################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HhaI HinP1I HindIII HpaI HphI KasI KpnI KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp509I Tth111I Van91I XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AciI 359 GCGG CCGC(-3/-1) AcyI 421 GGCGTC G[GA]^CG[CT]C AlwI 88 GATCC GGATC(4/5) AvaI 258 CTCGAG C^[CT]CG[GA]G BbvI 101 GCTGC GCAGC(8/12) BsaHI 421 GGCGTC G[GA]^CG[CT]C BsiEI 172 CGGCCG CG[GA][CT]^CG BsmFI 276 GGGAC GGGAC(10/14) BsmI 73 GAATGC GAATGC(1/-1) BsoBI 258 CTCGAG C^[CT]CG[GA]G BsrDI 293 GCAATG GCAATG(2/0) BsrFI 170 GCCGGC [GA]^CCGG[CT] BsrI 51 ACTGG ACTGG(1/-1) BstAPI 161 GCAGACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 170 GCCGGC [GA]^CCGG[CT] EaeI 172 CGGCCG [CT]^GGCC[GA] EagI 172 CGGCCG C^GGCCG EarI 111 CTCTTC CTCTTC(1/4) EclXI 172 CGGCCG C^GGCCG Eco52I 172 CGGCCG C^GGCCG HgaI 422 GCGTC GACGC(5/10) Hin1I 421 GGCGTC G[GA]^CG[CT]C HincII 47 GTCAAC GT[CT]^[GA]AC HindII 47 GTCAAC GT[CT]^[GA]AC HinfI 477 GATTC G^A[ACGT]TC Ksp632I 111 CTCTTC CTCTTC(1/4) MaeII 386 ACGT A^CGT MseI 518 TTAA T^TAA MspA1I 99 CAGCTG C[AC]G^C[GT]G NaeI 170 GCCGGC GCC^GGC NgoMIV 170 GCCGGC G^CCGGC PaeR7I 258 CTCGAG C^TCGAG PvuII 99 CAGCTG CAG^CTG SfaNI 23 GATGC GCATC(5/9) SmlI 258 CTCGAG C^T[CT][GA]AG TfiI 477 GATTC G^A[AT]TC Tru9I 518 TTAA T^TAA TseI 101 GCTGC G^C[AT]GC TspRI 166 CAGTG CA[GC]TG[ACGT][ACGT]^ XhoI 258 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AvaII 179 GGTCC G^G[AT]CC 277 GGACC BanII 215 GGGCTC G[GA]GC[CT]^C 255 GGGCTC BcnI 91 CCGGG CC^[GC]GG 175 CCGGG BmyI 215 GGGCTC G[AGT]GC[ACT]^C 255 GGGCTC BseRI 131 CTCCTC GAGGAG(10/8) 486 CTCCTC BsmBI 378 GAGACG CGTCTC(1/5) 383 GAGACG Bsp1286I 215 GGGCTC G[AGT]GC[ACT]^C 255 GGGCTC DdeI 443 CTGAG C^T[ACGT]AG 527 CTCAG DpnII 88 GATC ^GATC 248 GATC DraII 178 GGGTCCT [GA]G^G[ACGT]CC[CT] 276 GGGACCC DsaI 15 CCATGG C^C[GA][CT]GG 34 CCATGG EcoO109I 178 GGGTCCT [GA]G^G[ACGT]CC[CT] 276 GGGACCC EcoT14I 15 CCATGG C^C[AT][AT]GG 34 CCATGG Fnu4HI 101 GCTGC GC^[ACGT]GC 359 GCGGC ItaI 101 GCTGC GC^[ACGT]GC 359 GCGGC MboI 88 GATC ^GATC 248 GATC MboII 7 TCTTC GAAGA(8/7) 112 TCTTC NciI 91 CCGGG CC^[GC]GG 175 CCGGG NcoI 15 CCATGG C^CATGG 34 CCATGG NdeII 88 GATC ^GATC 248 GATC PpuMI 178 GGGTCCT [GA]G^G[AT]CC[CT] 276 GGGACCC Sau3AI 88 GATC ^GATC 248 GATC StyI 15 CCATGG C^C[AT][AT]GG 34 CCATGG TaqI 259 TCGA T^CGA 393 TCGA Tsp45I 207 GTCAC ^GT[GC]AC 456 GTCAC TthHB8I 259 TCGA T^CGA 393 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AluI BsaJI BsiYI BslI BsmAI BssKI BstNI Cac8I Cfr13I EcoRII HaeIII HapII HpaII MaeIII MnlI MspI MvaI MwoI NlaIII NlaIV Sau96I ScrFI