##################################
# NO CUT ENZYMES (  170 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsgI        BsiHKAI     
BsiWI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        ClaI        CpoI        
DraI        DraIII      DrdI        Eam1105I    Eco47III    
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HaeII       HhaI        HinP1I      HindIII     
HpaI        HphI        KasI        KpnI        KspI        
MaeI        MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MslI        MunI        
MvnI        NarI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tsp509I     Tth111I     Van91I      XbaI        
XcmI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AciI          359  GCGG                 CCGC(-3/-1)
AcyI          421  GGCGTC               G[GA]^CG[CT]C
AlwI           88  GATCC                GGATC(4/5)
AvaI          258  CTCGAG               C^[CT]CG[GA]G
BbvI          101  GCTGC                GCAGC(8/12)
BsaHI         421  GGCGTC               G[GA]^CG[CT]C
BsiEI         172  CGGCCG               CG[GA][CT]^CG
BsmFI         276  GGGAC                GGGAC(10/14)
BsmI           73  GAATGC               GAATGC(1/-1)
BsoBI         258  CTCGAG               C^[CT]CG[GA]G
BsrDI         293  GCAATG               GCAATG(2/0)
BsrFI         170  GCCGGC               [GA]^CCGG[CT]
BsrI           51  ACTGG                ACTGG(1/-1)
BstAPI        161  GCAGACAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I        170  GCCGGC               [GA]^CCGG[CT]
EaeI          172  CGGCCG               [CT]^GGCC[GA]
EagI          172  CGGCCG               C^GGCCG
EarI          111  CTCTTC               CTCTTC(1/4)
EclXI         172  CGGCCG               C^GGCCG
Eco52I        172  CGGCCG               C^GGCCG
HgaI          422  GCGTC                GACGC(5/10)
Hin1I         421  GGCGTC               G[GA]^CG[CT]C
HincII         47  GTCAAC               GT[CT]^[GA]AC
HindII         47  GTCAAC               GT[CT]^[GA]AC
HinfI         477  GATTC                G^A[ACGT]TC
Ksp632I       111  CTCTTC               CTCTTC(1/4)
MaeII         386  ACGT                 A^CGT
MseI          518  TTAA                 T^TAA
MspA1I         99  CAGCTG               C[AC]G^C[GT]G
NaeI          170  GCCGGC               GCC^GGC
NgoMIV        170  GCCGGC               G^CCGGC
PaeR7I        258  CTCGAG               C^TCGAG
PvuII          99  CAGCTG               CAG^CTG
SfaNI          23  GATGC                GCATC(5/9)
SmlI          258  CTCGAG               C^T[CT][GA]AG
TfiI          477  GATTC                G^A[AT]TC
Tru9I         518  TTAA                 T^TAA
TseI          101  GCTGC                G^C[AT]GC
TspRI         166  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoI          258  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AvaII         179  GGTCC                G^G[AT]CC
              277  GGACC               
BanII         215  GGGCTC               G[GA]GC[CT]^C
              255  GGGCTC              
BcnI           91  CCGGG                CC^[GC]GG
              175  CCGGG               
BmyI          215  GGGCTC               G[AGT]GC[ACT]^C
              255  GGGCTC              
BseRI         131  CTCCTC               GAGGAG(10/8)
              486  CTCCTC              
BsmBI         378  GAGACG               CGTCTC(1/5)
              383  GAGACG              
Bsp1286I      215  GGGCTC               G[AGT]GC[ACT]^C
              255  GGGCTC              
DdeI          443  CTGAG                C^T[ACGT]AG
              527  CTCAG               
DpnII          88  GATC                 ^GATC
              248  GATC                
DraII         178  GGGTCCT              [GA]G^G[ACGT]CC[CT]
              276  GGGACCC             
DsaI           15  CCATGG               C^C[GA][CT]GG
               34  CCATGG              
EcoO109I      178  GGGTCCT              [GA]G^G[ACGT]CC[CT]
              276  GGGACCC             
EcoT14I        15  CCATGG               C^C[AT][AT]GG
               34  CCATGG              
Fnu4HI        101  GCTGC                GC^[ACGT]GC
              359  GCGGC               
ItaI          101  GCTGC                GC^[ACGT]GC
              359  GCGGC               
MboI           88  GATC                 ^GATC
              248  GATC                
MboII           7  TCTTC                GAAGA(8/7)
              112  TCTTC               
NciI           91  CCGGG                CC^[GC]GG
              175  CCGGG               
NcoI           15  CCATGG               C^CATGG
               34  CCATGG              
NdeII          88  GATC                 ^GATC
              248  GATC                
PpuMI         178  GGGTCCT              [GA]G^G[AT]CC[CT]
              276  GGGACCC             
Sau3AI         88  GATC                 ^GATC
              248  GATC                
StyI           15  CCATGG               C^C[AT][AT]GG
               34  CCATGG              
TaqI          259  TCGA                 T^CGA
              393  TCGA                
Tsp45I        207  GTCAC                ^GT[GC]AC
              456  GTCAC               
TthHB8I       259  TCGA                 T^CGA
              393  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   22 enzymes) #
#########################################

AluI        BsaJI       BsiYI       BslI        BsmAI       
BssKI       BstNI       Cac8I       Cfr13I      EcoRII      
HaeIII      HapII       HpaII       MaeIII      MnlI        
MspI        MvaI        MwoI        NlaIII      NlaIV       
Sau96I      ScrFI