##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AatII AgeI Aor51HI AscI AseI
AsnI Asp700I BbeI BsiWI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
CpoI Eco47III EcoO65I EcoRV FseI
HindIII KasI KspI MfeI MunI
NarI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshBI PvuI
RsrII SacII SalI SexAI SfoI
SfuI SmaI SnaBI SpeI SspI
SwaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 4087 GGTACC G^GTACC
AclI 397 AACGTT AA^CGTT
AflII 5629 CTTAAG C^TTAAG
Asp718I 4087 GGTACC G^GTACC
AviII 608 TGCGCA TGC^GCA
AvrII 1285 CCTAGG C^CTAGG
BamHI 6464 GGATCC G^GATCC
BbrPI 4428 CACGTG CAC^GTG
BciVI 5443 GTATCC GTATCC(6/5)
BclI 2927 TGATCA T^GATCA
BfrI 5629 CTTAAG C^TTAAG
BlnI 1285 CCTAGG C^CTAGG
BsaBI 2295 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 5906 GCATTC GAATGC(1/-1)
BspHI 2096 TCATGA T^CATGA
BspLU11I 4992 ACATGT A^CATGT
DraIII 5839 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1552 GACGGGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 6740 GAATTC G^AATTC
EcoT22I 1820 ATGCAT ATGCA^T
FbaI 2927 TGATCA T^GATCA
FspI 608 TGCGCA TGC^GCA
KpnI 4087 GGTACC GGTAC^C
MamI 2295 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1341 ACGCGT A^CGCGT
NsiI 1820 ATGCAT ATGCA^T
PmaCI 4428 CACGTG CAC^GTG
PmlI 4428 CACGTG CAC^GTG
PshAI 4652 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 397 AACGTT AA^CGTT
RcaI 2096 TCATGA T^CATGA
SfiI 3670 GGCCACCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2317 CACCGGCG C[GA]^CCGG[CT]G
SmlI 5629 CTTAAG C^T[CT][GA]AG
SphI 1697 GCATGC GCATG^C
XbaI 4454 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 5880 GTAGAC GT^[AC][GT]AC
6136 GTAGAC
AcsI 6300 AAATTC [GA]^AATT[CT]
6740 GAATTC
AflIII 1341 ACGCGT A^C[GA][CT]GT
4992 ACATGT
AhdI 2808 GACCCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4862 GACCTCCTGTC
Alw44I 686 GTGCAC G^TGCAC
4244 GTGCAC
AocI 2286 CCTGAGG CC^T[ACGT]AGG
5108 CCTCAGG
ApaLI 686 GTGCAC G^TGCAC
4244 GTGCAC
ApoI 6300 AAATTC [GA]^AATT[CT]
6740 GAATTC
AspEI 2808 GACCCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4862 GACCTCCTGTC
BcgI 2195 CGATTGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4537 CGAGCCATATGC
BglII 2144 AGATCT A^GATCT
2934 AGATCT
Bsp1407I 1169 TGTACA T^GTACA
6761 TGTACA
BspDI 889 ATCGAT AT^CGAT
3052 ATCGAT
BsrDI 3075 CATTGC GCAATG(2/0)
5371 GCAATG
BsrGI 1169 TGTACA T^GTACA
6761 TGTACA
BssSI 1687 CACGAG CACGAG(-5/-1)
3085 CACGAG
Bsu36I 2286 CCTGAGG CC^T[ACGT]AGG
5108 CCTCAGG
ClaI 889 ATCGAT AT^CGAT
3052 ATCGAT
DraI 5073 TTTAAA TTT^AAA
5594 TTTAAA
EagI 140 CGGCCG C^GGCCG
1521 CGGCCG
Eam1105I 2808 GACCCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4862 GACCTCCTGTC
EclXI 140 CGGCCG C^GGCCG
1521 CGGCCG
Eco52I 140 CGGCCG C^GGCCG
1521 CGGCCG
Eco81I 2286 CCTGAGG CC^T[ACGT]AGG
5108 CCTCAGG
HpaI 895 GTTAAC GTT^AAC
4708 GTTAAC
NaeI 787 GCCGGC GCC^GGC
1779 GCCGGC
NcoI 3293 CCATGG C^CATGG
4400 CCATGG
NdeI 1439 CATATG CA^TATG
4542 CATATG
NgoMIV 787 GCCGGC G^CCGGC
1779 GCCGGC
NheI 4393 GCTAGC G^CTAGC
5577 GCTAGC
SbfI 4899 CCTGCAGG CCTGCA^GG
6498 CCTGCAGG
ScaI 5262 AGTACT AGT^ACT
5980 AGTACT
Sse8387I 4899 CCTGCAGG CCTGCA^GG
6498 CCTGCAGG
SspBI 1169 TGTACA T^GTACA
6761 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspEI
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII