##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI ApaI AscI Asp718I AviII
BamHI BbeI BcgI BfrI BglI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BsrGI BssHII BstBI
BstUI ClaI CpoI EagI EclXI
Eco52I FseI FspI KasI KpnI
KspI MfeI MluI MroI MunI
MvnI NarI NdeI NotI NruI
NspV PacI PinAI PmeI PshAI
Psp1406I PvuI RsrII SacII SalI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SspBI StuI SwaI XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 5770 GACGTC GACGT^C
AhdI 5110 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5630 GTGCAC G^TGCAC
Aor51HI 5824 AGCGCT AGC^GCT
ApaLI 5630 GTGCAC G^TGCAC
AseI 1007 ATTAAT AT^TAAT
AsnI 1007 ATTAAT AT^TAAT
AspEI 5110 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 5873 CACGTG CAC^GTG
BclI 2525 TGATCA T^GATCA
BsaAI 5873 CACGTG [CT]AC^GT[GA]
BsaI 1033 GAGACC GGTCTC(1/5)
BsaWI 5766 ACCGGA [AT]^CCGG[AT]
BspHI 5814 TCATGA T^CATGA
BsrFI 7 GCCGGC [GA]^CCGG[CT]
BssSI 1984 CACGAG CACGAG(-5/-1)
Bst1107I 5130 GTATAC GTA^TAC
BstEII 3466 GGTCACC G^GT[ACGT]ACC
BstPI 3466 GGTCACC G^GT[ACGT]ACC
BstZ17I 5130 GTATAC GTA^TAC
Cfr10I 7 GCCGGC [GA]^CCGG[CT]
Eam1105I 5110 GACCCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5824 AGCGCT AGC^GCT
EcoO65I 3466 GGTCACC G^GT[ACGT]ACC
EcoT22I 1350 ATGCAT ATGCA^T
FbaI 2525 TGATCA T^GATCA
HincII 5676 GTTAAC GT[CT]^[GA]AC
HindII 5676 GTTAAC GT[CT]^[GA]AC
HpaI 5676 GTTAAC GTT^AAC
NaeI 7 GCCGGC GCC^GGC
NgoMIV 7 GCCGGC G^CCGGC
NheI 5465 GCTAGC G^CTAGC
NsiI 1350 ATGCAT ATGCA^T
PaeR7I 315 CTCGAG C^TCGAG
PflMI 272 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 5873 CACGTG CAC^GTG
PmlI 5873 CACGTG CAC^GTG
PshBI 1007 ATTAAT AT^TAAT
RcaI 5814 TCATGA T^CATGA
SbfI 4093 CCTGCAGG CCTGCA^GG
Sse8387I 4093 CCTGCAGG CCTGCA^GG
SspI 3106 AATATT AAT^ATT
Van91I 272 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1070 CCAGCACCAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 315 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 152 GATCC GGATC(4/5)
2795 GATCC
AspI 804 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3451 GACACAGTC
AvaI 237 CTCGGG C^[CT]CG[GA]G
315 CTCGAG
AvrII 3967 CCTAGG C^CTAGG
4784 CCTAGG
BalI 4109 TGGCCA TGG^CCA
4996 TGGCCA
BciVI 50 GGATAC GTATCC(6/5)
3893 GGATAC
BcnI 397 CCCGG CC^[GC]GG
604 CCCGG
BlnI 3967 CCTAGG C^CTAGG
4784 CCTAGG
BsaBI 552 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
611 GATCTATATC
BsoBI 237 CTCGGG C^[CT]CG[GA]G
315 CTCGAG
BsrBI 4038 CCGCTC CCGCTC(-3/-3)
1888 GAGCGG
EaeI 4109 TGGCCA [CT]^GGCC[GA]
4996 TGGCCA
EcoNI 957 CCTTCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4473 CCTTCTTGAGG
EcoRI 2836 GAATTC G^AATTC
3486 GAATTC
MamI 552 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
611 GATCTATATC
MluNI 4109 TGGCCA TGG^CCA
4996 TGGCCA
MscI 4109 TGGCCA TGG^CCA
4996 TGGCCA
NciI 397 CCCGG CC^[GC]GG
604 CCCGG
PflFI 804 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3451 GACACAGTC
SapI 773 GAAGAGC GCTCTTC(1/4)
4467 GCTCTTC
ScaI 1367 AGTACT AGT^ACT
2087 AGTACT
SpeI 2898 ACTAGT A^CTAGT
3316 ACTAGT
Tth111I 804 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3451 GACACAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwNI AocI ApoI
Asp700I AspHI AvaII BanI BanII
BbsI BbvCI BbvI BfaI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspLU11I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DrdI DsaI EarI Eco81I
EcoO109I EcoRII EcoRV EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI