##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
ApaI ApaLI AscI AseI AsnI
AviII BfrI BsiWI BsrBI BssHII
Bst1107I BstZ17I CpoI DraIII DrdI
FseI FspI HpaI KspI MluI
NheI NotI NruI PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SgrAI SnaBI SpeI Sse8387I XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 3083 GGTACC G^GTACC
Asp718I 3083 GGTACC G^GTACC
AspI 1410 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2950 CCTAGG C^CTAGG
BamHI 4487 GGATCC G^GATCC
BcgI 5656 GCATTTGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4516 GTATCC GTATCC(6/5)
BlnI 2950 CCTAGG C^CTAGG
BspDI 5195 ATCGAT AT^CGAT
BspHI 22 TCATGA T^CATGA
BspLU11I 900 ACATGT A^CATGT
BstEII 5971 GGTGACC G^GT[ACGT]ACC
BstPI 5971 GGTGACC G^GT[ACGT]ACC
ClaI 5195 ATCGAT AT^CGAT
EagI 6044 CGGCCG C^GGCCG
EclXI 6044 CGGCCG C^GGCCG
Eco52I 6044 CGGCCG C^GGCCG
EcoO65I 5971 GGTGACC G^GT[ACGT]ACC
HindIII 2567 AAGCTT A^AGCTT
KpnI 3083 GGTACC GGTAC^C
NdeI 3659 CATATG CA^TATG
PflFI 1410 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 22 TCATGA T^CATGA
SexAI 2025 ACCTGGT A^CC[AT]GGT
SmaI 4471 CCCGGG CCC^GGG
SphI 3697 GCATGC GCATG^C
StuI 2777 AGGCCT AGG^CCT
SwaI 5847 ATTTAAAT ATTT^AAAT
Tth111I 1410 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3247 TCTAGA T^CTAGA
XmaCI 4471 CCCGGG C^CCGGG
XmaI 4471 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccI 1440 GTAGAC GT^[AC][GT]AC
2819 GTAGAC
AcyI 597 GGCGCC G[GA]^CG[CT]C
612 GGCGCC
AhdI 1952 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4396 GACTTCCTGTC
Aor51HI 2010 AGCGCT AGC^GCT
3207 AGCGCT
Asp700I 2273 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5045 GAAGGAATTC
AspEI 1952 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4396 GACTTCCTGTC
BbeI 597 GGCGCC GGCGC^C
612 GGCGCC
BbrPI 2629 CACGTG CAC^GTG
3176 CACGTG
BclI 40 TGATCA T^GATCA
4186 TGATCA
BsaHI 597 GGCGCC G[GA]^CG[CT]C
612 GGCGCC
BsiEI 5344 CGACCG CG[GA][CT]^CG
6044 CGGCCG
BsmI 1693 GAATGC GAATGC(1/-1)
4144 GAATGC
BsrDI 2091 CATTGC GCAATG(2/0)
3039 GCAATG
BssSI 3884 CACGAG CACGAG(-5/-1)
6194 CACGAG
BstBI 917 TTCGAA TT^CGAA
2270 TTCGAA
DraI 3707 TTTAAA TTT^AAA
5848 TTTAAA
Eam1105I 1952 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4396 GACTTCCTGTC
Eco47III 2010 AGCGCT AGC^GCT
3207 AGCGCT
EcoRV 1673 GATATC GAT^ATC
4696 GATATC
EcoT22I 819 ATGCAT ATGCA^T
5509 ATGCAT
FbaI 40 TGATCA T^GATCA
4186 TGATCA
Hin1I 597 GGCGCC G[GA]^CG[CT]C
612 GGCGCC
HincII 1042 GTTGAC GT[CT]^[GA]AC
3437 GTTGAC
HindII 1042 GTTGAC GT[CT]^[GA]AC
3437 GTTGAC
KasI 597 GGCGCC G^GCGCC
612 GGCGCC
MfeI 1667 CAATTG C^AATTG
2361 CAATTG
MunI 1667 CAATTG C^AATTG
2361 CAATTG
NaeI 615 GCCGGC GCC^GGC
4674 GCCGGC
NarI 597 GGCGCC GG^CGCC
612 GGCGCC
NgoMIV 615 GCCGGC G^CCGGC
4674 GCCGGC
NsiI 819 ATGCAT ATGCA^T
5509 ATGCAT
NspV 917 TTCGAA TT^CGAA
2270 TTCGAA
PflMI 3484 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4972 CCAGATTGTGG
PmaCI 2629 CACGTG CAC^GTG
3176 CACGTG
PmlI 2629 CACGTG CAC^GTG
3176 CACGTG
SfoI 597 GGCGCC GGC^GCC
612 GGCGCC
SfuI 917 TTCGAA TT^CGAA
2270 TTCGAA
SspI 4367 AATATT AAT^ATT
5443 AATATT
Van91I 3484 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4972 CCAGATTGTGG
XmnI 2273 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5045 GAAGGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AccIII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspEI BspMI BsrFI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PshAI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII