##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AccIII AflII AgeI AscI
AseI AsnI AviII AvrII BbrPI
BfrI BlnI BsaAI BsaBI BseAI
BsiWI BspDI BspEI BssHII ClaI
CpoI DraI DraIII FseI FspI
HpaI KspI MamI MluI MroI
NaeI NgoMIV NheI NotI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI PvuI RsrII SacII SexAI
SfiI SgrAI SmaI SnaBI SpeI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 1807 GGTACC G^GTACC
Alw44I 2881 GTGCAC G^TGCAC
ApaLI 2881 GTGCAC G^TGCAC
Asp718I 1807 GGTACC G^GTACC
AvaI 98 CTCGGG C^[CT]CG[GA]G
BamHI 767 GGATCC G^GATCC
BbeI 4205 GGCGCC GGCGC^C
BbvCI 5779 GCTGAGG CCTCAGC(-5/-2)
BciVI 4311 GGATAC GTATCC(6/5)
BglI 5790 GCCATTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1018 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1018 GCTGAGC GC^T[ACGT]AGC
BsiEI 1891 CGGCCG CG[GA][CT]^CG
BsoBI 98 CTCGGG C^[CT]CG[GA]G
BssSI 1483 CTCGTG CACGAG(-5/-1)
Bst1107I 3484 GTATAC GTA^TAC
BstEII 5163 GGTCACC G^GT[ACGT]ACC
BstPI 5163 GGTCACC G^GT[ACGT]ACC
BstZ17I 3484 GTATAC GTA^TAC
CelII 1018 GCTGAGC GC^T[ACGT]AGC
EagI 1891 CGGCCG C^GGCCG
EclXI 1891 CGGCCG C^GGCCG
Eco52I 1891 CGGCCG C^GGCCG
EcoO65I 5163 GGTCACC G^GT[ACGT]ACC
EcoRI 3429 GAATTC G^AATTC
HindIII 1205 AAGCTT A^AGCTT
KasI 4205 GGCGCC G^GCGCC
KpnI 1807 GGTACC GGTAC^C
MfeI 697 CAATTG C^AATTG
MunI 697 CAATTG C^AATTG
NarI 4205 GGCGCC GG^CGCC
NruI 1575 TCGCGA TCG^CGA
SalI 1318 GTCGAC G^TCGAC
SfoI 4205 GGCGCC GGC^GCC
SphI 5021 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccII 1576 CGCG CG^CG
3396 CGCG
AclI 1415 AACGTT AA^CGTT
5234 AACGTT
AocI 56 CCTCAGG CC^T[ACGT]AGG
2725 CCTGAGG
Aor51HI 596 AGCGCT AGC^GCT
2485 AGCGCT
ApaI 3365 GGGCCC GGGCC^C
5463 GGGCCC
AspI 2571 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5764 GACTAGGTC
BanI 1807 GGTACC G^G[CT][GA]CC
4205 GGCGCC
BcgI 1578 CGAGGGTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2535 CGAAGGCTTTGC
BclI 431 TGATCA T^GATCA
4301 TGATCA
BglII 486 AGATCT A^GATCT
1713 AGATCT
BsaWI 764 ACCGGA [AT]^CCGG[AT]
2108 TCCGGT
BsmBI 3154 GAGACG CGTCTC(1/5)
5525 GAGACG
Bsp1407I 1817 TGTACA T^GTACA
3335 TGTACA
BspLU11I 1538 ACATGT A^CATGT
4980 ACATGT
BsrBI 2770 GAGCGG CCGCTC(-3/-3)
3763 GAGCGG
BsrFI 2585 GCCGGT [GA]^CCGG[CT]
4394 ACCGGC
BsrGI 1817 TGTACA T^GTACA
3335 TGTACA
BstBI 2605 TTCGAA TT^CGAA
2813 TTCGAA
BstUI 1576 CGCG CG^CG
3396 CGCG
Bsu36I 56 CCTCAGG CC^T[ACGT]AGG
2725 CCTGAGG
Cfr10I 2585 GCCGGT [GA]^CCGG[CT]
4394 ACCGGC
DrdI 128 GACAGCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1259 GACTCATTGGTC
Eco47III 596 AGCGCT AGC^GCT
2485 AGCGCT
Eco81I 56 CCTCAGG CC^T[ACGT]AGG
2725 CCTGAGG
EcoNI 957 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5064 CCTTAGTAAGG
FbaI 431 TGATCA T^GATCA
4301 TGATCA
MvnI 1576 CGCG CG^CG
3396 CGCG
NdeI 1001 CATATG CA^TATG
1382 CATATG
NspV 2605 TTCGAA TT^CGAA
2813 TTCGAA
PflFI 2571 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5764 GACTAGGTC
PshAI 1186 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1537 GACATGTGTC
Psp1406I 1415 AACGTT AA^CGTT
5234 AACGTT
SapI 4221 GCTCTTC GCTCTTC(1/4)
4809 GCTCTTC
SbfI 4401 CCTGCAGG CCTGCA^GG
4635 CCTGCAGG
SfuI 2605 TTCGAA TT^CGAA
2813 TTCGAA
Sse8387I 4401 CCTGCAGG CCTGCA^GG
4635 CCTGCAGG
SspBI 1817 TGTACA T^GTACA
3335 TGTACA
StuI 4185 AGGCCT AGG^CCT
5090 AGGCCT
Tth111I 2571 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5764 GACTAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
ApoI Asp700I AspEI AspHI AvaII
BalI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eam1105I
EarI EcoO109I EcoRII EcoRV EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI