##################################
# NO CUT ENZYMES (   45 enzymes) #
##################################

AatII       AclI        AflII       AgeI        Alw44I      
ApaI        ApaLI       AscI        AseI        AsnI        
AviII       BfrI        BsiWI       BsrBI       BssHII      
Bst1107I    BstZ17I     CpoI        DraIII      DrdI        
FseI        FspI        HpaI        KspI        MluI        
NheI        NotI        NruI        PacI        PaeR7I      
PinAI       PmeI        PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        SfiI        
SgrAI       SnaBI       SpeI        Sse8387I    XhoI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

Acc65I       3083  GGTACC               G^GTACC
Asp718I      3083  GGTACC               G^GTACC
AspI         1410  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        2950  CCTAGG               C^CTAGG
BamHI        4487  GGATCC               G^GATCC
BcgI         5656  GCATTTGGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        4516  GTATCC               GTATCC(6/5)
BlnI         2950  CCTAGG               C^CTAGG
BspDI        5195  ATCGAT               AT^CGAT
BspHI          22  TCATGA               T^CATGA
BspLU11I      900  ACATGT               A^CATGT
BstEII       5971  GGTGACC              G^GT[ACGT]ACC
BstPI        5971  GGTGACC              G^GT[ACGT]ACC
ClaI         5195  ATCGAT               AT^CGAT
EagI         6044  CGGCCG               C^GGCCG
EclXI        6044  CGGCCG               C^GGCCG
Eco52I       6044  CGGCCG               C^GGCCG
EcoO65I      5971  GGTGACC              G^GT[ACGT]ACC
HindIII      2567  AAGCTT               A^AGCTT
KpnI         3083  GGTACC               GGTAC^C
NdeI         3659  CATATG               CA^TATG
PflFI        1410  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI           22  TCATGA               T^CATGA
SexAI        2025  ACCTGGT              A^CC[AT]GGT
SmaI         4471  CCCGGG               CCC^GGG
SphI         3697  GCATGC               GCATG^C
StuI         2777  AGGCCT               AGG^CCT
SwaI         5847  ATTTAAAT             ATTT^AAAT
Tth111I      1410  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         3247  TCTAGA               T^CTAGA
XmaCI        4471  CCCGGG               C^CCGGG
XmaI         4471  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   40 enzymes) #
###################################

AccI         1440  GTAGAC               GT^[AC][GT]AC
             2819  GTAGAC              
AcyI          597  GGCGCC               G[GA]^CG[CT]C
              612  GGCGCC              
AhdI         1952  GACCCTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4396  GACTTCCTGTC         
Aor51HI      2010  AGCGCT               AGC^GCT
             3207  AGCGCT              
Asp700I      2273  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5045  GAAGGAATTC          
AspEI        1952  GACCCTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4396  GACTTCCTGTC         
BbeI          597  GGCGCC               GGCGC^C
              612  GGCGCC              
BbrPI        2629  CACGTG               CAC^GTG
             3176  CACGTG              
BclI           40  TGATCA               T^GATCA
             4186  TGATCA              
BsaHI         597  GGCGCC               G[GA]^CG[CT]C
              612  GGCGCC              
BsiEI        5344  CGACCG               CG[GA][CT]^CG
             6044  CGGCCG              
BsmI         1693  GAATGC               GAATGC(1/-1)
             4144  GAATGC              
BsrDI        2091  CATTGC               GCAATG(2/0)
             3039  GCAATG              
BssSI        3884  CACGAG               CACGAG(-5/-1)
             6194  CACGAG              
BstBI         917  TTCGAA               TT^CGAA
             2270  TTCGAA              
DraI         3707  TTTAAA               TTT^AAA
             5848  TTTAAA              
Eam1105I     1952  GACCCTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4396  GACTTCCTGTC         
Eco47III     2010  AGCGCT               AGC^GCT
             3207  AGCGCT              
EcoRV        1673  GATATC               GAT^ATC
             4696  GATATC              
EcoT22I       819  ATGCAT               ATGCA^T
             5509  ATGCAT              
FbaI           40  TGATCA               T^GATCA
             4186  TGATCA              
Hin1I         597  GGCGCC               G[GA]^CG[CT]C
              612  GGCGCC              
HincII       1042  GTTGAC               GT[CT]^[GA]AC
             3437  GTTGAC              
HindII       1042  GTTGAC               GT[CT]^[GA]AC
             3437  GTTGAC              
KasI          597  GGCGCC               G^GCGCC
              612  GGCGCC              
MfeI         1667  CAATTG               C^AATTG
             2361  CAATTG              
MunI         1667  CAATTG               C^AATTG
             2361  CAATTG              
NaeI          615  GCCGGC               GCC^GGC
             4674  GCCGGC              
NarI          597  GGCGCC               GG^CGCC
              612  GGCGCC              
NgoMIV        615  GCCGGC               G^CCGGC
             4674  GCCGGC              
NsiI          819  ATGCAT               ATGCA^T
             5509  ATGCAT              
NspV          917  TTCGAA               TT^CGAA
             2270  TTCGAA              
PflMI        3484  CCACCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4972  CCAGATTGTGG         
PmaCI        2629  CACGTG               CAC^GTG
             3176  CACGTG              
PmlI         2629  CACGTG               CAC^GTG
             3176  CACGTG              
SfoI          597  GGCGCC               GGC^GCC
              612  GGCGCC              
SfuI          917  TTCGAA               TT^CGAA
             2270  TTCGAA              
SspI         4367  AATATT               AAT^ATT
             5443  AATATT              
Van91I       3484  CCACCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4972  CCAGATTGTGG         
XmnI         2273  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5045  GAAGGAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  141 enzymes) #
#########################################

AccII       AccIII      AciI        AcsI        AfaI        
AflIII      AluI        AlwI        AlwNI       AocI        
ApoI        AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvCI       BbvI        
BcnI        BfaI        BglI        BglII       BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
Bsp1407I    BspEI       BspMI       BsrFI       BsrGI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoRI       EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MamI        MboI        
MboII       MflI        MluNI       MnlI        MroI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PshAI       PstI        PvuII       RsaI        
SacI        SapI        Sau3AI      Sau96I      ScaI        
ScrFI       SfaNI       SfcI        SmlI        SspBI       
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII