##################################
# NO CUT ENZYMES ( 35 enzymes) #
##################################
AatII AclI AflII AscI AvrII
BclI BfrI BlnI BsaBI BsiWI
Bst1107I BstZ17I CpoI DraI EcoRI
FbaI FseI MamI MluI NheI
NotI NruI PacI PmeI Psp1406I
PvuI RsrII SapI ScaI SexAI
SfiI SgrAI SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 903 GGTACC G^GTACC
AccIII 463 TCCGGA T^CCGGA
AgeI 2230 ACCGGT A^CCGGT
Aor51HI 733 AGCGCT AGC^GCT
AseI 666 ATTAAT AT^TAAT
AsnI 666 ATTAAT AT^TAAT
Asp718I 903 GGTACC G^GTACC
AspI 104 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3792 GGATCC G^GATCC
BbeI 2055 GGCGCC GGCGC^C
BglII 4814 AGATCT A^GATCT
BseAI 463 TCCGGA T^CCGGA
BsmBI 2695 GAGACG CGTCTC(1/5)
BspEI 463 TCCGGA T^CCGGA
BspLU11I 3177 ACATGT A^CATGT
BssHII 6277 GCGCGC G^CGCGC
BssSI 1500 CACGAG CACGAG(-5/-1)
BstBI 4638 TTCGAA TT^CGAA
BstEII 101 GGTGACC G^GT[ACGT]ACC
BstPI 101 GGTGACC G^GT[ACGT]ACC
DrdI 6339 GACAGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 733 AGCGCT AGC^GCT
EcoO65I 101 GGTGACC G^GT[ACGT]ACC
HpaI 4998 GTTAAC GTT^AAC
KasI 2055 GGCGCC G^GCGCC
KpnI 903 GGTACC GGTAC^C
KspI 1092 CCGCGG CCGC^GG
MfeI 1210 CAATTG C^AATTG
MroI 463 TCCGGA T^CCGGA
MunI 1210 CAATTG C^AATTG
NarI 2055 GGCGCC GG^CGCC
NspV 4638 TTCGAA TT^CGAA
PaeR7I 2689 CTCGAG C^TCGAG
PflFI 104 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 132 CCAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2230 ACCGGT A^CCGGT
PshBI 666 ATTAAT AT^TAAT
SacI 3825 GAGCTC GAGCT^C
SacII 1092 CCGCGG CCGC^GG
SalI 3738 GTCGAC G^TCGAC
SfoI 2055 GGCGCC GGC^GCC
SfuI 4638 TTCGAA TT^CGAA
SphI 6219 GCATGC GCATG^C
SspI 3312 AATATT AAT^ATT
StuI 2770 AGGCCT AGG^CCT
Tth111I 104 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 132 CCAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4854 TCTAGA T^CTAGA
XhoI 2689 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 3738 GTCGAC GT^[AC][GT]AC
5403 GTAGAC
AocI 343 CCTCAGG CC^T[ACGT]AGG
4787 CCTCAGG
AviII 1776 TGCGCA TGC^GCA
2541 TGCGCA
BalI 3878 TGGCCA TGG^CCA
5321 TGGCCA
BbvCI 6298 CCTCAGC CCTCAGC(-5/-2)
6587 GCTGAGG
BlpI 581 GCTCAGC GC^T[ACGT]AGC
6497 GCTCAGC
Bpu1102I 581 GCTCAGC GC^T[ACGT]AGC
6497 GCTCAGC
Bsp1407I 1653 TGTACA T^GTACA
2523 TGTACA
BspHI 1384 TCATGA T^CATGA
4430 TCATGA
BsrBI 1376 GAGCGG CCGCTC(-3/-3)
3750 GAGCGG
BsrGI 1653 TGTACA T^GTACA
2523 TGTACA
BstXI 2632 CCACTGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3724 CCATGTGCATGG
Bsu36I 343 CCTCAGG CC^T[ACGT]AGG
4787 CCTCAGG
CelII 581 GCTCAGC GC^T[ACGT]AGC
6497 GCTCAGC
DraIII 1037 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1697 CACCCAGTG
EagI 908 CGGCCG C^GGCCG
3571 CGGCCG
EclXI 908 CGGCCG C^GGCCG
3571 CGGCCG
Eco52I 908 CGGCCG C^GGCCG
3571 CGGCCG
Eco81I 343 CCTCAGG CC^T[ACGT]AGG
4787 CCTCAGG
EcoT22I 2150 ATGCAT ATGCA^T
4736 ATGCAT
FspI 1776 TGCGCA TGC^GCA
2541 TGCGCA
MluNI 3878 TGGCCA TGG^CCA
5321 TGGCCA
MscI 3878 TGGCCA TGG^CCA
5321 TGGCCA
NaeI 3573 GCCGGC GCC^GGC
6727 GCCGGC
NgoMIV 3573 GCCGGC G^CCGGC
6727 GCCGGC
NsiI 2150 ATGCAT ATGCA^T
4736 ATGCAT
PshAI 2697 GACGGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5535 GACGAATGTC
RcaI 1384 TCATGA T^CATGA
4430 TCATGA
SbfI 529 CCTGCAGG CCTGCA^GG
5501 CCTGCAGG
Sse8387I 529 CCTGCAGG CCTGCA^GG
5501 CCTGCAGG
SspBI 1653 TGTACA T^GTACA
2523 TGTACA
XcmI 974 CCACCCTGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4329 CCAGGCGAGGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI Alw44I AlwI
AlwNI ApaI ApaLI ApoI Asp700I
AspEI AspHI AvaI AvaII BanI
BanII BbrPI BbsI BbvI BcgI
BciVI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspDI BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I ClaI DdeI
DpnII DraII DsaI EaeI Eam1105I
EarI EcoNI EcoO109I EcoRII EcoRV
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeI NdeII NlaIII NlaIV NspI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI XmnI