################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## Acc65I AclI AflII AgeI Aor51HI AscI AseI AsnI Asp718I AviII BcgI BciVI BfrI BglII BsaBI BsiWI BspDI BspLU11I BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI Eco47III EcoRI EcoRV FseI FspI HindIII KpnI MamI MfeI MunI NotI NruI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RsrII SalI SexAI SfuI SgrAI SnaBI SphI SwaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 5648 GACGTC GACGT^C AccIII 1832 TCCGGA T^CCGGA AcsI 1918 AAATTC [GA]^AATT[CT] AhdI 5221 GACCTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 1918 AAATTC [GA]^AATT[CT] Asp700I 4752 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 5221 GACCTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 5003 CACGTG CAC^GTG BclI 2547 TGATCA T^GATCA BsaWI 1832 TCCGGA [AT]^CCGG[AT] BseAI 1832 TCCGGA T^CCGGA BsmBI 3957 GAGACG CGTCTC(1/5) BspEI 1832 TCCGGA T^CCGGA BssSI 428 CTCGTG CACGAG(-5/-1) BstAPI 2932 GCATAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC EagI 135 CGGCCG C^GGCCG Eam1105I 5221 GACCTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 135 CGGCCG C^GGCCG Eco52I 135 CGGCCG C^GGCCG EcoT22I 443 ATGCAT ATGCA^T FbaI 2547 TGATCA T^GATCA HpaI 4859 GTTAAC GTT^AAC KspI 67 CCGCGG CCGC^GG MluI 234 ACGCGT A^CGCGT MroI 1832 TCCGGA T^CCGGA NsiI 443 ATGCAT ATGCA^T PaeR7I 1954 CTCGAG C^TCGAG PmaCI 5003 CACGTG CAC^GTG PmlI 5003 CACGTG CAC^GTG PshAI 5648 GACGTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 67 CCGCGG CCGC^GG SbfI 2075 CCTGCAGG CCTGCA^GG ScaI 3888 AGTACT AGT^ACT Sse8387I 2075 CCTGCAGG CCTGCA^GG SspI 5632 AATATT AAT^ATT StuI 4841 AGGCCT AGG^CCT XbaI 4601 TCTAGA T^CTAGA XhoI 1954 CTCGAG C^TCGAG XmnI 4752 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 902 GTCTAC GT^[AC][GT]AC 4517 GTAGAC AflIII 234 ACGCGT A^C[GA][CT]GT 1656 ACGTGT Alw44I 4510 GTGCAC G^TGCAC 5179 GTGCAC ApaLI 4510 GTGCAC G^TGCAC 5179 GTGCAC AspI 500 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 5596 GACAGGGTC BbeI 215 GGCGCC GGCGC^C 1515 GGCGCC BsaAI 5003 CACGTG [CT]AC^GT[GA] 6110 CACGTA BsiEI 18 CGGTCG CG[GA][CT]^CG 135 CGGCCG BsmI 2809 GAATGC GAATGC(1/-1) 5398 GCATTC BspHI 2586 TCATGA T^CATGA 3904 TCATGA BsrDI 2679 CATTGC GCAATG(2/0) 4437 CATTGC KasI 215 GGCGCC G^GCGCC 1515 GGCGCC NarI 215 GGCGCC GG^CGCC 1515 GGCGCC NdeI 2188 CATATG CA^TATG 3573 CATATG NheI 3151 GCTAGC G^CTAGC 3710 GCTAGC PflFI 500 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 5596 GACAGGGTC RcaI 2586 TCATGA T^CATGA 3904 TCATGA SapI 1104 GCTCTTC GCTCTTC(1/4) 1468 GCTCTTC SfoI 215 GGCGCC GGC^GCC 1515 GGCGCC SmaI 914 CCCGGG CCC^GGG 1059 CCCGGG SpeI 887 ACTAGT A^CTAGT 2993 ACTAGT Tth111I 500 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 5596 GACAGGGTC XmaCI 914 CCCGGG C^CCGGG 1059 CCCGGG XmaI 914 CCCGGG C^CCGGG 1059 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 142 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII AvrII BalI BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrFI BsrGI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII