##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI AlwNI AocI
Aor51HI AscI AseI AsnI Asp718I
AspEI AspI AvrII BalI BbrPI
BbvCI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsiWI BsmBI BsmI
BspDI BspHI BspLU11I BspMI Bst1107I
BstBI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRV EcoT14I FbaI FseI HpaI
KpnI KspI MaeII MamI MfeI
MluI MluNI MscI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI TfiI Tth111I
Van91I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 618 TCCGGA T^CCGGA
AcyI 56 GGCGCC G[GA]^CG[CT]C
ApaI 140 GGGCCC GGGCC^C
Asp700I 1142 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 314 TGCGCA TGC^GCA
BamHI 249 GGATCC G^GATCC
BanI 56 GGCGCC G^G[CT][GA]CC
BanII 140 GGGCCC G[GA]GC[CT]^C
BbeI 56 GGCGCC GGCGC^C
BbsI 1041 GTCTTC GAAGAC(2/6)
BcgI 853 CGAAGAGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 53 CCCGG CC^[GC]GG
BpuAI 1041 GTCTTC GAAGAC(2/6)
BsaHI 56 GGCGCC G[GA]^CG[CT]C
BseAI 618 TCCGGA T^CCGGA
BsiEI 287 CGACCG CG[GA][CT]^CG
Bsp1407I 826 TGTACA T^GTACA
BspEI 618 TCCGGA T^CCGGA
BsrDI 664 CATTGC GCAATG(2/0)
BsrFI 971 ACCGGC [GA]^CCGG[CT]
BsrGI 826 TGTACA T^GTACA
BssHII 134 GCGCGC G^CGCGC
BstEII 76 GGTCACC G^GT[ACGT]ACC
BstPI 76 GGTCACC G^GT[ACGT]ACC
Cfr10I 971 ACCGGC [GA]^CCGG[CT]
DdeI 44 CTAAG C^T[ACGT]AG
EaeI 187 TGGCCG [CT]^GGCC[GA]
EcoNI 240 CCTAACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 76 GGTCACC G^GT[ACGT]ACC
EcoRI 431 GAATTC G^AATTC
EcoT22I 712 ATGCAT ATGCA^T
FspI 314 TGCGCA TGC^GCA
HaeII 56 GGCGCC [GA]GCGC^[CT]
HgaI 176 GACGC GACGC(5/10)
Hin1I 56 GGCGCC G[GA]^CG[CT]C
HindIII 728 AAGCTT A^AGCTT
HinfI 1096 GAGTC G^A[ACGT]TC
KasI 56 GGCGCC G^GCGCC
MroI 618 TCCGGA T^CCGGA
MslI 597 CACCGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 56 GGCGCC GG^CGCC
NciI 53 CCCGG CC^[GC]GG
NsiI 712 ATGCAT ATGCA^T
PleI 1096 GAGTC GAGTC(4/5)
PvuII 1193 CAGCTG CAG^CTG
SfoI 56 GGCGCC GGC^GCC
SmlI 645 CTCAAG C^T[CT][GA]AG
SspBI 826 TGTACA T^GTACA
XbaI 763 TCTAGA T^CTAGA
XmnI 1142 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 431 GAATTC [GA]^AATT[CT]
533 GAATTT
AfaI 468 GTAC GT^AC
827 GTAC
Alw44I 4 GTGCAC G^TGCAC
126 GTGCAC
ApaLI 4 GTGCAC G^TGCAC
126 GTGCAC
ApoI 431 GAATTC [GA]^AATT[CT]
533 GAATTT
AvaI 180 CTCGGG C^[CT]CG[GA]G
209 CTCGGG
AvaII 149 GGACC G^G[AT]CC
626 GGACC
BfaI 169 CTAG C^TAG
764 CTAG
BsaWI 289 ACCGGA [AT]^CCGG[AT]
618 TCCGGA
BseRI 977 GAGGAG GAGGAG(10/8)
980 GAGGAG
BsoBI 180 CTCGGG C^[CT]CG[GA]G
209 CTCGGG
BsrBI 326 GAGCGG CCGCTC(-3/-3)
1101 GAGCGG
BssSI 93 CTCGTG CACGAG(-5/-1)
488 CTCGTG
BstAPI 530 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1029 GCAAGCTGTGC
BstYI 249 GGATCC [GA]^GATC[CT]
589 GGATCT
DraI 817 TTTAAA TTT^AAA
880 TTTAAA
DraII 148 GGGACCC [GA]G^G[ACGT]CC[CT]
625 AGGACCT
EcoO109I 148 GGGACCC [GA]G^G[ACGT]CC[CT]
625 AGGACCT
HincII 449 GTCAAC GT[CT]^[GA]AC
677 GTTGAC
HindII 449 GTCAAC GT[CT]^[GA]AC
677 GTTGAC
MaeI 169 CTAG C^TAG
764 CTAG
MflI 249 GGATCC [GA]^GATC[CT]
589 GGATCT
NlaIII 1093 CATG CATG^
1156 CATG
PpuMI 148 GGGACCC [GA]G^G[AT]CC[CT]
625 AGGACCT
RsaI 468 GTAC GT^AC
827 GTAC
TspRI 7 CACTG CA[GC]TG[ACGT][ACGT]^
1217 CACTG
XhoII 249 GGATCC [GA]^GATC[CT]
589 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
BbvI BmyI BpmI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DpnII
DsaI EarI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TaqI Tru9I TseI Tsp45I Tsp509I
TthHB8I