################################## # NO CUT ENZYMES ( 35 enzymes) # ################################## AatII AclI AflII AscI AvrII BclI BfrI BlnI BsaBI BsiWI Bst1107I BstZ17I CpoI DraI EcoRI FbaI FseI MamI MluI NheI NotI NruI PacI PmeI Psp1406I PvuI RsrII SapI ScaI SexAI SfiI SgrAI SnaBI SpeI SwaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 903 GGTACC G^GTACC AccIII 463 TCCGGA T^CCGGA AgeI 2230 ACCGGT A^CCGGT Aor51HI 733 AGCGCT AGC^GCT AseI 666 ATTAAT AT^TAAT AsnI 666 ATTAAT AT^TAAT Asp718I 903 GGTACC G^GTACC AspI 104 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 3792 GGATCC G^GATCC BbeI 2055 GGCGCC GGCGC^C BglII 4814 AGATCT A^GATCT BseAI 463 TCCGGA T^CCGGA BsmBI 2695 GAGACG CGTCTC(1/5) BspEI 463 TCCGGA T^CCGGA BspLU11I 3177 ACATGT A^CATGT BssHII 6277 GCGCGC G^CGCGC BssSI 1500 CACGAG CACGAG(-5/-1) BstBI 4638 TTCGAA TT^CGAA BstEII 101 GGTGACC G^GT[ACGT]ACC BstPI 101 GGTGACC G^GT[ACGT]ACC DrdI 6339 GACAGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 733 AGCGCT AGC^GCT EcoO65I 101 GGTGACC G^GT[ACGT]ACC HpaI 4998 GTTAAC GTT^AAC KasI 2055 GGCGCC G^GCGCC KpnI 903 GGTACC GGTAC^C KspI 1092 CCGCGG CCGC^GG MfeI 1210 CAATTG C^AATTG MroI 463 TCCGGA T^CCGGA MunI 1210 CAATTG C^AATTG NarI 2055 GGCGCC GG^CGCC NspV 4638 TTCGAA TT^CGAA PaeR7I 2689 CTCGAG C^TCGAG PflFI 104 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 132 CCAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 2230 ACCGGT A^CCGGT PshBI 666 ATTAAT AT^TAAT SacI 3825 GAGCTC GAGCT^C SacII 1092 CCGCGG CCGC^GG SalI 3738 GTCGAC G^TCGAC SfoI 2055 GGCGCC GGC^GCC SfuI 4638 TTCGAA TT^CGAA SphI 6219 GCATGC GCATG^C SspI 3312 AATATT AAT^ATT StuI 2770 AGGCCT AGG^CCT Tth111I 104 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 132 CCAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 4854 TCTAGA T^CTAGA XhoI 2689 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 3738 GTCGAC GT^[AC][GT]AC 5403 GTAGAC AocI 343 CCTCAGG CC^T[ACGT]AGG 4787 CCTCAGG AviII 1776 TGCGCA TGC^GCA 2541 TGCGCA BalI 3878 TGGCCA TGG^CCA 5321 TGGCCA BbvCI 6298 CCTCAGC CCTCAGC(-5/-2) 6587 GCTGAGG BlpI 581 GCTCAGC GC^T[ACGT]AGC 6497 GCTCAGC Bpu1102I 581 GCTCAGC GC^T[ACGT]AGC 6497 GCTCAGC Bsp1407I 1653 TGTACA T^GTACA 2523 TGTACA BspHI 1384 TCATGA T^CATGA 4430 TCATGA BsrBI 1376 GAGCGG CCGCTC(-3/-3) 3750 GAGCGG BsrGI 1653 TGTACA T^GTACA 2523 TGTACA BstXI 2632 CCACTGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3724 CCATGTGCATGG Bsu36I 343 CCTCAGG CC^T[ACGT]AGG 4787 CCTCAGG CelII 581 GCTCAGC GC^T[ACGT]AGC 6497 GCTCAGC DraIII 1037 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG 1697 CACCCAGTG EagI 908 CGGCCG C^GGCCG 3571 CGGCCG EclXI 908 CGGCCG C^GGCCG 3571 CGGCCG Eco52I 908 CGGCCG C^GGCCG 3571 CGGCCG Eco81I 343 CCTCAGG CC^T[ACGT]AGG 4787 CCTCAGG EcoT22I 2150 ATGCAT ATGCA^T 4736 ATGCAT FspI 1776 TGCGCA TGC^GCA 2541 TGCGCA MluNI 3878 TGGCCA TGG^CCA 5321 TGGCCA MscI 3878 TGGCCA TGG^CCA 5321 TGGCCA NaeI 3573 GCCGGC GCC^GGC 6727 GCCGGC NgoMIV 3573 GCCGGC G^CCGGC 6727 GCCGGC NsiI 2150 ATGCAT ATGCA^T 4736 ATGCAT PshAI 2697 GACGGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5535 GACGAATGTC RcaI 1384 TCATGA T^CATGA 4430 TCATGA SbfI 529 CCTGCAGG CCTGCA^GG 5501 CCTGCAGG Sse8387I 529 CCTGCAGG CCTGCA^GG 5501 CCTGCAGG SspBI 1653 TGTACA T^GTACA 2523 TGTACA XcmI 974 CCACCCTGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4329 CCAGGCGAGGGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 142 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AhdI AluI Alw44I AlwI AlwNI ApaI ApaLI ApoI Asp700I AspEI AspHI AvaI AvaII BanI BanII BbrPI BbsI BbvI BcgI BciVI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspDI BspMI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I ClaI DdeI DpnII DraII DsaI EaeI Eam1105I EarI EcoNI EcoO109I EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI XmnI