##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BglII BlnI BlpI
Bpu1102I BseAI BsiWI BspDI BspEI
BspHI BsrDI BsrFI BssSI BstBI
CelII Cfr10I ClaI DraIII DrdI
Eam1105I Eco47III FseI FspI KasI
KpnI MluI MroI NaeI NarI
NgoMIV NheI NruI NspV PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PvuI RcaI SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I SspI SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AclI 351 AACGTT AA^CGTT
AcyI 2276 GACGCC G[GA]^CG[CT]C
AflII 3337 CTTAAG C^TTAAG
AflIII 2789 ACATGT A^C[GA][CT]GT
ApaI 3361 GGGCCC GGGCC^C
BalI 3510 TGGCCA TGG^CCA
BcgI 853 GCACATACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 6155 TGATCA T^GATCA
BfrI 3337 CTTAAG C^TTAAG
BglI 131 GCCGCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1597 TACGTA [CT]AC^GT[GA]
BsaHI 2276 GACGCC G[GA]^CG[CT]C
BsaI 5491 GGTCTC GGTCTC(1/5)
BsaWI 10 ACCGGA [AT]^CCGG[AT]
BspLU11I 2789 ACATGT A^CATGT
BssHII 31 GCGCGC G^CGCGC
BstEII 1247 GGTCACC G^GT[ACGT]ACC
BstPI 1247 GGTCACC G^GT[ACGT]ACC
CpoI 98 CGGACCG CG^G[AT]CCG
EcoO65I 1247 GGTCACC G^GT[ACGT]ACC
EcoRV 2267 GATATC GAT^ATC
FbaI 6155 TGATCA T^GATCA
HaeII 2257 AGCGCC [GA]GCGC^[CT]
Hin1I 2276 GACGCC G[GA]^CG[CT]C
HindIII 4771 AAGCTT A^AGCTT
KspI 132 CCGCGG CCGC^GG
MluNI 3510 TGGCCA TGG^CCA
MscI 3510 TGGCCA TGG^CCA
NotI 128 GCGGCCGC GC^GGCCGC
PacI 6186 TTAATTAA TTAAT^TAA
PshAI 4538 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 351 AACGTT AA^CGTT
RsrII 98 CGGACCG CG^G[AT]CCG
SacII 132 CCGCGG CCGC^GG
ScaI 5503 AGTACT AGT^ACT
SexAI 2466 ACCAGGT A^CC[AT]GGT
SnaBI 1597 TACGTA TAC^GTA
StuI 4657 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 32 CGCG CG^CG
133 CGCG
AvaI 1644 CTCGGG C^[CT]CG[GA]G
2340 CCCGAG
BanI 2579 GGTGCC G^G[CT][GA]CC
4383 GGTGCC
BbsI 482 GAAGAC GAAGAC(2/6)
1020 GAAGAC
BbvCI 623 CCTCAGC CCTCAGC(-5/-2)
767 CCTCAGC
BpmI 678 CTCCAG CTGGAG(16/14)
2060 CTCCAG
BpuAI 482 GAAGAC GAAGAC(2/6)
1020 GAAGAC
BsaBI 4987 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5312 GATTCTGATC
BsiEI 50 CGGCCG CG[GA][CT]^CG
129 CGGCCG
BsmBI 1978 GAGACG CGTCTC(1/5)
3545 GAGACG
BsoBI 1644 CTCGGG C^[CT]CG[GA]G
2340 CCCGAG
BsrBI 126 GAGCGG CCGCTC(-3/-3)
1931 GAGCGG
BstAPI 2285 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3059 GCATATTGTGC
BstUI 32 CGCG CG^CG
133 CGCG
BstXI 3568 CCATTGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4297 CCATGGACTTGG
EagI 50 CGGCCG C^GGCCG
129 CGGCCG
EclXI 50 CGGCCG C^GGCCG
129 CGGCCG
Eco52I 50 CGGCCG C^GGCCG
129 CGGCCG
EcoT22I 4646 ATGCAT ATGCA^T
5369 ATGCAT
HgaI 2276 GACGC GACGC(5/10)
1087 GCGTC
MamI 4987 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5312 GATTCTGATC
MvnI 32 CGCG CG^CG
133 CGCG
NcoI 1043 CCATGG C^CATGG
4297 CCATGG
NsiI 4646 ATGCAT ATGCA^T
5369 ATGCAT
PflMI 554 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1778 CCAGGCTCTGG
PpuMI 817 AGGTCCT [GA]G^G[AT]CC[CT]
1315 AGGACCT
SacI 564 GAGCTC GAGCT^C
1819 GAGCTC
Van91I 554 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1778 CCAGGCTCTGG
XbaI 412 TCTAGA T^CTAGA
2108 TCTAGA
XcmI 550 CCACCCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2152 CCACATAGAACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AseI
AsnI Asp700I AspHI AvaII BanII
BbvI BciVI BcnI BfaI BmyI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspMI BsrGI BsrI BssKI
Bst1107I BstF5I BstNI BstYI BstZ17I
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MspI MunI
MvaI MwoI NciI NdeI NdeII
NlaIII NlaIV NspI PleI PshBI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI