##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AcsI AgeI Aor51HI ApoI AscI
AspI AviII BbeI BbrPI BglII
BsaBI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspHI BsrGI BssHII Bst1107I
BstBI BstZ17I CpoI DrdI EagI
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
KasI KspI MamI MfeI MluI
MunI NarI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PvuI RcaI RsrII
SacII SalI SexAI SfoI SfuI
SgrAI SphI SspBI SspI SwaI
Tth111I
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 3183 GACGTC GACGT^C
AccI 635 GTAGAC GT^[AC][GT]AC
AccII 2942 CGCG CG^CG
AccIII 3639 TCCGGA T^CCGGA
AclI 4956 AACGTT AA^CGTT
AflII 3509 CTTAAG C^TTAAG
AflIII 591 ACATGT A^C[GA][CT]GT
AhdI 5350 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1187 GTGCAC G^TGCAC
ApaLI 1187 GTGCAC G^TGCAC
AseI 5553 ATTAAT AT^TAAT
AsnI 5553 ATTAAT AT^TAAT
Asp700I 5611 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 5350 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4508 CCTAGG C^CTAGG
BamHI 1318 GGATCC G^GATCC
BciVI 2335 GGATAC GTATCC(6/5)
BclI 5253 TGATCA T^GATCA
BfrI 3509 CTTAAG C^TTAAG
BlnI 4508 CCTAGG C^CTAGG
BsaAI 2317 TACGTA [CT]AC^GT[GA]
BsaWI 3639 TCCGGA [AT]^CCGG[AT]
BseAI 3639 TCCGGA T^CCGGA
BspDI 3668 ATCGAT AT^CGAT
BspEI 3639 TCCGGA T^CCGGA
BspLU11I 591 ACATGT A^CATGT
BsrBI 4985 CCGCTC CCGCTC(-3/-3)
BsrDI 1426 CATTGC GCAATG(2/0)
BssSI 4004 CTCGTG CACGAG(-5/-1)
BstAPI 4261 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 730 GGTCACC G^GT[ACGT]ACC
BstPI 730 GGTCACC G^GT[ACGT]ACC
BstUI 2942 CGCG CG^CG
ClaI 3668 ATCGAT AT^CGAT
DraIII 1339 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 5350 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 730 GGTCACC G^GT[ACGT]ACC
FbaI 5253 TGATCA T^GATCA
MroI 3639 TCCGGA T^CCGGA
MvnI 2942 CGCG CG^CG
NaeI 114 GCCGGC GCC^GGC
NdeI 1742 CATATG CA^TATG
NgoMIV 114 GCCGGC G^CCGGC
PaeR7I 776 CTCGAG C^TCGAG
PshBI 5553 ATTAAT AT^TAAT
Psp1406I 4956 AACGTT AA^CGTT
SbfI 1606 CCTGCAGG CCTGCA^GG
SfiI 1327 GGCCTCTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 2317 TACGTA TAC^GTA
Sse8387I 1606 CCTGCAGG CCTGCA^GG
XbaI 4276 TCTAGA T^CTAGA
XhoI 776 CTCGAG C^TCGAG
XmnI 5611 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 1009 GGCGTC G[GA]^CG[CT]C
3183 GACGTC
BcgI 5025 CGAGGCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4850 GCAGCCTGCTCG
BlpI 3167 GCTGAGC GC^T[ACGT]AGC
4979 GCTGAGC
Bpu1102I 3167 GCTGAGC GC^T[ACGT]AGC
4979 GCTGAGC
BsaHI 1009 GGCGTC G[GA]^CG[CT]C
3183 GACGTC
BstXI 2364 CCAAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3780 CCAAGGCTGTGG
BstYI 1318 GGATCC [GA]^GATC[CT]
2959 GGATCT
CelII 3167 GCTGAGC GC^T[ACGT]AGC
4979 GCTGAGC
DraI 5179 TTTAAA TTT^AAA
5898 TTTAAA
Hin1I 1009 GGCGTC G[GA]^CG[CT]C
3183 GACGTC
HincII 843 GTCAAC GT[CT]^[GA]AC
5097 GTCAAC
HindII 843 GTCAAC GT[CT]^[GA]AC
5097 GTCAAC
MflI 1318 GGATCC [GA]^GATC[CT]
2959 GGATCT
NheI 4053 GCTAGC G^CTAGC
4106 GCTAGC
NspI 477 ACATGC [GA]CATG^[CT]
591 ACATGT
ScaI 3996 AGTACT AGT^ACT
5538 AGTACT
SpeI 5496 ACTAGT A^CTAGT
5522 ACTAGT
Tsp509I 3691 AATT ^AATT
4596 AATT
XhoII 1318 GGATCC [GA]^GATC[CT]
2959 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
Acc65I AciI AfaI AluI AlwI
AlwNI AocI ApaI Asp718I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PshAI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I TspRI
TthHB8I Van91I XcmI XmaCI XmaI