##################################
# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiHKAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FokI FseI
FspI HaeII HhaI HinP1I HindIII
HpaI HphI KasI KpnI KspI
MaeI MamI MfeI MflI MluI
MluNI MroI MscI MslI MunI
MvnI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp509I Tth111I Van91I XbaI
XcmI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AciI 359 GCGG CCGC(-3/-1)
AcyI 421 GGCGTC G[GA]^CG[CT]C
AlwI 88 GATCC GGATC(4/5)
AvaI 258 CTCGAG C^[CT]CG[GA]G
BbvI 101 GCTGC GCAGC(8/12)
BsaHI 421 GGCGTC G[GA]^CG[CT]C
BsiEI 172 CGGCCG CG[GA][CT]^CG
BsmFI 276 GGGAC GGGAC(10/14)
BsmI 73 GAATGC GAATGC(1/-1)
BsoBI 258 CTCGAG C^[CT]CG[GA]G
BsrDI 293 GCAATG GCAATG(2/0)
BsrFI 170 GCCGGC [GA]^CCGG[CT]
BsrI 51 ACTGG ACTGG(1/-1)
BstAPI 161 GCAGACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 170 GCCGGC [GA]^CCGG[CT]
EaeI 172 CGGCCG [CT]^GGCC[GA]
EagI 172 CGGCCG C^GGCCG
EarI 111 CTCTTC CTCTTC(1/4)
EclXI 172 CGGCCG C^GGCCG
Eco52I 172 CGGCCG C^GGCCG
HgaI 422 GCGTC GACGC(5/10)
Hin1I 421 GGCGTC G[GA]^CG[CT]C
HincII 47 GTCAAC GT[CT]^[GA]AC
HindII 47 GTCAAC GT[CT]^[GA]AC
HinfI 477 GATTC G^A[ACGT]TC
Ksp632I 111 CTCTTC CTCTTC(1/4)
MaeII 386 ACGT A^CGT
MseI 518 TTAA T^TAA
MspA1I 99 CAGCTG C[AC]G^C[GT]G
NaeI 170 GCCGGC GCC^GGC
NgoMIV 170 GCCGGC G^CCGGC
PaeR7I 258 CTCGAG C^TCGAG
PvuII 99 CAGCTG CAG^CTG
SfaNI 23 GATGC GCATC(5/9)
SmlI 258 CTCGAG C^T[CT][GA]AG
TfiI 477 GATTC G^A[AT]TC
Tru9I 518 TTAA T^TAA
TseI 101 GCTGC G^C[AT]GC
TspRI 166 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 258 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AvaII 179 GGTCC G^G[AT]CC
277 GGACC
BanII 215 GGGCTC G[GA]GC[CT]^C
255 GGGCTC
BcnI 91 CCGGG CC^[GC]GG
175 CCGGG
BmyI 215 GGGCTC G[AGT]GC[ACT]^C
255 GGGCTC
BseRI 131 CTCCTC GAGGAG(10/8)
486 CTCCTC
BsmBI 378 GAGACG CGTCTC(1/5)
383 GAGACG
Bsp1286I 215 GGGCTC G[AGT]GC[ACT]^C
255 GGGCTC
DdeI 443 CTGAG C^T[ACGT]AG
527 CTCAG
DpnII 88 GATC ^GATC
248 GATC
DraII 178 GGGTCCT [GA]G^G[ACGT]CC[CT]
276 GGGACCC
DsaI 15 CCATGG C^C[GA][CT]GG
34 CCATGG
EcoO109I 178 GGGTCCT [GA]G^G[ACGT]CC[CT]
276 GGGACCC
EcoT14I 15 CCATGG C^C[AT][AT]GG
34 CCATGG
Fnu4HI 101 GCTGC GC^[ACGT]GC
359 GCGGC
ItaI 101 GCTGC GC^[ACGT]GC
359 GCGGC
MboI 88 GATC ^GATC
248 GATC
MboII 7 TCTTC GAAGA(8/7)
112 TCTTC
NciI 91 CCGGG CC^[GC]GG
175 CCGGG
NcoI 15 CCATGG C^CATGG
34 CCATGG
NdeII 88 GATC ^GATC
248 GATC
PpuMI 178 GGGTCCT [GA]G^G[AT]CC[CT]
276 GGGACCC
Sau3AI 88 GATC ^GATC
248 GATC
StyI 15 CCATGG C^C[AT][AT]GG
34 CCATGG
TaqI 259 TCGA T^CGA
393 TCGA
Tsp45I 207 GTCAC ^GT[GC]AC
456 GTCAC
TthHB8I 259 TCGA T^CGA
393 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AluI BsaJI BsiYI BslI BsmAI
BssKI BstNI Cac8I Cfr13I EcoRII
HaeIII HapII HpaII MaeIII MnlI
MspI MvaI MwoI NlaIII NlaIV
Sau96I ScrFI