##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccII AccIII AhdI Alw44I ApaI
ApaLI AscI AspEI AviII BamHI
BbeI BciVI BcnI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BssHII BssSI BstUI ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
EcoRI FseI FspI KasI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NotI NruI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SmaI SnaBI SpeI Sse8387I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AatII 5695 GACGTC GACGT^C
Acc65I 4486 GGTACC G^GTACC
AclI 2758 AACGTT AA^CGTT
AcyI 5695 GACGTC G[GA]^CG[CT]C
AgeI 5008 ACCGGT A^CCGGT
AlwNI 1200 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 5118 AGCGCT AGC^GCT
Asp700I 4147 GAACGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4486 GGTACC G^GTACC
AspI 701 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 5283 CTCGAG C^[CT]CG[GA]G
AvrII 6714 CCTAGG C^CTAGG
BanI 4486 GGTACC G^G[CT][GA]CC
BbrPI 680 CACGTG CAC^GTG
BcgI 5272 CGATATAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4703 TGATCA T^GATCA
BglI 4041 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 6714 CCTAGG C^CTAGG
BsaBI 2481 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 5695 GACGTC G[GA]^CG[CT]C
BsaI 6311 GAGACC GGTCTC(1/5)
BseRI 135 CTCCTC GAGGAG(10/8)
BsoBI 5283 CTCGAG C^[CT]CG[GA]G
BsrFI 5008 ACCGGT [GA]^CCGG[CT]
Bst1107I 4845 GTATAC GTA^TAC
BstBI 282 TTCGAA TT^CGAA
BstEII 2899 GGTAACC G^GT[ACGT]ACC
BstPI 2899 GGTAACC G^GT[ACGT]ACC
BstZ17I 4845 GTATAC GTA^TAC
Cfr10I 5008 ACCGGT [GA]^CCGG[CT]
DsaI 16 CCACGG C^C[GA][CT]GG
Eco47III 5118 AGCGCT AGC^GCT
EcoO65I 2899 GGTAACC G^GT[ACGT]ACC
EcoT22I 4823 ATGCAT ATGCA^T
FauI 3195 CCCGC CCCGC(4/6)
FbaI 4703 TGATCA T^GATCA
HgaI 3272 GACGC GACGC(5/10)
Hin1I 5695 GACGTC G[GA]^CG[CT]C
HpaI 2521 GTTAAC GTT^AAC
KpnI 4486 GGTACC GGTAC^C
MamI 2481 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 918 GCTAGC G^CTAGC
NsiI 4823 ATGCAT ATGCA^T
NspV 282 TTCGAA TT^CGAA
PacI 1675 TTAATTAA TTAAT^TAA
PaeR7I 5283 CTCGAG C^TCGAG
PflFI 701 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 5008 ACCGGT A^CCGGT
PmaCI 680 CACGTG CAC^GTG
PmeI 5199 GTTTAAAC GTTT^AAAC
PmlI 680 CACGTG CAC^GTG
PshAI 1730 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2758 AACGTT AA^CGTT
SacI 3982 GAGCTC GAGCT^C
SapI 3356 GAAGAGC GCTCTTC(1/4)
SfuI 282 TTCGAA TT^CGAA
SgrAI 5007 CACCGGTG C[GA]^CCGG[CT]G
SphI 3765 GCATGC GCATG^C
SwaI 309 ATTTAAAT ATTT^AAAT
Tth111I 701 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 5283 CTCGAG C^TCGAG
XmnI 4147 GAACGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 4053 GTCTAC GT^[AC][GT]AC
4845 GTATAC
BalI 118 TGGCCA TGG^CCA
5028 TGGCCA
BbvCI 945 CCTCAGC CCTCAGC(-5/-2)
995 GCTGAGG
BsaWI 3148 TCCGGT [AT]^CCGG[AT]
5008 ACCGGT
BspHI 4649 TCATGA T^CATGA
5395 TCATGA
BspMI 5636 ACCTGC ACCTGC(4/8)
3674 GCAGGT
BstAPI 3361 GCAGTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3612 GCACTGAGTGC
CfoI 4299 GCGC GCG^C
5119 GCGC
DraII 934 GGGTCCT [GA]G^G[ACGT]CC[CT]
3346 AGGACCT
EcoNI 6576 CCTATGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6811 CCTTCAGAAGG
EcoO109I 934 GGGTCCT [GA]G^G[ACGT]CC[CT]
3346 AGGACCT
HaeII 4298 AGCGCC [GA]GCGC^[CT]
5118 AGCGCT
HapII 3149 CCGG C^CGG
5009 CCGG
HhaI 4299 GCGC GCG^C
5119 GCGC
HinP1I 4299 GCGC G^CGC
5119 GCGC
HpaII 3149 CCGG C^CGG
5009 CCGG
MluNI 118 TGGCCA TGG^CCA
5028 TGGCCA
MscI 118 TGGCCA TGG^CCA
5028 TGGCCA
MspI 3149 CCGG C^CGG
5009 CCGG
PflMI 4177 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5115 CCAAGCGCTGG
PpuMI 934 GGGTCCT [GA]G^G[AT]CC[CT]
3346 AGGACCT
PstI 225 CTGCAG CTGCA^G
3672 CTGCAG
PvuII 993 CAGCTG CAG^CTG
2398 CAGCTG
RcaI 4649 TCATGA T^CATGA
5395 TCATGA
Van91I 4177 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5115 CCAAGCGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AocI ApoI AseI
AsnI AspHI AvaII BanII BbsI
BbvI BfaI BfrI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspLU11I BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII Cfr13I DdeI DpnII
DraI DraIII EaeI EarI Eco81I
EcoRII EcoRV EcoT14I Fnu4HI FokI
HaeIII HincII HindII HindIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeI NdeII NlaIII NlaIV NspI
PleI PshBI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspBI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII