################################## # NO CUT ENZYMES ( 50 enzymes) # ################################## AatII AgeI Aor51HI AscI AseI AsnI Asp700I BbeI BsiWI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CpoI Eco47III EcoO65I EcoRV FseI HindIII KasI KspI MfeI MunI NarI NotI NruI NspV PacI PaeR7I PinAI PmeI PshBI PvuI RsrII SacII SalI SexAI SfoI SfuI SmaI SnaBI SpeI SspI SwaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 4087 GGTACC G^GTACC AclI 397 AACGTT AA^CGTT AflII 5629 CTTAAG C^TTAAG Asp718I 4087 GGTACC G^GTACC AviII 608 TGCGCA TGC^GCA AvrII 1285 CCTAGG C^CTAGG BamHI 6464 GGATCC G^GATCC BbrPI 4428 CACGTG CAC^GTG BciVI 5443 GTATCC GTATCC(6/5) BclI 2927 TGATCA T^GATCA BfrI 5629 CTTAAG C^TTAAG BlnI 1285 CCTAGG C^CTAGG BsaBI 2295 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 5906 GCATTC GAATGC(1/-1) BspHI 2096 TCATGA T^CATGA BspLU11I 4992 ACATGT A^CATGT DraIII 5839 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1552 GACGGGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 6740 GAATTC G^AATTC EcoT22I 1820 ATGCAT ATGCA^T FbaI 2927 TGATCA T^GATCA FspI 608 TGCGCA TGC^GCA KpnI 4087 GGTACC GGTAC^C MamI 2295 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 1341 ACGCGT A^CGCGT NsiI 1820 ATGCAT ATGCA^T PmaCI 4428 CACGTG CAC^GTG PmlI 4428 CACGTG CAC^GTG PshAI 4652 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 397 AACGTT AA^CGTT RcaI 2096 TCATGA T^CATGA SfiI 3670 GGCCACCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 2317 CACCGGCG C[GA]^CCGG[CT]G SmlI 5629 CTTAAG C^T[CT][GA]AG SphI 1697 GCATGC GCATG^C XbaI 4454 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccI 5880 GTAGAC GT^[AC][GT]AC 6136 GTAGAC AcsI 6300 AAATTC [GA]^AATT[CT] 6740 GAATTC AflIII 1341 ACGCGT A^C[GA][CT]GT 4992 ACATGT AhdI 2808 GACCCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4862 GACCTCCTGTC Alw44I 686 GTGCAC G^TGCAC 4244 GTGCAC AocI 2286 CCTGAGG CC^T[ACGT]AGG 5108 CCTCAGG ApaLI 686 GTGCAC G^TGCAC 4244 GTGCAC ApoI 6300 AAATTC [GA]^AATT[CT] 6740 GAATTC AspEI 2808 GACCCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4862 GACCTCCTGTC BcgI 2195 CGATTGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4537 CGAGCCATATGC BglII 2144 AGATCT A^GATCT 2934 AGATCT Bsp1407I 1169 TGTACA T^GTACA 6761 TGTACA BspDI 889 ATCGAT AT^CGAT 3052 ATCGAT BsrDI 3075 CATTGC GCAATG(2/0) 5371 GCAATG BsrGI 1169 TGTACA T^GTACA 6761 TGTACA BssSI 1687 CACGAG CACGAG(-5/-1) 3085 CACGAG Bsu36I 2286 CCTGAGG CC^T[ACGT]AGG 5108 CCTCAGG ClaI 889 ATCGAT AT^CGAT 3052 ATCGAT DraI 5073 TTTAAA TTT^AAA 5594 TTTAAA EagI 140 CGGCCG C^GGCCG 1521 CGGCCG Eam1105I 2808 GACCCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4862 GACCTCCTGTC EclXI 140 CGGCCG C^GGCCG 1521 CGGCCG Eco52I 140 CGGCCG C^GGCCG 1521 CGGCCG Eco81I 2286 CCTGAGG CC^T[ACGT]AGG 5108 CCTCAGG HpaI 895 GTTAAC GTT^AAC 4708 GTTAAC NaeI 787 GCCGGC GCC^GGC 1779 GCCGGC NcoI 3293 CCATGG C^CATGG 4400 CCATGG NdeI 1439 CATATG CA^TATG 4542 CATATG NgoMIV 787 GCCGGC G^CCGGC 1779 GCCGGC NheI 4393 GCTAGC G^CTAGC 5577 GCTAGC SbfI 4899 CCTGCAGG CCTGCA^GG 6498 CCTGCAGG ScaI 5262 AGTACT AGT^ACT 5980 AGTACT Sse8387I 4899 CCTGCAGG CCTGCA^GG 6498 CCTGCAGG SspBI 1169 TGTACA T^GTACA 6761 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 138 enzymes) # ######################################### AccII AccIII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII