##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
Acc65I AclI AflII AgeI Aor51HI
AscI AseI AsnI Asp718I AviII
BcgI BciVI BfrI BglII BsaBI
BsiWI BspDI BspLU11I BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DrdI Eco47III EcoRI EcoRV FseI
FspI HindIII KpnI MamI MfeI
MunI NotI NruI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SalI SexAI SfuI SgrAI
SnaBI SphI SwaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 5648 GACGTC GACGT^C
AccIII 1832 TCCGGA T^CCGGA
AcsI 1918 AAATTC [GA]^AATT[CT]
AhdI 5221 GACCTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 1918 AAATTC [GA]^AATT[CT]
Asp700I 4752 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 5221 GACCTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 5003 CACGTG CAC^GTG
BclI 2547 TGATCA T^GATCA
BsaWI 1832 TCCGGA [AT]^CCGG[AT]
BseAI 1832 TCCGGA T^CCGGA
BsmBI 3957 GAGACG CGTCTC(1/5)
BspEI 1832 TCCGGA T^CCGGA
BssSI 428 CTCGTG CACGAG(-5/-1)
BstAPI 2932 GCATAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI 135 CGGCCG C^GGCCG
Eam1105I 5221 GACCTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 135 CGGCCG C^GGCCG
Eco52I 135 CGGCCG C^GGCCG
EcoT22I 443 ATGCAT ATGCA^T
FbaI 2547 TGATCA T^GATCA
HpaI 4859 GTTAAC GTT^AAC
KspI 67 CCGCGG CCGC^GG
MluI 234 ACGCGT A^CGCGT
MroI 1832 TCCGGA T^CCGGA
NsiI 443 ATGCAT ATGCA^T
PaeR7I 1954 CTCGAG C^TCGAG
PmaCI 5003 CACGTG CAC^GTG
PmlI 5003 CACGTG CAC^GTG
PshAI 5648 GACGTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 67 CCGCGG CCGC^GG
SbfI 2075 CCTGCAGG CCTGCA^GG
ScaI 3888 AGTACT AGT^ACT
Sse8387I 2075 CCTGCAGG CCTGCA^GG
SspI 5632 AATATT AAT^ATT
StuI 4841 AGGCCT AGG^CCT
XbaI 4601 TCTAGA T^CTAGA
XhoI 1954 CTCGAG C^TCGAG
XmnI 4752 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 902 GTCTAC GT^[AC][GT]AC
4517 GTAGAC
AflIII 234 ACGCGT A^C[GA][CT]GT
1656 ACGTGT
Alw44I 4510 GTGCAC G^TGCAC
5179 GTGCAC
ApaLI 4510 GTGCAC G^TGCAC
5179 GTGCAC
AspI 500 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5596 GACAGGGTC
BbeI 215 GGCGCC GGCGC^C
1515 GGCGCC
BsaAI 5003 CACGTG [CT]AC^GT[GA]
6110 CACGTA
BsiEI 18 CGGTCG CG[GA][CT]^CG
135 CGGCCG
BsmI 2809 GAATGC GAATGC(1/-1)
5398 GCATTC
BspHI 2586 TCATGA T^CATGA
3904 TCATGA
BsrDI 2679 CATTGC GCAATG(2/0)
4437 CATTGC
KasI 215 GGCGCC G^GCGCC
1515 GGCGCC
NarI 215 GGCGCC GG^CGCC
1515 GGCGCC
NdeI 2188 CATATG CA^TATG
3573 CATATG
NheI 3151 GCTAGC G^CTAGC
3710 GCTAGC
PflFI 500 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5596 GACAGGGTC
RcaI 2586 TCATGA T^CATGA
3904 TCATGA
SapI 1104 GCTCTTC GCTCTTC(1/4)
1468 GCTCTTC
SfoI 215 GGCGCC GGC^GCC
1515 GGCGCC
SmaI 914 CCCGGG CCC^GGG
1059 CCCGGG
SpeI 887 ACTAGT A^CTAGT
2993 ACTAGT
Tth111I 500 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5596 GACAGGGTC
XmaCI 914 CCCGGG C^CCGGG
1059 CCCGGG
XmaI 914 CCCGGG C^CCGGG
1059 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII AvrII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrBI BsrFI
BsrGI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII