##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
AscI Asp718I AspEI AspI BcgI
BciVI BglII BsaBI BseAI BsiWI
BspDI BspEI BstBI ClaI CpoI
Eam1105I EcoRI EcoRV EcoT22I HindIII
KpnI MamI MfeI MluI MroI
MunI NheI NruI NsiI NspV
PacI PflFI PinAI PmeI PshAI
Psp1406I PvuI RsrII SalI SapI
SbfI ScaI SexAI SfuI SnaBI
SphI Sse8387I SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 2808 GACGTC GACGT^C
AccI 5450 GTATAC GT^[AC][GT]AC
AcsI 3645 GAATTT [GA]^AATT[CT]
AflII 3045 CTTAAG C^TTAAG
Aor51HI 437 AGCGCT AGC^GCT
ApoI 3645 GAATTT [GA]^AATT[CT]
Asp700I 2829 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 3126 TGCGCA TGC^GCA
AvrII 1254 CCTAGG C^CTAGG
BbrPI 1560 CACGTG CAC^GTG
BclI 5390 TGATCA T^GATCA
BfrI 3045 CTTAAG C^TTAAG
BlnI 1254 CCTAGG C^CTAGG
BsaAI 1560 CACGTG [CT]AC^GT[GA]
BsaI 4483 GAGACC GGTCTC(1/5)
BsaWI 1982 TCCGGT [AT]^CCGG[AT]
BsmBI 3309 GAGACG CGTCTC(1/5)
BsrDI 4975 CATTGC GCAATG(2/0)
Bst1107I 5450 GTATAC GTA^TAC
BstEII 5148 GGTAACC G^GT[ACGT]ACC
BstPI 5148 GGTAACC G^GT[ACGT]ACC
BstZ17I 5450 GTATAC GTA^TAC
DraIII 4475 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2406 GACTCTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 437 AGCGCT AGC^GCT
EcoO65I 5148 GGTAACC G^GT[ACGT]ACC
FbaI 5390 TGATCA T^GATCA
FseI 3106 GGCCGGCC GGCCGG^CC
FspI 3126 TGCGCA TGC^GCA
HpaI 5378 GTTAAC GTT^AAC
NdeI 2645 CATATG CA^TATG
NotI 538 GCGGCCGC GC^GGCCGC
PflMI 1291 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1560 CACGTG CAC^GTG
PmlI 1560 CACGTG CAC^GTG
SfiI 531 GGCCCCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 325 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 226 CCCGGG CCC^GGG
StuI 3778 AGGCCT AGG^CCT
Van91I 1291 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 226 CCCGGG C^CCGGG
XmaI 226 CCCGGG C^CCGGG
XmnI 2829 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 4379 ACATGT A^C[GA][CT]GT
5220 ACATGT
AseI 1162 ATTAAT AT^TAAT
5417 ATTAAT
AsnI 1162 ATTAAT AT^TAAT
5417 ATTAAT
BbvCI 2106 CCTCAGC CCTCAGC(-5/-2)
2395 CCTCAGC
BlpI 2221 GCTGAGC GC^T[ACGT]AGC
3475 GCTCAGC
Bpu1102I 2221 GCTGAGC GC^T[ACGT]AGC
3475 GCTCAGC
BsmI 2763 GAATGC GAATGC(1/-1)
2786 GAATGC
Bsp1407I 4036 TGTACA T^GTACA
4062 TGTACA
BspHI 597 TCATGA T^CATGA
1670 TCATGA
BspLU11I 4379 ACATGT A^CATGT
5220 ACATGT
BsrGI 4036 TGTACA T^GTACA
4062 TGTACA
BssHII 462 GCGCGC G^CGCGC
544 GCGCGC
CelII 2221 GCTGAGC GC^T[ACGT]AGC
3475 GCTCAGC
HincII 1331 GTCAAC GT[CT]^[GA]AC
5378 GTTAAC
HindII 1331 GTCAAC GT[CT]^[GA]AC
5378 GTTAAC
KspI 247 CCGCGG CCGC^GG
295 CCGCGG
PaeR7I 406 CTCGAG C^TCGAG
2384 CTCGAG
PshBI 1162 ATTAAT AT^TAAT
5417 ATTAAT
RcaI 597 TCATGA T^CATGA
1670 TCATGA
SacII 247 CCGCGG CCGC^GG
295 CCGCGG
SspBI 4036 TGTACA T^GTACA
4062 TGTACA
SspI 1165 AATATT AAT^ATT
5501 AATATT
XhoI 406 CTCGAG C^TCGAG
2384 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BalI
BamHI BanI BanII BbeI BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EagI EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
SpeI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII