##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
Acc65I AclI AflII AhdI AscI
Asp718I AspEI AviII AvrII BciVI
BfrI BglI BglII BlnI BspDI
BssHII ClaI Eam1105I FseI FspI
KpnI KspI MluI NheI NotI
NruI PacI PaeR7I PmeI Psp1406I
PvuI SacI SacII SalI SbfI
ScaI SexAI SfiI SgrAI SmlI
SnaBI SpeI Sse8387I SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 153 TCCGGA T^CCGGA
AgeI 158 ACCGGT A^CCGGT
Alw44I 3471 GTGCAC G^TGCAC
Aor51HI 3041 AGCGCT AGC^GCT
ApaLI 3471 GTGCAC G^TGCAC
Asp700I 2585 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 5392 CCTCAGC CCTCAGC(-5/-2)
BcgI 4098 GCAACTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 6231 TGATCA T^GATCA
BlpI 244 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 244 GCTCAGC GC^T[ACGT]AGC
BseAI 153 TCCGGA T^CCGGA
BsiWI 1470 CGTACG C^GTACG
BspEI 153 TCCGGA T^CCGGA
BspHI 1583 TCATGA T^CATGA
BstBI 2582 TTCGAA TT^CGAA
CelII 244 GCTCAGC GC^T[ACGT]AGC
CpoI 155 CGGACCG CG^G[AT]CCG
DraIII 5422 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4961 GACCACACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3041 AGCGCT AGC^GCT
EcoRV 2119 GATATC GAT^ATC
FbaI 6231 TGATCA T^GATCA
MfeI 1056 CAATTG C^AATTG
MroI 153 TCCGGA T^CCGGA
MunI 1056 CAATTG C^AATTG
NspV 2582 TTCGAA TT^CGAA
PinAI 158 ACCGGT A^CCGGT
RcaI 1583 TCATGA T^CATGA
RsrII 155 CGGACCG CG^G[AT]CCG
SfuI 2582 TTCGAA TT^CGAA
XcmI 3169 CCAGGGTGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2585 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 849 GACGTC GACGT^C
2035 GACGTC
AocI 835 CCTGAGG CC^T[ACGT]AGG
2976 CCTGAGG
AseI 3745 ATTAAT AT^TAAT
6454 ATTAAT
AsnI 3745 ATTAAT AT^TAAT
6454 ATTAAT
BamHI 142 GGATCC G^GATCC
236 GGATCC
BsaBI 4344 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5401 GATCCCTATC
BsmBI 1574 GAGACG CGTCTC(1/5)
2033 GAGACG
BspLU11I 2777 ACATGT A^CATGT
6255 ACATGT
Bst1107I 5734 GTATAC GTA^TAC
5873 GTATAC
BstEII 3556 GGTGACC G^GT[ACGT]ACC
5626 GGTGACC
BstPI 3556 GGTGACC G^GT[ACGT]ACC
5626 GGTGACC
BstZ17I 5734 GTATAC GTA^TAC
5873 GTATAC
Bsu36I 835 CCTGAGG CC^T[ACGT]AGG
2976 CCTGAGG
EagI 230 CGGCCG C^GGCCG
373 CGGCCG
EclXI 230 CGGCCG C^GGCCG
373 CGGCCG
Eco52I 230 CGGCCG C^GGCCG
373 CGGCCG
Eco81I 835 CCTGAGG CC^T[ACGT]AGG
2976 CCTGAGG
EcoO65I 3556 GGTGACC G^GT[ACGT]ACC
5626 GGTGACC
EcoRI 5444 GAATTC G^AATTC
5759 GAATTC
EcoT22I 2373 ATGCAT ATGCA^T
3429 ATGCAT
HgaI 294 GACGC GACGC(5/10)
2572 GACGC
HindIII 2150 AAGCTT A^AGCTT
6520 AAGCTT
HpaI 597 GTTAAC GTT^AAC
6478 GTTAAC
MamI 4344 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5401 GATCCCTATC
NcoI 2897 CCATGG C^CATGG
5809 CCATGG
NdeI 739 CATATG CA^TATG
1979 CATATG
NsiI 2373 ATGCAT ATGCA^T
3429 ATGCAT
PpuMI 220 AGGTCCT [GA]G^G[AT]CC[CT]
2980 AGGACCT
PshAI 170 GACGGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2572 GACGCTTGTC
PshBI 3745 ATTAAT AT^TAAT
6454 ATTAAT
SapI 5138 GAAGAGC GCTCTTC(1/4)
17 GCTCTTC
SphI 3431 GCATGC GCATG^C
5804 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApaI ApoI AspHI AspI AvaI
AvaII BalI BanI BanII BbeI
BbrPI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PflMI PleI PmaCI PmlI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SspBI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XhoII
XmaCI XmaI