##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
AatII AccIII AgeI ApaI BbrPI
BbvCI BcgI BclI BsaAI BseAI
BsiWI BspDI BspEI BsrBI Bst1107I
BstBI BstZ17I ClaI EcoRV FbaI
FseI MfeI MluI MroI MunI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PvuI SacI
SbfI SfuI SgrAI SnaBI Sse8387I
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 1707 GGTACC G^GTACC
AccI 952 GTCGAC GT^[AC][GT]AC
AclI 2448 AACGTT AA^CGTT
AscI 1059 GGCGCGCC GG^CGCGCC
AseI 4505 ATTAAT AT^TAAT
AsnI 4505 ATTAAT AT^TAAT
Asp700I 3590 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1707 GGTACC G^GTACC
AviII 680 TGCGCA TGC^GCA
BciVI 2366 GTATCC GTATCC(6/5)
BglII 4734 AGATCT A^GATCT
BsaBI 3643 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 249 GAGACG CGTCTC(1/5)
BspLU11I 3623 ACATGT A^CATGT
BstAPI 4079 GCATTTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 1879 CGGACCG CG^G[AT]CCG
DraIII 2454 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3654 GACAGACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3563 GAATTC G^AATTC
FspI 680 TGCGCA TGC^GCA
HpaI 4552 GTTAAC GTT^AAC
KpnI 1707 GGTACC GGTAC^C
MamI 3643 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 5752 CATATG CA^TATG
NotI 1208 GCGGCCGC GC^GGCCGC
PaeR7I 372 CTCGAG C^TCGAG
PshBI 4505 ATTAAT AT^TAAT
Psp1406I 2448 AACGTT AA^CGTT
RsrII 1879 CGGACCG CG^G[AT]CCG
SalI 952 GTCGAC G^TCGAC
SfiI 488 GGCCGTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 5446 AATATT AAT^ATT
StuI 1673 AGGCCT AGG^CCT
XhoI 372 CTCGAG C^TCGAG
XmnI 3590 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 3623 ACATGT A^C[GA][CT]GT
5531 ACACGT
Alw44I 429 GTGCAC G^TGCAC
5277 GTGCAC
ApaLI 429 GTGCAC G^TGCAC
5277 GTGCAC
AspI 444 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4455 GACCCAGTC
BamHI 332 GGATCC G^GATCC
1818 GGATCC
BsaWI 852 TCCGGT [AT]^CCGG[AT]
2580 ACCGGA
BsmI 2320 GAATGC GAATGC(1/-1)
3137 GCATTC
BspHI 796 TCATGA T^CATGA
3742 TCATGA
BssSI 348 CACGAG CACGAG(-5/-1)
1371 CACGAG
BstEII 1184 GGTCACC G^GT[ACGT]ACC
3381 GGTTACC
BstPI 1184 GGTCACC G^GT[ACGT]ACC
3381 GGTTACC
DraI 2192 TTTAAA TTT^AAA
2410 TTTAAA
EcoNI 4024 CCTAGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5781 CCTGTTTGAGG
EcoO65I 1184 GGTCACC G^GT[ACGT]ACC
3381 GGTTACC
EcoT22I 3892 ATGCAT ATGCA^T
4086 ATGCAT
KspI 193 CCGCGG CCGC^GG
1883 CCGCGG
NaeI 648 GCCGGC GCC^GGC
5494 GCCGGC
NgoMIV 648 GCCGGC G^CCGGC
5494 GCCGGC
NheI 1973 GCTAGC G^CTAGC
3102 GCTAGC
NsiI 3892 ATGCAT ATGCA^T
4086 ATGCAT
PflFI 444 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4455 GACCCAGTC
RcaI 796 TCATGA T^CATGA
3742 TCATGA
SacII 193 CCGCGG CCGC^GG
1883 CCGCGG
SapI 312 GAAGAGC GCTCTTC(1/4)
1091 GCTCTTC
ScaI 1200 AGTACT AGT^ACT
2508 AGTACT
SmaI 414 CCCGGG CCC^GGG
532 CCCGGG
SpeI 5726 ACTAGT A^CTAGT
5743 ACTAGT
Tth111I 444 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4455 GACCCAGTC
XmaCI 414 CCCGGG C^CCGGG
532 CCCGGG
XmaI 414 CCCGGG C^CCGGG
532 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 151 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AhdI AluI AlwI AlwNI
AocI Aor51HI ApoI AspEI AspHI
AvaI AvaII AvrII BalI BanI
BanII BbeI BbsI BbvI BcnI
BfaI BfrI BglI BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrDI
BsrFI BsrGI BsrI BssHII BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI SmlI SphI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII