##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AccIII AclI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AvrII BbeI BbrPI
BciVI BlnI BsaAI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I FseI HincII HindII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NarI NotI
NruI NspV PacI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SspBI SspI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AatII 988 GACGTC GACGT^C
AccI 1821 GTCTAC GT^[AC][GT]AC
AccII 1465 CGCG CG^CG
AcyI 988 GACGTC G[GA]^CG[CT]C
AflII 4197 CTTAAG C^TTAAG
AgeI 838 ACCGGT A^CCGGT
Asp700I 2583 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 65 TGCGCA TGC^GCA
BfrI 4197 CTTAAG C^TTAAG
BsaBI 5545 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 988 GACGTC G[GA]^CG[CT]C
BsrBI 4577 CCGCTC CCGCTC(-3/-3)
BssSI 1492 CTCGTG CACGAG(-5/-1)
BstUI 1465 CGCG CG^CG
EcoRI 1883 GAATTC G^AATTC
EcoRV 1359 GATATC GAT^ATC
EcoT22I 6363 ATGCAT ATGCA^T
FspI 65 TGCGCA TGC^GCA
Hin1I 988 GACGTC G[GA]^CG[CT]C
MamI 5545 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1465 CGCG CG^CG
NaeI 35 GCCGGC GCC^GGC
NcoI 3144 CCATGG C^CATGG
NgoMIV 35 GCCGGC G^CCGGC
NsiI 6363 ATGCAT ATGCA^T
PinAI 838 ACCGGT A^CCGGT
PshAI 1950 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 1085 ACCTGGT A^CC[AT]GGT
SpeI 3186 ACTAGT A^CTAGT
SphI 4673 GCATGC GCATG^C
SwaI 4910 ATTTAAAT ATTT^AAAT
XmnI 2583 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspI 3901 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5017 GACCTTGTC
BalI 368 TGGCCA TGG^CCA
3476 TGGCCA
BclI 3195 TGATCA T^GATCA
5497 TGATCA
BglI 3743 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4837 GCCTCCAAGGC
BglII 4870 AGATCT A^GATCT
6346 AGATCT
BsiEI 4628 CGACCG CG[GA][CT]^CG
5540 CGGTCG
BsmI 981 GAATGC GAATGC(1/-1)
5667 GCATTC
BsrDI 6366 CATTGC GCAATG(2/0)
2634 GCAATG
DraIII 4368 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
6269 CACAAGGTG
DrdI 1177 GACACGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5696 GACTCCCAGGTC
DsaI 257 CCGTGG C^C[GA][CT]GG
3144 CCATGG
EaeI 368 TGGCCA [CT]^GGCC[GA]
3476 TGGCCA
FbaI 3195 TGATCA T^GATCA
5497 TGATCA
HgaI 5678 GACGC GACGC(5/10)
1734 GCGTC
HindIII 5944 AAGCTT A^AGCTT
6138 AAGCTT
MluNI 368 TGGCCA TGG^CCA
3476 TGGCCA
MscI 368 TGGCCA TGG^CCA
3476 TGGCCA
PflFI 3901 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5017 GACCTTGTC
SapI 1072 GAAGAGC GCTCTTC(1/4)
5684 GAAGAGC
Tth111I 3901 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5017 GACCTTGTC
XbaI 345 TCTAGA T^CTAGA
3495 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BamHI BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspLU11I
BspMI BsrFI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeI NdeII NheI NlaIII NlaIV
NspI PaeR7I PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoI XhoII