################################## # NO CUT ENZYMES ( 45 enzymes) # ################################## AatII AclI AflII AgeI Alw44I ApaI ApaLI AscI AseI AsnI AviII BfrI BsiWI BsrBI BssHII Bst1107I BstZ17I CpoI DraIII DrdI FseI FspI HpaI KspI MluI NheI NotI NruI PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SgrAI SnaBI SpeI Sse8387I XhoI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### Acc65I 3083 GGTACC G^GTACC Asp718I 3083 GGTACC G^GTACC AspI 1410 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 2950 CCTAGG C^CTAGG BamHI 4487 GGATCC G^GATCC BcgI 5656 GCATTTGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 4516 GTATCC GTATCC(6/5) BlnI 2950 CCTAGG C^CTAGG BspDI 5195 ATCGAT AT^CGAT BspHI 22 TCATGA T^CATGA BspLU11I 900 ACATGT A^CATGT BstEII 5971 GGTGACC G^GT[ACGT]ACC BstPI 5971 GGTGACC G^GT[ACGT]ACC ClaI 5195 ATCGAT AT^CGAT EagI 6044 CGGCCG C^GGCCG EclXI 6044 CGGCCG C^GGCCG Eco52I 6044 CGGCCG C^GGCCG EcoO65I 5971 GGTGACC G^GT[ACGT]ACC HindIII 2567 AAGCTT A^AGCTT KpnI 3083 GGTACC GGTAC^C NdeI 3659 CATATG CA^TATG PflFI 1410 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 22 TCATGA T^CATGA SexAI 2025 ACCTGGT A^CC[AT]GGT SmaI 4471 CCCGGG CCC^GGG SphI 3697 GCATGC GCATG^C StuI 2777 AGGCCT AGG^CCT SwaI 5847 ATTTAAAT ATTT^AAAT Tth111I 1410 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 3247 TCTAGA T^CTAGA XmaCI 4471 CCCGGG C^CCGGG XmaI 4471 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### AccI 1440 GTAGAC GT^[AC][GT]AC 2819 GTAGAC AcyI 597 GGCGCC G[GA]^CG[CT]C 612 GGCGCC AhdI 1952 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4396 GACTTCCTGTC Aor51HI 2010 AGCGCT AGC^GCT 3207 AGCGCT Asp700I 2273 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5045 GAAGGAATTC AspEI 1952 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4396 GACTTCCTGTC BbeI 597 GGCGCC GGCGC^C 612 GGCGCC BbrPI 2629 CACGTG CAC^GTG 3176 CACGTG BclI 40 TGATCA T^GATCA 4186 TGATCA BsaHI 597 GGCGCC G[GA]^CG[CT]C 612 GGCGCC BsiEI 5344 CGACCG CG[GA][CT]^CG 6044 CGGCCG BsmI 1693 GAATGC GAATGC(1/-1) 4144 GAATGC BsrDI 2091 CATTGC GCAATG(2/0) 3039 GCAATG BssSI 3884 CACGAG CACGAG(-5/-1) 6194 CACGAG BstBI 917 TTCGAA TT^CGAA 2270 TTCGAA DraI 3707 TTTAAA TTT^AAA 5848 TTTAAA Eam1105I 1952 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4396 GACTTCCTGTC Eco47III 2010 AGCGCT AGC^GCT 3207 AGCGCT EcoRV 1673 GATATC GAT^ATC 4696 GATATC EcoT22I 819 ATGCAT ATGCA^T 5509 ATGCAT FbaI 40 TGATCA T^GATCA 4186 TGATCA Hin1I 597 GGCGCC G[GA]^CG[CT]C 612 GGCGCC HincII 1042 GTTGAC GT[CT]^[GA]AC 3437 GTTGAC HindII 1042 GTTGAC GT[CT]^[GA]AC 3437 GTTGAC KasI 597 GGCGCC G^GCGCC 612 GGCGCC MfeI 1667 CAATTG C^AATTG 2361 CAATTG MunI 1667 CAATTG C^AATTG 2361 CAATTG NaeI 615 GCCGGC GCC^GGC 4674 GCCGGC NarI 597 GGCGCC GG^CGCC 612 GGCGCC NgoMIV 615 GCCGGC G^CCGGC 4674 GCCGGC NsiI 819 ATGCAT ATGCA^T 5509 ATGCAT NspV 917 TTCGAA TT^CGAA 2270 TTCGAA PflMI 3484 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4972 CCAGATTGTGG PmaCI 2629 CACGTG CAC^GTG 3176 CACGTG PmlI 2629 CACGTG CAC^GTG 3176 CACGTG SfoI 597 GGCGCC GGC^GCC 612 GGCGCC SfuI 917 TTCGAA TT^CGAA 2270 TTCGAA SspI 4367 AATATT AAT^ATT 5443 AATATT Van91I 3484 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4972 CCAGATTGTGG XmnI 2273 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5045 GAAGGAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 141 enzymes) # ######################################### AccII AccIII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspEI BspMI BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PshAI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII