################################## # NO CUT ENZYMES ( 42 enzymes) # ################################## AatII AccIII AgeI ApaI BbrPI BbvCI BcgI BclI BsaAI BseAI BsiWI BspDI BspEI BsrBI Bst1107I BstBI BstZ17I ClaI EcoRV FbaI FseI MfeI MluI MroI MunI NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI PvuI SacI SbfI SfuI SgrAI SnaBI Sse8387I SwaI XbaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### Acc65I 1707 GGTACC G^GTACC AccI 952 GTCGAC GT^[AC][GT]AC AclI 2448 AACGTT AA^CGTT AscI 1059 GGCGCGCC GG^CGCGCC AseI 4505 ATTAAT AT^TAAT AsnI 4505 ATTAAT AT^TAAT Asp700I 3590 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1707 GGTACC G^GTACC AviII 680 TGCGCA TGC^GCA BciVI 2366 GTATCC GTATCC(6/5) BglII 4734 AGATCT A^GATCT BsaBI 3643 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 249 GAGACG CGTCTC(1/5) BspLU11I 3623 ACATGT A^CATGT BstAPI 4079 GCATTTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CpoI 1879 CGGACCG CG^G[AT]CCG DraIII 2454 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3654 GACAGACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 3563 GAATTC G^AATTC FspI 680 TGCGCA TGC^GCA HpaI 4552 GTTAAC GTT^AAC KpnI 1707 GGTACC GGTAC^C MamI 3643 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NdeI 5752 CATATG CA^TATG NotI 1208 GCGGCCGC GC^GGCCGC PaeR7I 372 CTCGAG C^TCGAG PshBI 4505 ATTAAT AT^TAAT Psp1406I 2448 AACGTT AA^CGTT RsrII 1879 CGGACCG CG^G[AT]CCG SalI 952 GTCGAC G^TCGAC SfiI 488 GGCCGTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspI 5446 AATATT AAT^ATT StuI 1673 AGGCCT AGG^CCT XhoI 372 CTCGAG C^TCGAG XmnI 3590 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AflIII 3623 ACATGT A^C[GA][CT]GT 5531 ACACGT Alw44I 429 GTGCAC G^TGCAC 5277 GTGCAC ApaLI 429 GTGCAC G^TGCAC 5277 GTGCAC AspI 444 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4455 GACCCAGTC BamHI 332 GGATCC G^GATCC 1818 GGATCC BsaWI 852 TCCGGT [AT]^CCGG[AT] 2580 ACCGGA BsmI 2320 GAATGC GAATGC(1/-1) 3137 GCATTC BspHI 796 TCATGA T^CATGA 3742 TCATGA BssSI 348 CACGAG CACGAG(-5/-1) 1371 CACGAG BstEII 1184 GGTCACC G^GT[ACGT]ACC 3381 GGTTACC BstPI 1184 GGTCACC G^GT[ACGT]ACC 3381 GGTTACC DraI 2192 TTTAAA TTT^AAA 2410 TTTAAA EcoNI 4024 CCTAGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5781 CCTGTTTGAGG EcoO65I 1184 GGTCACC G^GT[ACGT]ACC 3381 GGTTACC EcoT22I 3892 ATGCAT ATGCA^T 4086 ATGCAT KspI 193 CCGCGG CCGC^GG 1883 CCGCGG NaeI 648 GCCGGC GCC^GGC 5494 GCCGGC NgoMIV 648 GCCGGC G^CCGGC 5494 GCCGGC NheI 1973 GCTAGC G^CTAGC 3102 GCTAGC NsiI 3892 ATGCAT ATGCA^T 4086 ATGCAT PflFI 444 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4455 GACCCAGTC RcaI 796 TCATGA T^CATGA 3742 TCATGA SacII 193 CCGCGG CCGC^GG 1883 CCGCGG SapI 312 GAAGAGC GCTCTTC(1/4) 1091 GCTCTTC ScaI 1200 AGTACT AGT^ACT 2508 AGTACT SmaI 414 CCCGGG CCC^GGG 532 CCCGGG SpeI 5726 ACTAGT A^CTAGT 5743 ACTAGT Tth111I 444 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4455 GACCCAGTC XmaCI 414 CCCGGG C^CCGGG 532 CCCGGG XmaI 414 CCCGGG C^CCGGG 532 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 151 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflII AhdI AluI AlwI AlwNI AocI Aor51HI ApoI AspEI AspHI AvaI AvaII AvrII BalI BanI BanII BbeI BbsI BbvI BcnI BfaI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrFI BsrGI BsrI BssHII BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SfoI SmlI SphI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII