##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
Asp718I BbrPI BcgI BfrI BsaBI
BsiEI BsiWI Bsp1407I BspDI BspLU11I
BsrGI BssHII BstBI ClaI CpoI
DraIII DrdI EagI EclXI Eco52I
EcoRI EcoT22I FseI KpnI MamI
MluI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SbfI ScaI SfiI SfuI SgrAI
SpeI Sse8387I SspBI SwaI XcmI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 5831 GACGTC GACGT^C
AflIII 3093 ACGTGT A^C[GA][CT]GT
AhdI 4083 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4093 CCTCAGG CC^T[ACGT]AGG
AseI 2857 ATTAAT AT^TAAT
AsnI 2857 ATTAAT AT^TAAT
AspEI 4083 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4250 TGCGCA TGC^GCA
AvrII 48 CCTAGG C^CTAGG
BamHI 5296 GGATCC G^GATCC
BglII 3691 AGATCT A^GATCT
BlnI 48 CCTAGG C^CTAGG
BlpI 2131 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2131 GCTGAGC GC^T[ACGT]AGC
BsmI 2037 GAATGC GAATGC(1/-1)
BspHI 3141 TCATGA T^CATGA
BsrBI 84 CCGCTC CCGCTC(-3/-3)
BssSI 4881 CACGAG CACGAG(-5/-1)
Bst1107I 1472 GTATAC GTA^TAC
BstAPI 5130 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1705 GGTGACC G^GT[ACGT]ACC
BstPI 1705 GGTGACC G^GT[ACGT]ACC
BstZ17I 1472 GTATAC GTA^TAC
Bsu36I 4093 CCTCAGG CC^T[ACGT]AGG
CelII 2131 GCTGAGC GC^T[ACGT]AGC
Eam1105I 4083 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4093 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1705 GGTGACC G^GT[ACGT]ACC
EcoRV 1218 GATATC GAT^ATC
FspI 4250 TGCGCA TGC^GCA
KspI 177 CCGCGG CCGC^GG
MfeI 1274 CAATTG C^AATTG
MunI 1274 CAATTG C^AATTG
NcoI 5313 CCATGG C^CATGG
PaeR7I 87 CTCGAG C^TCGAG
PshAI 3769 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2857 ATTAAT AT^TAAT
RcaI 3141 TCATGA T^CATGA
SacII 177 CCGCGG CCGC^GG
SalI 141 GTCGAC G^TCGAC
SexAI 3392 ACCAGGT A^CC[AT]GGT
SmaI 164 CCCGGG CCC^GGG
SnaBI 4854 TACGTA TAC^GTA
SspI 5486 AATATT AAT^ATT
XhoI 87 CTCGAG C^TCGAG
XmaCI 164 CCCGGG C^CCGGG
XmaI 164 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 141 GTCGAC GT^[AC][GT]AC
1472 GTATAC
AccII 146 CGCG CG^CG
178 CGCG
AccIII 1907 TCCGGA T^CCGGA
5745 TCCGGA
Alw44I 76 GTGCAC G^TGCAC
4153 GTGCAC
ApaI 161 GGGCCC GGGCC^C
1199 GGGCCC
ApaLI 76 GTGCAC G^TGCAC
4153 GTGCAC
Asp700I 2781 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5734 GAACAACTTC
AspI 1930 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5831 GACGTCGTC
BanI 5624 GGCGCC G^G[CT][GA]CC
5918 GGCGCC
BbeI 5624 GGCGCC GGCGC^C
5918 GGCGCC
BciVI 3366 GTATCC GTATCC(6/5)
6194 GTATCC
BclI 2330 TGATCA T^GATCA
5849 TGATCA
BglI 2384 GCCCCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5627 GCCTGCCTGGC
BsaAI 3092 TACGTG [CT]AC^GT[GA]
4854 TACGTA
BseAI 1907 TCCGGA T^CCGGA
5745 TCCGGA
BsmBI 2826 CGTCTC CGTCTC(1/5)
4649 GAGACG
BspEI 1907 TCCGGA T^CCGGA
5745 TCCGGA
BstUI 146 CGCG CG^CG
178 CGCG
EcoNI 1314 CCTCAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4236 CCTAAGCCAGG
FbaI 2330 TGATCA T^GATCA
5849 TGATCA
HindIII 1162 AAGCTT A^AGCTT
3889 AAGCTT
HpaI 2890 GTTAAC GTT^AAC
5572 GTTAAC
KasI 5624 GGCGCC G^GCGCC
5918 GGCGCC
MroI 1907 TCCGGA T^CCGGA
5745 TCCGGA
MvnI 146 CGCG CG^CG
178 CGCG
NaeI 4207 GCCGGC GCC^GGC
4349 GCCGGC
NarI 5624 GGCGCC GG^CGCC
5918 GGCGCC
NgoMIV 4207 GCCGGC G^CCGGC
4349 GCCGGC
PflFI 1930 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5831 GACGTCGTC
PflMI 233 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1752 CCAGGGCCTGG
SapI 1021 GCTCTTC GCTCTTC(1/4)
3925 GCTCTTC
SfoI 5624 GGCGCC GGC^GCC
5918 GGCGCC
StuI 3955 AGGCCT AGG^CCT
5349 AGGCCT
Tth111I 1930 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5831 GACGTCGTC
Van91I 233 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1752 CCAGGGCCTGG
XmnI 2781 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5734 GAACAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApoI AspHI
AvaI AvaII BalI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco47III EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII