##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AccII AccIII Alw44I Aor51HI
ApaLI AscI Asp700I Asp718I AviII
AvrII BalI BbeI BglI BglII
BlnI BsaBI BseAI BspEI BssHII
Bst1107I BstUI BstZ17I CpoI Eco47III
FspI KasI KpnI KspI MamI
MluI MluNI MroI MscI MvnI
NarI NotI NruI PacI PmeI
PshAI PvuI RsrII SacII SalI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 2852 GACGTC GACGT^C
AclI 1380 AACGTT AA^CGTT
AflII 1823 CTTAAG C^TTAAG
AgeI 927 ACCGGT A^CCGGT
AhdI 1172 GACCCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4235 CCTCAGG CC^T[ACGT]AGG
ApaI 5600 GGGCCC GGGCC^C
AseI 1427 ATTAAT AT^TAAT
AsnI 1427 ATTAAT AT^TAAT
AspEI 1172 GACCCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4762 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 4240 GGATCC G^GATCC
BcgI 742 CGAGAACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1823 CTTAAG C^TTAAG
BsiWI 2487 CGTACG C^GTACG
BsmBI 2854 CGTCTC CGTCTC(1/5)
BstEII 6049 GGTTACC G^GT[ACGT]ACC
BstPI 6049 GGTTACC G^GT[ACGT]ACC
Bsu36I 4235 CCTCAGG CC^T[ACGT]AGG
DrdI 6027 GACCGCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 74 CGGCCG C^GGCCG
Eam1105I 1172 GACCCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 74 CGGCCG C^GGCCG
Eco52I 74 CGGCCG C^GGCCG
Eco81I 4235 CCTCAGG CC^T[ACGT]AGG
EcoO65I 6049 GGTTACC G^GT[ACGT]ACC
EcoRI 1828 GAATTC G^AATTC
EcoRV 4084 GATATC GAT^ATC
FseI 75 GGCCGGCC GGCCGG^CC
NaeI 76 GCCGGC GCC^GGC
NgoMIV 76 GCCGGC G^CCGGC
PaeR7I 3690 CTCGAG C^TCGAG
PflFI 4762 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 927 ACCGGT A^CCGGT
PpuMI 68 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 1427 ATTAAT AT^TAAT
Psp1406I 1380 AACGTT AA^CGTT
ScaI 2895 AGTACT AGT^ACT
Tth111I 4762 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3690 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 405 GTCTAC GT^[AC][GT]AC
818 GTAGAC
BbvCI 295 CCTCAGC CCTCAGC(-5/-2)
5058 CCTCAGC
BciVI 3155 GGATAC GTATCC(6/5)
5731 GTATCC
BsiEI 74 CGGCCG CG[GA][CT]^CG
116 CGACCG
BspDI 138 ATCGAT AT^CGAT
5999 ATCGAT
BssSI 740 CACGAG CACGAG(-5/-1)
3094 CACGAG
BstBI 2371 TTCGAA TT^CGAA
3962 TTCGAA
ClaI 138 ATCGAT AT^CGAT
5999 ATCGAT
DraIII 725 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1077 CACCTGGTG
FauI 107 CCCGC CCCGC(4/6)
6447 CCCGC
HaeII 423 AGCGCC [GA]GCGC^[CT]
2669 AGCGCC
MfeI 5403 CAATTG C^AATTG
6204 CAATTG
MunI 5403 CAATTG C^AATTG
6204 CAATTG
NspV 2371 TTCGAA TT^CGAA
3962 TTCGAA
PflMI 1757 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5068 CCAAGTGCTGG
SexAI 1078 ACCTGGT A^CC[AT]GGT
6494 ACCTGGT
SfuI 2371 TTCGAA TT^CGAA
3962 TTCGAA
SspI 5253 AATATT AAT^ATT
6614 AATATT
Van91I 1757 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5068 CCAAGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbrPI
BbsI BbvI BclI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I EcoT22I FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeI NdeII NheI NlaIII NlaIV
NsiI NspI PleI PmaCI PmlI
PstI PvuII RcaI RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII