################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AatII Acc65I AflII AgeI AhdI Aor51HI AscI Asp700I Asp718I AspEI AspI AviII BbeI BbrPI BbvCI BfrI BsiWI BsmBI BsrFI BssHII BssSI BstBI Cfr10I CpoI DraIII Eam1105I Eco47III FseI FspI HpaI KasI KpnI KspI MluI NaeI NarI NdeI NgoMIV NotI NruI NspV PacI PflFI PinAI PmaCI PmlI RsrII SacII SalI SfiI SfoI SfuI SgrAI SmaI SnaBI StuI SwaI Tth111I XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccIII 347 TCCGGA T^CCGGA AclI 1873 AACGTT AA^CGTT AcyI 3867 GACGCC G[GA]^CG[CT]C AocI 2920 CCTCAGG CC^T[ACGT]AGG ApaI 4469 GGGCCC GGGCC^C AseI 2604 ATTAAT AT^TAAT AsnI 2604 ATTAAT AT^TAAT AvrII 3985 CCTAGG C^CTAGG BamHI 4026 GGATCC G^GATCC BanI 888 GGTGCC G^G[CT][GA]CC BcgI 3796 GCATTGAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 216 CCCGG CC^[GC]GG BglI 2023 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 5307 AGATCT A^GATCT BlnI 3985 CCTAGG C^CTAGG BsaAI 732 CACGTA [CT]AC^GT[GA] BsaBI 4388 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 3867 GACGCC G[GA]^CG[CT]C BseAI 347 TCCGGA T^CCGGA BspDI 184 ATCGAT AT^CGAT BspEI 347 TCCGGA T^CCGGA BspHI 5085 TCATGA T^CATGA BspMI 1186 GCAGGT ACCTGC(4/8) BstXI 50 CCACGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2920 CCTCAGG CC^T[ACGT]AGG ClaI 184 ATCGAT AT^CGAT DraII 3383 GGGTCCT [GA]G^G[ACGT]CC[CT] Eco81I 2920 CCTCAGG CC^T[ACGT]AGG EcoNI 292 CCTCTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 3383 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 3511 GAATTC G^AATTC EcoT22I 3817 ATGCAT ATGCA^T HaeII 3373 AGCGCC [GA]GCGC^[CT] Hin1I 3867 GACGCC G[GA]^CG[CT]C MamI 4388 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 347 TCCGGA T^CCGGA NciI 216 CCCGG CC^[GC]GG NcoI 1968 CCATGG C^CATGG NheI 5951 GCTAGC G^CTAGC NsiI 3817 ATGCAT ATGCA^T PaeR7I 3452 CTCGAG C^TCGAG PmeI 3681 GTTTAAAC GTTT^AAAC PpuMI 3383 GGGTCCT [GA]G^G[AT]CC[CT] PshBI 2604 ATTAAT AT^TAAT Psp1406I 1873 AACGTT AA^CGTT RcaI 5085 TCATGA T^CATGA SbfI 150 CCTGCAGG CCTGCA^GG SexAI 303 ACCAGGT A^CC[AT]GGT SpeI 4607 ACTAGT A^CTAGT Sse8387I 150 CCTGCAGG CCTGCA^GG XbaI 2256 TCTAGA T^CTAGA XcmI 2271 CCAGTTTTCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 3452 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccII 1 CGCG CG^CG 171 CGCG Alw44I 1318 GTGCAC G^TGCAC 3745 GTGCAC ApaLI 1318 GTGCAC G^TGCAC 3745 GTGCAC BciVI 3024 GGATAC GTATCC(6/5) 4073 GTATCC BsaI 4788 GAGACC GGTCTC(1/5) 5930 GGTCTC BsaWI 347 TCCGGA [AT]^CCGG[AT] 1265 TCCGGT Bsp1407I 642 TGTACA T^GTACA 1563 TGTACA BsrBI 3377 CCGCTC CCGCTC(-3/-3) 12 GAGCGG BsrGI 642 TGTACA T^GTACA 1563 TGTACA Bst1107I 969 GTATAC GTA^TAC 4983 GTATAC BstAPI 1572 GCACATGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2602 GCATTAATTGC BstEII 490 GGTGACC G^GT[ACGT]ACC 1268 GGTTACC BstPI 490 GGTGACC G^GT[ACGT]ACC 1268 GGTTACC BstUI 1 CGCG CG^CG 171 CGCG BstZ17I 969 GTATAC GTA^TAC 4983 GTATAC CfoI 3374 GCGC GCG^C 3719 GCGC DrdI 2584 GACAAAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4538 GACACAGCAGTC EagI 137 CGGCCG C^GGCCG 1237 CGGCCG EclXI 137 CGGCCG C^GGCCG 1237 CGGCCG Eco52I 137 CGGCCG C^GGCCG 1237 CGGCCG EcoO65I 490 GGTGACC G^GT[ACGT]ACC 1268 GGTTACC EcoRV 3763 GATATC GAT^ATC 4172 GATATC FauI 363 CCCGC CCCGC(4/6) 172 GCGGG HhaI 3374 GCGC GCG^C 3719 GCGC HinP1I 3374 GCGC G^CGC 3719 GCGC MfeI 1118 CAATTG C^AATTG 2186 CAATTG MunI 1118 CAATTG C^AATTG 2186 CAATTG MvnI 1 CGCG CG^CG 171 CGCG PshAI 1251 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2677 GACACCTGTC PvuI 186 CGATCG CGAT^CG 976 CGATCG SapI 3195 GCTCTTC GCTCTTC(1/4) 4011 GCTCTTC ScaI 3587 AGTACT AGT^ACT 5994 AGTACT SmlI 3452 CTCGAG C^T[CT][GA]AG 3832 CTCAAG SphI 686 GCATGC GCATG^C 3819 GCATGC SspBI 642 TGTACA T^GTACA 1563 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanII BbsI BbvI BclI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII Cfr13I DdeI DpnII DraI DsaI EaeI EarI EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HgaI HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII