################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AcsI AgeI Aor51HI ApoI AscI AspI AviII BbeI BbrPI BglII BsaBI BsiEI BsiWI BsmBI BsmI Bsp1407I BspHI BsrGI BssHII Bst1107I BstBI BstZ17I CpoI DrdI EagI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KasI KspI MamI MfeI MluI MunI NarI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PvuI RcaI RsrII SacII SalI SexAI SfoI SfuI SgrAI SphI SspBI SspI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 3183 GACGTC GACGT^C AccI 635 GTAGAC GT^[AC][GT]AC AccII 2942 CGCG CG^CG AccIII 3639 TCCGGA T^CCGGA AclI 4956 AACGTT AA^CGTT AflII 3509 CTTAAG C^TTAAG AflIII 591 ACATGT A^C[GA][CT]GT AhdI 5350 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1187 GTGCAC G^TGCAC ApaLI 1187 GTGCAC G^TGCAC AseI 5553 ATTAAT AT^TAAT AsnI 5553 ATTAAT AT^TAAT Asp700I 5611 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 5350 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 4508 CCTAGG C^CTAGG BamHI 1318 GGATCC G^GATCC BciVI 2335 GGATAC GTATCC(6/5) BclI 5253 TGATCA T^GATCA BfrI 3509 CTTAAG C^TTAAG BlnI 4508 CCTAGG C^CTAGG BsaAI 2317 TACGTA [CT]AC^GT[GA] BsaWI 3639 TCCGGA [AT]^CCGG[AT] BseAI 3639 TCCGGA T^CCGGA BspDI 3668 ATCGAT AT^CGAT BspEI 3639 TCCGGA T^CCGGA BspLU11I 591 ACATGT A^CATGT BsrBI 4985 CCGCTC CCGCTC(-3/-3) BsrDI 1426 CATTGC GCAATG(2/0) BssSI 4004 CTCGTG CACGAG(-5/-1) BstAPI 4261 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 730 GGTCACC G^GT[ACGT]ACC BstPI 730 GGTCACC G^GT[ACGT]ACC BstUI 2942 CGCG CG^CG ClaI 3668 ATCGAT AT^CGAT DraIII 1339 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 5350 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 730 GGTCACC G^GT[ACGT]ACC FbaI 5253 TGATCA T^GATCA MroI 3639 TCCGGA T^CCGGA MvnI 2942 CGCG CG^CG NaeI 114 GCCGGC GCC^GGC NdeI 1742 CATATG CA^TATG NgoMIV 114 GCCGGC G^CCGGC PaeR7I 776 CTCGAG C^TCGAG PshBI 5553 ATTAAT AT^TAAT Psp1406I 4956 AACGTT AA^CGTT SbfI 1606 CCTGCAGG CCTGCA^GG SfiI 1327 GGCCTCTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SnaBI 2317 TACGTA TAC^GTA Sse8387I 1606 CCTGCAGG CCTGCA^GG XbaI 4276 TCTAGA T^CTAGA XhoI 776 CTCGAG C^TCGAG XmnI 5611 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcyI 1009 GGCGTC G[GA]^CG[CT]C 3183 GACGTC BcgI 5025 CGAGGCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4850 GCAGCCTGCTCG BlpI 3167 GCTGAGC GC^T[ACGT]AGC 4979 GCTGAGC Bpu1102I 3167 GCTGAGC GC^T[ACGT]AGC 4979 GCTGAGC BsaHI 1009 GGCGTC G[GA]^CG[CT]C 3183 GACGTC BstXI 2364 CCAAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3780 CCAAGGCTGTGG BstYI 1318 GGATCC [GA]^GATC[CT] 2959 GGATCT CelII 3167 GCTGAGC GC^T[ACGT]AGC 4979 GCTGAGC DraI 5179 TTTAAA TTT^AAA 5898 TTTAAA Hin1I 1009 GGCGTC G[GA]^CG[CT]C 3183 GACGTC HincII 843 GTCAAC GT[CT]^[GA]AC 5097 GTCAAC HindII 843 GTCAAC GT[CT]^[GA]AC 5097 GTCAAC MflI 1318 GGATCC [GA]^GATC[CT] 2959 GGATCT NheI 4053 GCTAGC G^CTAGC 4106 GCTAGC NspI 477 ACATGC [GA]CATG^[CT] 591 ACATGT ScaI 3996 AGTACT AGT^ACT 5538 AGTACT SpeI 5496 ACTAGT A^CTAGT 5522 ACTAGT Tsp509I 3691 AATT ^AATT 4596 AATT XhoII 1318 GGATCC [GA]^GATC[CT] 2959 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### Acc65I AciI AfaI AluI AlwI AlwNI AocI ApaI Asp718I AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PshAI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XcmI XmaCI XmaI