################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## Acc65I AccIII AclI AgeI AhdI AscI Asp718I AspEI AspI BcgI BciVI BglII BsaBI BseAI BsiWI BspDI BspEI BstBI ClaI CpoI Eam1105I EcoRI EcoRV EcoT22I HindIII KpnI MamI MfeI MluI MroI MunI NheI NruI NsiI NspV PacI PflFI PinAI PmeI PshAI Psp1406I PvuI RsrII SalI SapI SbfI ScaI SexAI SfuI SnaBI SphI Sse8387I SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 2808 GACGTC GACGT^C AccI 5450 GTATAC GT^[AC][GT]AC AcsI 3645 GAATTT [GA]^AATT[CT] AflII 3045 CTTAAG C^TTAAG Aor51HI 437 AGCGCT AGC^GCT ApoI 3645 GAATTT [GA]^AATT[CT] Asp700I 2829 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 3126 TGCGCA TGC^GCA AvrII 1254 CCTAGG C^CTAGG BbrPI 1560 CACGTG CAC^GTG BclI 5390 TGATCA T^GATCA BfrI 3045 CTTAAG C^TTAAG BlnI 1254 CCTAGG C^CTAGG BsaAI 1560 CACGTG [CT]AC^GT[GA] BsaI 4483 GAGACC GGTCTC(1/5) BsaWI 1982 TCCGGT [AT]^CCGG[AT] BsmBI 3309 GAGACG CGTCTC(1/5) BsrDI 4975 CATTGC GCAATG(2/0) Bst1107I 5450 GTATAC GTA^TAC BstEII 5148 GGTAACC G^GT[ACGT]ACC BstPI 5148 GGTAACC G^GT[ACGT]ACC BstZ17I 5450 GTATAC GTA^TAC DraIII 4475 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2406 GACTCTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 437 AGCGCT AGC^GCT EcoO65I 5148 GGTAACC G^GT[ACGT]ACC FbaI 5390 TGATCA T^GATCA FseI 3106 GGCCGGCC GGCCGG^CC FspI 3126 TGCGCA TGC^GCA HpaI 5378 GTTAAC GTT^AAC NdeI 2645 CATATG CA^TATG NotI 538 GCGGCCGC GC^GGCCGC PflMI 1291 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1560 CACGTG CAC^GTG PmlI 1560 CACGTG CAC^GTG SfiI 531 GGCCCCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 325 CGCCGGCG C[GA]^CCGG[CT]G SmaI 226 CCCGGG CCC^GGG StuI 3778 AGGCCT AGG^CCT Van91I 1291 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 226 CCCGGG C^CCGGG XmaI 226 CCCGGG C^CCGGG XmnI 2829 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AflIII 4379 ACATGT A^C[GA][CT]GT 5220 ACATGT AseI 1162 ATTAAT AT^TAAT 5417 ATTAAT AsnI 1162 ATTAAT AT^TAAT 5417 ATTAAT BbvCI 2106 CCTCAGC CCTCAGC(-5/-2) 2395 CCTCAGC BlpI 2221 GCTGAGC GC^T[ACGT]AGC 3475 GCTCAGC Bpu1102I 2221 GCTGAGC GC^T[ACGT]AGC 3475 GCTCAGC BsmI 2763 GAATGC GAATGC(1/-1) 2786 GAATGC Bsp1407I 4036 TGTACA T^GTACA 4062 TGTACA BspHI 597 TCATGA T^CATGA 1670 TCATGA BspLU11I 4379 ACATGT A^CATGT 5220 ACATGT BsrGI 4036 TGTACA T^GTACA 4062 TGTACA BssHII 462 GCGCGC G^CGCGC 544 GCGCGC CelII 2221 GCTGAGC GC^T[ACGT]AGC 3475 GCTCAGC HincII 1331 GTCAAC GT[CT]^[GA]AC 5378 GTTAAC HindII 1331 GTCAAC GT[CT]^[GA]AC 5378 GTTAAC KspI 247 CCGCGG CCGC^GG 295 CCGCGG PaeR7I 406 CTCGAG C^TCGAG 2384 CTCGAG PshBI 1162 ATTAAT AT^TAAT 5417 ATTAAT RcaI 597 TCATGA T^CATGA 1670 TCATGA SacII 247 CCGCGG CCGC^GG 295 CCGCGG SspBI 4036 TGTACA T^GTACA 4062 TGTACA SspI 1165 AATATT AAT^ATT 5501 AATATT XhoI 406 CTCGAG C^TCGAG 2384 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 137 enzymes) # ######################################### AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI AspHI AvaI AvaII BalI BamHI BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI SpeI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII