##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
AscI AseI AsnI AspEI BciVI
BfrI BsiEI BsiWI BspDI BsrDI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco52I
EcoRI FseI HpaI KspI MfeI
MluI MunI NcoI NheI NotI
NruI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SfuI SnaBI SpeI
Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 4689 ACATGT A^C[GA][CT]GT
Aor51HI 4159 AGCGCT AGC^GCT
AspI 1401 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2254 TGCGCA TGC^GCA
AvrII 2955 CCTAGG C^CTAGG
BbrPI 5222 CACGTG CAC^GTG
BbvCI 1271 CCTCAGC CCTCAGC(-5/-2)
BcgI 326 CGATGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2752 TGATCA T^GATCA
BglII 3728 AGATCT A^GATCT
BlnI 2955 CCTAGG C^CTAGG
BsaAI 5222 CACGTG [CT]AC^GT[GA]
BsaBI 2753 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 4783 GAGACG CGTCTC(1/5)
Bsp1407I 5363 TGTACA T^GTACA
BspLU11I 4689 ACATGT A^CATGT
BsrGI 5363 TGTACA T^GTACA
BstXI 4470 CCACCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 5076 CCGTGG C^C[GA][CT]GG
Eco47III 4159 AGCGCT AGC^GCT
FbaI 2752 TGATCA T^GATCA
FspI 2254 TGCGCA TGC^GCA
HindIII 4396 AAGCTT A^AGCTT
MamI 2753 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 3153 CATATG CA^TATG
PaeR7I 1008 CTCGAG C^TCGAG
PflFI 1401 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 5222 CACGTG CAC^GTG
PmlI 5222 CACGTG CAC^GTG
PshAI 4413 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 4660 ACCTGGT A^CC[AT]GGT
SspBI 5363 TGTACA T^GTACA
SspI 463 AATATT AAT^ATT
Tth111I 1401 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1008 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Alw44I 372 GTGCAC G^TGCAC
5396 GTGCAC
ApaLI 372 GTGCAC G^TGCAC
5396 GTGCAC
Asp700I 2777 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3609 GAAGCTCTTC
BalI 541 TGGCCA TGG^CCA
1474 TGGCCA
BamHI 2430 GGATCC G^GATCC
2945 GGATCC
BlpI 1418 GCTCAGC GC^T[ACGT]AGC
3076 GCTAAGC
Bpu1102I 1418 GCTCAGC GC^T[ACGT]AGC
3076 GCTAAGC
BspHI 421 TCATGA T^CATGA
4017 TCATGA
BstAPI 38 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3149 GCATCATATGC
BstEII 3349 GGTCACC G^GT[ACGT]ACC
5636 GGTTACC
BstPI 3349 GGTCACC G^GT[ACGT]ACC
5636 GGTTACC
CelII 1418 GCTCAGC GC^T[ACGT]AGC
3076 GCTAAGC
DrdI 1160 GACAGTCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2562 GACAGCGTTGTC
EcoO65I 3349 GGTCACC G^GT[ACGT]ACC
5636 GGTTACC
EcoRV 1833 GATATC GAT^ATC
2292 GATATC
EcoT22I 3147 ATGCAT ATGCA^T
4752 ATGCAT
HincII 2076 GTTGAC GT[CT]^[GA]AC
5291 GTTGAC
HindII 2076 GTTGAC GT[CT]^[GA]AC
5291 GTTGAC
MaeII 2232 ACGT A^CGT
5223 ACGT
MluNI 541 TGGCCA TGG^CCA
1474 TGGCCA
MscI 541 TGGCCA TGG^CCA
1474 TGGCCA
NaeI 31 GCCGGC GCC^GGC
4486 GCCGGC
NgoMIV 31 GCCGGC G^CCGGC
4486 GCCGGC
NsiI 3147 ATGCAT ATGCA^T
4752 ATGCAT
RcaI 421 TCATGA T^CATGA
4017 TCATGA
ScaI 1630 AGTACT AGT^ACT
5612 AGTACT
SfiI 1269 GGCCTCAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3891 GGCCACTATGGCC
SgrAI 30 CGCCGGCG C[GA]^CCGG[CT]G
380 CGCCGGTG
SmaI 669 CCCGGG CCC^GGG
1221 CCCGGG
StuI 2309 AGGCCT AGG^CCT
4378 AGGCCT
XcmI 529 CCAAGGGCCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1462 CCAGCTCTCCTTTGG
XmaCI 669 CCCGGG C^CCGGG
1221 CCCGGG
XmaI 669 CCCGGG C^CCGGG
1221 CCCGGG
XmnI 2777 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3609 GAAGCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
Acc65I AccI AccII AccIII AciI
AcsI AcyI AfaI AluI AlwI
AlwNI AocI ApaI ApoI Asp718I
AspHI AvaI AvaII BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspEI
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI KpnI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MroI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XhoII