##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AflII AgeI AhdI Aor51HI AscI
AspEI AviII BfrI BsaI BsiWI
BspLU11I BssSI Bst1107I BstBI BstZ17I
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV FseI FspI HpaI
MfeI MluI MunI NdeI NheI
NotI NspV PacI PaeR7I PinAI
PmeI PvuI RsrII SapI SbfI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AclI 666 AACGTT AA^CGTT
AseI 4401 ATTAAT AT^TAAT
AsnI 4401 ATTAAT AT^TAAT
AvrII 2821 CCTAGG C^CTAGG
BbeI 1 GGCGCC GGCGC^C
BcgI 5231 GCATGGGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5053 GGATAC GTATCC(6/5)
BclI 341 TGATCA T^GATCA
BglII 1064 AGATCT A^GATCT
BlnI 2821 CCTAGG C^CTAGG
BsaBI 4818 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 13 CGGTCG CG[GA][CT]^CG
BspDI 595 ATCGAT AT^CGAT
BspHI 2039 TCATGA T^CATGA
BsrBI 452 GAGCGG CCGCTC(-3/-3)
BssHII 46 GCGCGC G^CGCGC
ClaI 595 ATCGAT AT^CGAT
DraI 5742 TTTAAA TTT^AAA
DraIII 536 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 5289 ATGCAT ATGCA^T
FbaI 341 TGATCA T^GATCA
HindIII 1570 AAGCTT A^AGCTT
KasI 1 GGCGCC G^GCGCC
MamI 4818 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1 GGCGCC GG^CGCC
NcoI 2345 CCATGG C^CATGG
NruI 5868 TCGCGA TCG^CGA
NsiI 5289 ATGCAT ATGCA^T
NspI 856 GCATGC [GA]CATG^[CT]
PshBI 4401 ATTAAT AT^TAAT
Psp1406I 666 AACGTT AA^CGTT
RcaI 2039 TCATGA T^CATGA
SfoI 1 GGCGCC GGC^GCC
SphI 856 GCATGC GCATG^C
XcmI 3531 CCATTATGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AatII 2238 GACGTC GACGT^C
4558 GACGTC
AcsI 2788 GAATTC [GA]^AATT[CT]
3872 GAATTC
ApoI 2788 GAATTC [GA]^AATT[CT]
3872 GAATTC
BbrPI 232 CACGTG CAC^GTG
4430 CACGTG
Bsp1407I 3387 TGTACA T^GTACA
5852 TGTACA
BsrGI 3387 TGTACA T^GTACA
5852 TGTACA
EcoRI 2788 GAATTC G^AATTC
3872 GAATTC
KspI 5 CCGCGG CCGC^GG
160 CCGCGG
PflMI 708 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1082 CCAGCCGCTGG
PmaCI 232 CACGTG CAC^GTG
4430 CACGTG
PmlI 232 CACGTG CAC^GTG
4430 CACGTG
PshAI 2477 GACTACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4048 GACAACTGTC
SacII 5 CCGCGG CCGC^GG
160 CCGCGG
SalI 4055 GTCGAC G^TCGAC
4555 GTCGAC
ScaI 1457 AGTACT AGT^ACT
3814 AGTACT
SexAI 2061 ACCTGGT A^CC[AT]GGT
2868 ACCAGGT
SfiI 5486 GGCCCAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5495 GGCCACATTGGCC
SspBI 3387 TGTACA T^GTACA
5852 TGTACA
Van91I 708 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1082 CCAGCCGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 157 enzymes) #
#########################################
Acc65I AccI AccII AccIII AciI
AcyI AfaI AflIII AluI Alw44I
AlwI AlwNI AocI ApaI ApaLI
Asp700I Asp718I AspHI AspI AvaI
AvaII BalI BamHI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspEI BspMI BsrDI
BsrFI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PflFI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XbaI XhoII XmaCI
XmaI XmnI