##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AflII AgeI AhdI
Aor51HI AscI Asp700I Asp718I AspEI
AspI AviII BbeI BbrPI BbvCI
BfrI BsiWI BsmBI BsrFI BssHII
BssSI BstBI Cfr10I CpoI DraIII
Eam1105I Eco47III FseI FspI HpaI
KasI KpnI KspI MluI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PacI PflFI PinAI PmaCI
PmlI RsrII SacII SalI SfiI
SfoI SfuI SgrAI SmaI SnaBI
StuI SwaI Tth111I XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccIII 347 TCCGGA T^CCGGA
AclI 1873 AACGTT AA^CGTT
AcyI 3867 GACGCC G[GA]^CG[CT]C
AocI 2920 CCTCAGG CC^T[ACGT]AGG
ApaI 4469 GGGCCC GGGCC^C
AseI 2604 ATTAAT AT^TAAT
AsnI 2604 ATTAAT AT^TAAT
AvrII 3985 CCTAGG C^CTAGG
BamHI 4026 GGATCC G^GATCC
BanI 888 GGTGCC G^G[CT][GA]CC
BcgI 3796 GCATTGAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 216 CCCGG CC^[GC]GG
BglI 2023 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 5307 AGATCT A^GATCT
BlnI 3985 CCTAGG C^CTAGG
BsaAI 732 CACGTA [CT]AC^GT[GA]
BsaBI 4388 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3867 GACGCC G[GA]^CG[CT]C
BseAI 347 TCCGGA T^CCGGA
BspDI 184 ATCGAT AT^CGAT
BspEI 347 TCCGGA T^CCGGA
BspHI 5085 TCATGA T^CATGA
BspMI 1186 GCAGGT ACCTGC(4/8)
BstXI 50 CCACGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2920 CCTCAGG CC^T[ACGT]AGG
ClaI 184 ATCGAT AT^CGAT
DraII 3383 GGGTCCT [GA]G^G[ACGT]CC[CT]
Eco81I 2920 CCTCAGG CC^T[ACGT]AGG
EcoNI 292 CCTCTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 3383 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 3511 GAATTC G^AATTC
EcoT22I 3817 ATGCAT ATGCA^T
HaeII 3373 AGCGCC [GA]GCGC^[CT]
Hin1I 3867 GACGCC G[GA]^CG[CT]C
MamI 4388 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 347 TCCGGA T^CCGGA
NciI 216 CCCGG CC^[GC]GG
NcoI 1968 CCATGG C^CATGG
NheI 5951 GCTAGC G^CTAGC
NsiI 3817 ATGCAT ATGCA^T
PaeR7I 3452 CTCGAG C^TCGAG
PmeI 3681 GTTTAAAC GTTT^AAAC
PpuMI 3383 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 2604 ATTAAT AT^TAAT
Psp1406I 1873 AACGTT AA^CGTT
RcaI 5085 TCATGA T^CATGA
SbfI 150 CCTGCAGG CCTGCA^GG
SexAI 303 ACCAGGT A^CC[AT]GGT
SpeI 4607 ACTAGT A^CTAGT
Sse8387I 150 CCTGCAGG CCTGCA^GG
XbaI 2256 TCTAGA T^CTAGA
XcmI 2271 CCAGTTTTCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3452 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 1 CGCG CG^CG
171 CGCG
Alw44I 1318 GTGCAC G^TGCAC
3745 GTGCAC
ApaLI 1318 GTGCAC G^TGCAC
3745 GTGCAC
BciVI 3024 GGATAC GTATCC(6/5)
4073 GTATCC
BsaI 4788 GAGACC GGTCTC(1/5)
5930 GGTCTC
BsaWI 347 TCCGGA [AT]^CCGG[AT]
1265 TCCGGT
Bsp1407I 642 TGTACA T^GTACA
1563 TGTACA
BsrBI 3377 CCGCTC CCGCTC(-3/-3)
12 GAGCGG
BsrGI 642 TGTACA T^GTACA
1563 TGTACA
Bst1107I 969 GTATAC GTA^TAC
4983 GTATAC
BstAPI 1572 GCACATGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2602 GCATTAATTGC
BstEII 490 GGTGACC G^GT[ACGT]ACC
1268 GGTTACC
BstPI 490 GGTGACC G^GT[ACGT]ACC
1268 GGTTACC
BstUI 1 CGCG CG^CG
171 CGCG
BstZ17I 969 GTATAC GTA^TAC
4983 GTATAC
CfoI 3374 GCGC GCG^C
3719 GCGC
DrdI 2584 GACAAAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4538 GACACAGCAGTC
EagI 137 CGGCCG C^GGCCG
1237 CGGCCG
EclXI 137 CGGCCG C^GGCCG
1237 CGGCCG
Eco52I 137 CGGCCG C^GGCCG
1237 CGGCCG
EcoO65I 490 GGTGACC G^GT[ACGT]ACC
1268 GGTTACC
EcoRV 3763 GATATC GAT^ATC
4172 GATATC
FauI 363 CCCGC CCCGC(4/6)
172 GCGGG
HhaI 3374 GCGC GCG^C
3719 GCGC
HinP1I 3374 GCGC G^CGC
3719 GCGC
MfeI 1118 CAATTG C^AATTG
2186 CAATTG
MunI 1118 CAATTG C^AATTG
2186 CAATTG
MvnI 1 CGCG CG^CG
171 CGCG
PshAI 1251 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2677 GACACCTGTC
PvuI 186 CGATCG CGAT^CG
976 CGATCG
SapI 3195 GCTCTTC GCTCTTC(1/4)
4011 GCTCTTC
ScaI 3587 AGTACT AGT^ACT
5994 AGTACT
SmlI 3452 CTCGAG C^T[CT][GA]AG
3832 CTCAAG
SphI 686 GCATGC GCATG^C
3819 GCATGC
SspBI 642 TGTACA T^GTACA
1563 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanII BbsI
BbvI BclI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspLU11I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII Cfr13I
DdeI DpnII DraI DsaI EaeI
EarI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HgaI HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII