################################## # NO CUT ENZYMES ( 52 enzymes) # ################################## AatII AclI AflII AgeI AhdI AscI AseI AsnI AspEI BciVI BfrI BsiEI BsiWI BspDI BsrDI BssHII Bst1107I BstBI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco52I EcoRI FseI HpaI KspI MfeI MluI MunI NcoI NheI NotI NruI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfuI SnaBI SpeI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AflIII 4689 ACATGT A^C[GA][CT]GT Aor51HI 4159 AGCGCT AGC^GCT AspI 1401 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2254 TGCGCA TGC^GCA AvrII 2955 CCTAGG C^CTAGG BbrPI 5222 CACGTG CAC^GTG BbvCI 1271 CCTCAGC CCTCAGC(-5/-2) BcgI 326 CGATGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2752 TGATCA T^GATCA BglII 3728 AGATCT A^GATCT BlnI 2955 CCTAGG C^CTAGG BsaAI 5222 CACGTG [CT]AC^GT[GA] BsaBI 2753 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 4783 GAGACG CGTCTC(1/5) Bsp1407I 5363 TGTACA T^GTACA BspLU11I 4689 ACATGT A^CATGT BsrGI 5363 TGTACA T^GTACA BstXI 4470 CCACCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 5076 CCGTGG C^C[GA][CT]GG Eco47III 4159 AGCGCT AGC^GCT FbaI 2752 TGATCA T^GATCA FspI 2254 TGCGCA TGC^GCA HindIII 4396 AAGCTT A^AGCTT MamI 2753 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NdeI 3153 CATATG CA^TATG PaeR7I 1008 CTCGAG C^TCGAG PflFI 1401 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 5222 CACGTG CAC^GTG PmlI 5222 CACGTG CAC^GTG PshAI 4413 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SexAI 4660 ACCTGGT A^CC[AT]GGT SspBI 5363 TGTACA T^GTACA SspI 463 AATATT AAT^ATT Tth111I 1401 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1008 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### Alw44I 372 GTGCAC G^TGCAC 5396 GTGCAC ApaLI 372 GTGCAC G^TGCAC 5396 GTGCAC Asp700I 2777 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3609 GAAGCTCTTC BalI 541 TGGCCA TGG^CCA 1474 TGGCCA BamHI 2430 GGATCC G^GATCC 2945 GGATCC BlpI 1418 GCTCAGC GC^T[ACGT]AGC 3076 GCTAAGC Bpu1102I 1418 GCTCAGC GC^T[ACGT]AGC 3076 GCTAAGC BspHI 421 TCATGA T^CATGA 4017 TCATGA BstAPI 38 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3149 GCATCATATGC BstEII 3349 GGTCACC G^GT[ACGT]ACC 5636 GGTTACC BstPI 3349 GGTCACC G^GT[ACGT]ACC 5636 GGTTACC CelII 1418 GCTCAGC GC^T[ACGT]AGC 3076 GCTAAGC DrdI 1160 GACAGTCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2562 GACAGCGTTGTC EcoO65I 3349 GGTCACC G^GT[ACGT]ACC 5636 GGTTACC EcoRV 1833 GATATC GAT^ATC 2292 GATATC EcoT22I 3147 ATGCAT ATGCA^T 4752 ATGCAT HincII 2076 GTTGAC GT[CT]^[GA]AC 5291 GTTGAC HindII 2076 GTTGAC GT[CT]^[GA]AC 5291 GTTGAC MaeII 2232 ACGT A^CGT 5223 ACGT MluNI 541 TGGCCA TGG^CCA 1474 TGGCCA MscI 541 TGGCCA TGG^CCA 1474 TGGCCA NaeI 31 GCCGGC GCC^GGC 4486 GCCGGC NgoMIV 31 GCCGGC G^CCGGC 4486 GCCGGC NsiI 3147 ATGCAT ATGCA^T 4752 ATGCAT RcaI 421 TCATGA T^CATGA 4017 TCATGA ScaI 1630 AGTACT AGT^ACT 5612 AGTACT SfiI 1269 GGCCTCAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 3891 GGCCACTATGGCC SgrAI 30 CGCCGGCG C[GA]^CCGG[CT]G 380 CGCCGGTG SmaI 669 CCCGGG CCC^GGG 1221 CCCGGG StuI 2309 AGGCCT AGG^CCT 4378 AGGCCT XcmI 529 CCAAGGGCCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1462 CCAGCTCTCCTTTGG XmaCI 669 CCCGGG C^CCGGG 1221 CCCGGG XmaI 669 CCCGGG C^CCGGG 1221 CCCGGG XmnI 2777 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3609 GAAGCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 137 enzymes) # ######################################### Acc65I AccI AccII AccIII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI Asp718I AspHI AvaI AvaII BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KpnI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MroI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XhoII