################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## AatII AccIII AflII AgeI AscI AseI AsnI AviII AvrII BbrPI BfrI BlnI BsaAI BsaBI BseAI BsiWI BspDI BspEI BssHII ClaI CpoI DraI DraIII FseI FspI HpaI KspI MamI MluI MroI NaeI NgoMIV NheI NotI PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI PvuI RsrII SacII SexAI SfiI SgrAI SmaI SnaBI SpeI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### Acc65I 1807 GGTACC G^GTACC Alw44I 2881 GTGCAC G^TGCAC ApaLI 2881 GTGCAC G^TGCAC Asp718I 1807 GGTACC G^GTACC AvaI 98 CTCGGG C^[CT]CG[GA]G BamHI 767 GGATCC G^GATCC BbeI 4205 GGCGCC GGCGC^C BbvCI 5779 GCTGAGG CCTCAGC(-5/-2) BciVI 4311 GGATAC GTATCC(6/5) BglI 5790 GCCATTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1018 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1018 GCTGAGC GC^T[ACGT]AGC BsiEI 1891 CGGCCG CG[GA][CT]^CG BsoBI 98 CTCGGG C^[CT]CG[GA]G BssSI 1483 CTCGTG CACGAG(-5/-1) Bst1107I 3484 GTATAC GTA^TAC BstEII 5163 GGTCACC G^GT[ACGT]ACC BstPI 5163 GGTCACC G^GT[ACGT]ACC BstZ17I 3484 GTATAC GTA^TAC CelII 1018 GCTGAGC GC^T[ACGT]AGC EagI 1891 CGGCCG C^GGCCG EclXI 1891 CGGCCG C^GGCCG Eco52I 1891 CGGCCG C^GGCCG EcoO65I 5163 GGTCACC G^GT[ACGT]ACC EcoRI 3429 GAATTC G^AATTC HindIII 1205 AAGCTT A^AGCTT KasI 4205 GGCGCC G^GCGCC KpnI 1807 GGTACC GGTAC^C MfeI 697 CAATTG C^AATTG MunI 697 CAATTG C^AATTG NarI 4205 GGCGCC GG^CGCC NruI 1575 TCGCGA TCG^CGA SalI 1318 GTCGAC G^TCGAC SfoI 4205 GGCGCC GGC^GCC SphI 5021 GCATGC GCATG^C ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccII 1576 CGCG CG^CG 3396 CGCG AclI 1415 AACGTT AA^CGTT 5234 AACGTT AocI 56 CCTCAGG CC^T[ACGT]AGG 2725 CCTGAGG Aor51HI 596 AGCGCT AGC^GCT 2485 AGCGCT ApaI 3365 GGGCCC GGGCC^C 5463 GGGCCC AspI 2571 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 5764 GACTAGGTC BanI 1807 GGTACC G^G[CT][GA]CC 4205 GGCGCC BcgI 1578 CGAGGGTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2535 CGAAGGCTTTGC BclI 431 TGATCA T^GATCA 4301 TGATCA BglII 486 AGATCT A^GATCT 1713 AGATCT BsaWI 764 ACCGGA [AT]^CCGG[AT] 2108 TCCGGT BsmBI 3154 GAGACG CGTCTC(1/5) 5525 GAGACG Bsp1407I 1817 TGTACA T^GTACA 3335 TGTACA BspLU11I 1538 ACATGT A^CATGT 4980 ACATGT BsrBI 2770 GAGCGG CCGCTC(-3/-3) 3763 GAGCGG BsrFI 2585 GCCGGT [GA]^CCGG[CT] 4394 ACCGGC BsrGI 1817 TGTACA T^GTACA 3335 TGTACA BstBI 2605 TTCGAA TT^CGAA 2813 TTCGAA BstUI 1576 CGCG CG^CG 3396 CGCG Bsu36I 56 CCTCAGG CC^T[ACGT]AGG 2725 CCTGAGG Cfr10I 2585 GCCGGT [GA]^CCGG[CT] 4394 ACCGGC DrdI 128 GACAGCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1259 GACTCATTGGTC Eco47III 596 AGCGCT AGC^GCT 2485 AGCGCT Eco81I 56 CCTCAGG CC^T[ACGT]AGG 2725 CCTGAGG EcoNI 957 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5064 CCTTAGTAAGG FbaI 431 TGATCA T^GATCA 4301 TGATCA MvnI 1576 CGCG CG^CG 3396 CGCG NdeI 1001 CATATG CA^TATG 1382 CATATG NspV 2605 TTCGAA TT^CGAA 2813 TTCGAA PflFI 2571 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 5764 GACTAGGTC PshAI 1186 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1537 GACATGTGTC Psp1406I 1415 AACGTT AA^CGTT 5234 AACGTT SapI 4221 GCTCTTC GCTCTTC(1/4) 4809 GCTCTTC SbfI 4401 CCTGCAGG CCTGCA^GG 4635 CCTGCAGG SfuI 2605 TTCGAA TT^CGAA 2813 TTCGAA Sse8387I 4401 CCTGCAGG CCTGCA^GG 4635 CCTGCAGG SspBI 1817 TGTACA T^GTACA 3335 TGTACA StuI 4185 AGGCCT AGG^CCT 5090 AGGCCT Tth111I 2571 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 5764 GACTAGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 129 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AflIII AhdI AluI AlwI AlwNI ApoI Asp700I AspEI AspHI AvaII BalI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI EcoO109I EcoRII EcoRV EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI