##################################
# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII Acc65I AccIII AciI AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr13I ClaI CpoI
DraII DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HindIII HinfI
HphI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MroI
MslI MspA1I MunI NarI NciI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmlI
PpuMI PshAI Psp1406I PvuII RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tsp45I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 155 GTCTAC GT^[AC][GT]AC
AccII 199 CGCG CG^CG
AflIII 41 ACACGT A^C[GA][CT]GT
AseI 410 ATTAAT AT^TAAT
AsnI 410 ATTAAT AT^TAAT
BamHI 318 GGATCC G^GATCC
BglII 21 AGATCT A^GATCT
BpmI 305 CTGGAG CTGGAG(16/14)
BsaJI 176 CCAAGG C^C[ACGT][ACGT]GG
BsiEI 160 CGATCG CG[GA][CT]^CG
BsrFI 328 GCCGGC [GA]^CCGG[CT]
BssKI 244 CCAGG ^CC[ACGT]GG
BstAPI 386 GCACTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 180 GGATG GGATG(2/0)
BstNI 244 CCAGG CC^[AT]GG
BstUI 199 CGCG CG^CG
Cac8I 328 GCCGGC GC[ACGT]^[ACGT]GC
Cfr10I 328 GCCGGC [GA]^CCGG[CT]
DraI 218 TTTAAA TTT^AAA
EcoNI 499 CCTCTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 244 CCAGG ^CC[AT]GG
EcoT14I 176 CCAAGG C^C[AT][AT]GG
FokI 180 GGATG GGATG(9/13)
HapII 329 CCGG C^CGG
HincII 478 GTTAAC GT[CT]^[GA]AC
HindII 478 GTTAAC GT[CT]^[GA]AC
HpaI 478 GTTAAC GTT^AAC
HpaII 329 CCGG C^CGG
MaeII 43 ACGT A^CGT
MaeIII 288 GTAAC ^GT[ACGT]AC
MspI 329 CCGG C^CGG
MvaI 244 CCAGG CC^[AT]GG
MvnI 199 CGCG CG^CG
NaeI 328 GCCGGC GCC^GGC
NgoMIV 328 GCCGGC G^CCGGC
NlaIV 318 GGATCC GG[ACGT]^[ACGT]CC
NspI 391 ACATGC [GA]CATG^[CT]
PmeI 217 GTTTAAAC GTTT^AAAC
PshBI 410 ATTAAT AT^TAAT
PstI 396 CTGCAG CTGCA^G
PvuI 160 CGATCG CGAT^CG
ScrFI 244 CCAGG CC^[ACGT]GG
SfaNI 325 GATGC GCATC(5/9)
SfcI 396 CTGCAG C^T[GA][CT]AG
StyI 176 CCAAGG C^C[AT][AT]GG
TaqI 70 TCGA T^CGA
TthHB8I 70 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 313 GTAC GT^AC
511 GTAC
BalI 357 TGGCCA TGG^CCA
435 TGGCCA
BseRI 275 GAGGAG GAGGAG(10/8)
278 GAGGAG
BsgI 384 CTGCAC GTGCAG(16/14)
89 GTGCAG
BsmI 192 GCATTC GAATGC(1/-1)
351 GCATTC
BsrI 223 ACTGG ACTGG(1/-1)
304 ACTGG
CfoI 200 GCGC GCG^C
233 GCGC
DdeI 203 CTAAG C^T[ACGT]AG
458 CTAAG
EaeI 357 TGGCCA [CT]^GGCC[GA]
435 TGGCCA
HhaI 200 GCGC GCG^C
233 GCGC
HinP1I 200 GCGC G^CGC
233 GCGC
MboII 16 GAAGA GAAGA(8/7)
19 GAAGA
MluNI 357 TGGCCA TGG^CCA
435 TGGCCA
MscI 357 TGGCCA TGG^CCA
435 TGGCCA
NlaIII 292 CATG CATG^
392 CATG
RsaI 313 GTAC GT^AC
511 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AluI AlwI BbvI BsiYI BslI
BstYI DpnII Fnu4HI HaeIII ItaI
MboI MflI MnlI MseI MwoI
NdeII Sau3AI Tru9I TseI TspRI
XhoII