BstZ17I 4845 GTATAC GTA^TAC Cfr10I 5008 ACCGGT [GA]^CCGG[CT] DsaI 16 CCACGG C^C[GA][CT]GG Eco47III 5118 AGCGCT AGC^GCT EcoO65I 2899 GGTAACC G^GT[ACGT]ACC EcoT22I 4823 ATGCAT ATGCA^T FauI 3195 CCCGC CCCGC(4/6) FbaI 4703 TGATCA T^GATCA HgaI 3272 GACGC GACGC(5/10) Hin1I 5695 GACGTC G[GA]^CG[CT]C HpaI 2521 GTTAAC GTT^AAC KpnI 4486 GGTACC GGTAC^C MamI 2481 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NheI 918 GCTAGC G^CTAGC NsiI 4823 ATGCAT ATGCA^T NspV 282 TTCGAA TT^CGAA PacI 1675 TTAATTAA TTAAT^TAA PaeR7I 5283 CTCGAG C^TCGAG PflFI 701 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 5008 ACCGGT A^CCGGT PmaCI 680 CACGTG CAC^GTG PmeI 5199 GTTTAAAC GTTT^AAAC PmlI 680 CACGTG CAC^GTG PshAI 1730 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 2758 AACGTT AA^CGTT SacI 3982 GAGCTC GAGCT^C SapI 3356 GAAGAGC GCTCTTC(1/4) SfuI 282 TTCGAA TT^CGAA SgrAI 5007 CACCGGTG C[GA]^CCGG[CT]G SphI 3765 GCATGC GCATG^C SwaI 309 ATTTAAAT ATTT^AAAT Tth111I 701 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 5283 CTCGAG C^TCGAG XmnI 4147 GAACGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 4053 GTCTAC GT^[AC][GT]AC 4845 GTATAC BalI 118 TGGCCA TGG^CCA 5028 TGGCCA BbvCI 945 CCTCAGC CCTCAGC(-5/-2) 995 GCTGAGG BsaWI 3148 TCCGGT [AT]^CCGG[AT] 5008 ACCGGT BspHI 4649 TCATGA T^CATGA 5395 TCATGA BspMI 5636 ACCTGC ACCTGC(4/8) 3674 GCAGGT BstAPI 3361 GCAGTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3612 GCACTGAGTGC CfoI 4299 GCGC GCG^C 5119 GCGC DraII 934 GGGTCCT [GA]G^G[ACGT]CC[CT] 3346 AGGACCT EcoNI 6576 CCTATGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 6811 CCTTCAGAAGG EcoO109I 934 GGGTCCT [GA]G^G[ACGT]CC[CT] 3346 AGGACCT HaeII 4298 AGCGCC [GA]GCGC^[CT] 5118 AGCGCT HapII 3149 CCGG C^CGG 5009 CCGG HhaI 4299 GCGC GCG^C 5119 GCGC HinP1I 4299 GCGC G^CGC 5119 GCGC HpaII 3149 CCGG C^CGG 5009 CCGG MluNI 118 TGGCCA TGG^CCA 5028 TGGCCA MscI 118 TGGCCA TGG^CCA 5028 TGGCCA MspI 3149 CCGG C^CGG 5009 CCGG PflMI 4177 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5115 CCAAGCGCTGG PpuMI 934 GGGTCCT [GA]G^G[AT]CC[CT] 3346 AGGACCT PstI 225 CTGCAG CTGCA^G 3672 CTGCAG PvuII 993 CAGCTG CAG^CTG 2398 CAGCTG RcaI 4649 TCATGA T^CATGA 5395 TCATGA Van91I 4177 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5115 CCAAGCGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AciI AcsI AfaI AflII AflIII AluI AlwI AocI ApoI AseI AsnI AspHI AvaII BanII BbsI BbvI BfaI BfrI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspLU11I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII Cfr13I DdeI DpnII DraI DraIII EaeI EarI Eco81I EcoRII EcoRV EcoT14I Fnu4HI FokI HaeIII HincII HindII HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeI NdeII NlaIII NlaIV NspI PleI PshBI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII