##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
ApaI AscI Asp718I AvrII BbrPI
BcgI BlnI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BsrBI
BsrGI BssHII BstBI BstUI ClaI
CpoI EagI EclXI Eco52I FseI
HpaI KpnI KspI MfeI MluI
MroI MunI MvnI NaeI NdeI
NgoMIV NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SfiI
SfuI SgrAI SnaBI SspBI SwaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 2216 GACGTC GACGT^C
Aor51HI 2691 AGCGCT AGC^GCT
AviII 1326 TGCGCA TGC^GCA
BalI 606 TGGCCA TGG^CCA
BamHI 3423 GGATCC G^GATCC
BbvCI 4282 CCTCAGC CCTCAGC(-5/-2)
BsaAI 1517 TACGTG [CT]AC^GT[GA]
BsaBI 3424 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1078 CGGTCG CG[GA][CT]^CG
BspMI 6225 GCAGGT ACCTGC(4/8)
BsrFI 1224 ACCGGC [GA]^CCGG[CT]
BssSI 2800 CTCGTG CACGAG(-5/-1)
Bst1107I 4412 GTATAC GTA^TAC
BstAPI 6240 GCAGCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 4412 GTATAC GTA^TAC
Cfr10I 1224 ACCGGC [GA]^CCGG[CT]
DrdI 437 GACAGCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2691 AGCGCT AGC^GCT
EcoRI 3189 GAATTC G^AATTC
EcoT22I 4641 ATGCAT ATGCA^T
FspI 1326 TGCGCA TGC^GCA
HgaI 2204 GCGTC GACGC(5/10)
MamI 3424 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 606 TGGCCA TGG^CCA
MscI 606 TGGCCA TGG^CCA
NsiI 4641 ATGCAT ATGCA^T
PaeR7I 1386 CTCGAG C^TCGAG
PvuII 6459 CAGCTG CAG^CTG
SbfI 4866 CCTGCAGG CCTGCA^GG
SmaI 2701 CCCGGG CCC^GGG
SpeI 4360 ACTAGT A^CTAGT
Sse8387I 4866 CCTGCAGG CCTGCA^GG
SspI 6815 AATATT AAT^ATT
XhoI 1386 CTCGAG C^TCGAG
XmaCI 2701 CCCGGG C^CCGGG
XmaI 2701 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 4268 GTAGAC GT^[AC][GT]AC
4412 GTATAC
AflII 4626 CTTAAG C^TTAAG
6395 CTTAAG
AflIII 487 ACATGT A^C[GA][CT]GT
4224 ACATGT
Alw44I 4220 GTGCAC G^TGCAC
6523 GTGCAC
ApaLI 4220 GTGCAC G^TGCAC
6523 GTGCAC
AspI 2647 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3606 GACTCAGTC
BbeI 1851 GGCGCC GGCGC^C
6015 GGCGCC
BciVI 5477 GGATAC GTATCC(6/5)
4212 GTATCC
BfrI 4626 CTTAAG C^TTAAG
6395 CTTAAG
BglI 1257 GCCGTTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5511 GCCCTGTTGGC
BglII 2410 AGATCT A^GATCT
3660 AGATCT
BspLU11I 487 ACATGT A^CATGT
4224 ACATGT
BsrDI 37 CATTGC GCAATG(2/0)
2664 GCAATG
EcoNI 3112 CCTGGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3596 CCTCCCCCAGG
KasI 1851 GGCGCC G^GCGCC
6015 GGCGCC
NarI 1851 GGCGCC GG^CGCC
6015 GGCGCC
NheI 3009 GCTAGC G^CTAGC
3896 GCTAGC
PflFI 2647 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3606 GACTCAGTC
SapI 2551 GAAGAGC GCTCTTC(1/4)
819 GCTCTTC
ScaI 2721 AGTACT AGT^ACT
6030 AGTACT
SexAI 10 ACCTGGT A^CC[AT]GGT
720 ACCAGGT
SfoI 1851 GGCGCC GGC^GCC
6015 GGCGCC
StuI 4237 AGGCCT AGG^CCT
6087 AGGCCT
Tth111I 2647 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3606 GACTCAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AciI AcsI AcyI AfaI AhdI
AluI AlwI AlwNI AocI ApoI
AseI AsnI Asp700I AspEI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BclI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BsrI BssKI
BstEII BstF5I BstNI BstPI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI Eam1105I EarI
Eco81I EcoO109I EcoO65I EcoRII EcoRV
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PshAI PshBI PstI RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XcmI XhoII XmnI