################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccIII AciI AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr13I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HindIII HinfI HphI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MslI MspA1I MunI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmlI PpuMI PshAI Psp1406I PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 155 GTCTAC GT^[AC][GT]AC AccII 199 CGCG CG^CG AflIII 41 ACACGT A^C[GA][CT]GT AseI 410 ATTAAT AT^TAAT AsnI 410 ATTAAT AT^TAAT BamHI 318 GGATCC G^GATCC BglII 21 AGATCT A^GATCT BpmI 305 CTGGAG CTGGAG(16/14) BsaJI 176 CCAAGG C^C[ACGT][ACGT]GG BsiEI 160 CGATCG CG[GA][CT]^CG BsrFI 328 GCCGGC [GA]^CCGG[CT] BssKI 244 CCAGG ^CC[ACGT]GG BstAPI 386 GCACTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 180 GGATG GGATG(2/0) BstNI 244 CCAGG CC^[AT]GG BstUI 199 CGCG CG^CG Cac8I 328 GCCGGC GC[ACGT]^[ACGT]GC Cfr10I 328 GCCGGC [GA]^CCGG[CT] DraI 218 TTTAAA TTT^AAA EcoNI 499 CCTCTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 244 CCAGG ^CC[AT]GG EcoT14I 176 CCAAGG C^C[AT][AT]GG FokI 180 GGATG GGATG(9/13) HapII 329 CCGG C^CGG HincII 478 GTTAAC GT[CT]^[GA]AC HindII 478 GTTAAC GT[CT]^[GA]AC HpaI 478 GTTAAC GTT^AAC HpaII 329 CCGG C^CGG MaeII 43 ACGT A^CGT MaeIII 288 GTAAC ^GT[ACGT]AC MspI 329 CCGG C^CGG MvaI 244 CCAGG CC^[AT]GG MvnI 199 CGCG CG^CG NaeI 328 GCCGGC GCC^GGC NgoMIV 328 GCCGGC G^CCGGC NlaIV 318 GGATCC GG[ACGT]^[ACGT]CC NspI 391 ACATGC [GA]CATG^[CT] PmeI 217 GTTTAAAC GTTT^AAAC PshBI 410 ATTAAT AT^TAAT PstI 396 CTGCAG CTGCA^G PvuI 160 CGATCG CGAT^CG ScrFI 244 CCAGG CC^[ACGT]GG SfaNI 325 GATGC GCATC(5/9) SfcI 396 CTGCAG C^T[GA][CT]AG StyI 176 CCAAGG C^C[AT][AT]GG TaqI 70 TCGA T^CGA TthHB8I 70 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AfaI 313 GTAC GT^AC 511 GTAC BalI 357 TGGCCA TGG^CCA 435 TGGCCA BseRI 275 GAGGAG GAGGAG(10/8) 278 GAGGAG BsgI 384 CTGCAC GTGCAG(16/14) 89 GTGCAG BsmI 192 GCATTC GAATGC(1/-1) 351 GCATTC BsrI 223 ACTGG ACTGG(1/-1) 304 ACTGG CfoI 200 GCGC GCG^C 233 GCGC DdeI 203 CTAAG C^T[ACGT]AG 458 CTAAG EaeI 357 TGGCCA [CT]^GGCC[GA] 435 TGGCCA HhaI 200 GCGC GCG^C 233 GCGC HinP1I 200 GCGC G^CGC 233 GCGC MboII 16 GAAGA GAAGA(8/7) 19 GAAGA MluNI 357 TGGCCA TGG^CCA 435 TGGCCA MscI 357 TGGCCA TGG^CCA 435 TGGCCA NlaIII 292 CATG CATG^ 392 CATG RsaI 313 GTAC GT^AC 511 GTAC ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AluI AlwI BbvI BsiYI BslI BstYI DpnII Fnu4HI HaeIII ItaI MboI MflI MnlI MseI MwoI NdeII Sau3AI Tru9I TseI TspRI XhoII