##################################
# NO CUT ENZYMES (   52 enzymes) #
##################################

AatII       AclI        AflII       AgeI        AhdI        
AscI        AseI        AsnI        AspEI       BciVI       
BfrI        BsiEI       BsiWI       BspDI       BsrDI       
BssHII      Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        EagI        Eam1105I    EclXI       Eco52I      
EcoRI       FseI        HpaI        KspI        MfeI        
MluI        MunI        NcoI        NheI        NotI        
NruI        NspV        PacI        PinAI       PmeI        
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        SfuI        SnaBI       SpeI        
Sse8387I    SwaI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AflIII       4689  ACATGT               A^C[GA][CT]GT
Aor51HI      4159  AGCGCT               AGC^GCT
AspI         1401  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        2254  TGCGCA               TGC^GCA
AvrII        2955  CCTAGG               C^CTAGG
BbrPI        5222  CACGTG               CAC^GTG
BbvCI        1271  CCTCAGC              CCTCAGC(-5/-2)
BcgI          326  CGATGAGGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         2752  TGATCA               T^GATCA
BglII        3728  AGATCT               A^GATCT
BlnI         2955  CCTAGG               C^CTAGG
BsaAI        5222  CACGTG               [CT]AC^GT[GA]
BsaBI        2753  GATCAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        4783  GAGACG               CGTCTC(1/5)
Bsp1407I     5363  TGTACA               T^GTACA
BspLU11I     4689  ACATGT               A^CATGT
BsrGI        5363  TGTACA               T^GTACA
BstXI        4470  CCACCCCCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI         5076  CCGTGG               C^C[GA][CT]GG
Eco47III     4159  AGCGCT               AGC^GCT
FbaI         2752  TGATCA               T^GATCA
FspI         2254  TGCGCA               TGC^GCA
HindIII      4396  AAGCTT               A^AGCTT
MamI         2753  GATCAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI         3153  CATATG               CA^TATG
PaeR7I       1008  CTCGAG               C^TCGAG
PflFI        1401  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        5222  CACGTG               CAC^GTG
PmlI         5222  CACGTG               CAC^GTG
PshAI        4413  GACAGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI        4660  ACCTGGT              A^CC[AT]GGT
SspBI        5363  TGTACA               T^GTACA
SspI          463  AATATT               AAT^ATT
Tth111I      1401  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         1008  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

Alw44I        372  GTGCAC               G^TGCAC
             5396  GTGCAC              
ApaLI         372  GTGCAC               G^TGCAC
             5396  GTGCAC              
Asp700I      2777  GAACCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3609  GAAGCTCTTC          
BalI          541  TGGCCA               TGG^CCA
             1474  TGGCCA              
BamHI        2430  GGATCC               G^GATCC
             2945  GGATCC              
BlpI         1418  GCTCAGC              GC^T[ACGT]AGC
             3076  GCTAAGC             
Bpu1102I     1418  GCTCAGC              GC^T[ACGT]AGC
             3076  GCTAAGC             
BspHI         421  TCATGA               T^CATGA
             4017  TCATGA              
BstAPI         38  GCAGGCCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3149  GCATCATATGC         
BstEII       3349  GGTCACC              G^GT[ACGT]ACC
             5636  GGTTACC             
BstPI        3349  GGTCACC              G^GT[ACGT]ACC
             5636  GGTTACC             
CelII        1418  GCTCAGC              GC^T[ACGT]AGC
             3076  GCTAAGC             
DrdI         1160  GACAGTCCGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2562  GACAGCGTTGTC        
EcoO65I      3349  GGTCACC              G^GT[ACGT]ACC
             5636  GGTTACC             
EcoRV        1833  GATATC               GAT^ATC
             2292  GATATC              
EcoT22I      3147  ATGCAT               ATGCA^T
             4752  ATGCAT              
HincII       2076  GTTGAC               GT[CT]^[GA]AC
             5291  GTTGAC              
HindII       2076  GTTGAC               GT[CT]^[GA]AC
             5291  GTTGAC              
MaeII        2232  ACGT                 A^CGT
             5223  ACGT                
MluNI         541  TGGCCA               TGG^CCA
             1474  TGGCCA              
MscI          541  TGGCCA               TGG^CCA
             1474  TGGCCA              
NaeI           31  GCCGGC               GCC^GGC
             4486  GCCGGC              
NgoMIV         31  GCCGGC               G^CCGGC
             4486  GCCGGC              
NsiI         3147  ATGCAT               ATGCA^T
             4752  ATGCAT              
RcaI          421  TCATGA               T^CATGA
             4017  TCATGA              
ScaI         1630  AGTACT               AGT^ACT
             5612  AGTACT              
SfiI         1269  GGCCTCAGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             3891  GGCCACTATGGCC       
SgrAI          30  CGCCGGCG             C[GA]^CCGG[CT]G
              380  CGCCGGTG            
SmaI          669  CCCGGG               CCC^GGG
             1221  CCCGGG              
StuI         2309  AGGCCT               AGG^CCT
             4378  AGGCCT              
XcmI          529  CCAAGGGCCTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1462  CCAGCTCTCCTTTGG     
XmaCI         669  CCCGGG               C^CCGGG
             1221  CCCGGG              
XmaI          669  CCCGGG               C^CCGGG
             1221  CCCGGG              
XmnI         2777  GAACCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3609  GAAGCTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  137 enzymes) #
#########################################

Acc65I      AccI        AccII       AccIII      AciI        
AcsI        AcyI        AfaI        AluI        AlwI        
AlwNI       AocI        ApaI        ApoI        Asp718I     
AspHI       AvaI        AvaII       BanI        BanII       
BbeI        BbsI        BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BpuAI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspEI       
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BssSI       BstF5I      BstNI       BstUI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraI        DraII       DraIII      
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        KpnI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MroI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SfoI        SmlI        SphI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XbaI        XhoII