##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AclI AcsI AcyI
AflII AgeI AhdI AlwNI Aor51HI
ApoI AscI AseI AsnI Asp718I
AspEI AviII AvrII BalI BamHI
BbeI BbrPI BcgI BciVI BclI
BfrI BlnI BsaAI BsaBI BsaHI
BsiWI BsmBI BspDI BspHI BsrBI
BssHII BssSI BstBI BstEII BstPI
BstXI ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MscI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccIII 494 TCCGGA T^CCGGA
Alw44I 684 GTGCAC G^TGCAC
AocI 518 CCTGAGG CC^T[ACGT]AGG
ApaI 1441 GGGCCC GGGCC^C
ApaLI 684 GTGCAC G^TGCAC
AspI 1349 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 968 GGTGCC G^G[CT][GA]CC
BbvCI 916 CCTCAGC CCTCAGC(-5/-2)
BglI 39 GCCCGTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 609 AGATCT A^GATCT
BlpI 943 GCTGAGC GC^T[ACGT]AGC
BpmI 1091 CTCCAG CTGGAG(16/14)
Bpu1102I 943 GCTGAGC GC^T[ACGT]AGC
BsaWI 494 TCCGGA [AT]^CCGG[AT]
BseAI 494 TCCGGA T^CCGGA
BsgI 989 CTGCAC GTGCAG(16/14)
BsiEI 169 CGACCG CG[GA][CT]^CG
Bsp1407I 1570 TGTACA T^GTACA
BspEI 494 TCCGGA T^CCGGA
BsrDI 342 GCAATG GCAATG(2/0)
BsrGI 1570 TGTACA T^GTACA
Bst1107I 1476 GTATAC GTA^TAC
BstAPI 1525 GCATGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 609 AGATCT [GA]^GATC[CT]
BstZ17I 1476 GTATAC GTA^TAC
Bsu36I 518 CCTGAGG CC^T[ACGT]AGG
CelII 943 GCTGAGC GC^T[ACGT]AGC
DsaI 1422 CCGTGG C^C[GA][CT]GG
EaeI 759 CGGCCA [CT]^GGCC[GA]
Eco81I 518 CCTGAGG CC^T[ACGT]AGG
EcoNI 841 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 541 CCAAGG C^C[AT][AT]GG
FauI 229 CCCGC CCCGC(4/6)
MaeIII 113 GTCAC ^GT[ACGT]AC
MflI 609 AGATCT [GA]^GATC[CT]
MroI 494 TCCGGA T^CCGGA
MspA1I 774 CAGCTG C[AC]G^C[GT]G
NaeI 757 GCCGGC GCC^GGC
NgoMIV 757 GCCGGC G^CCGGC
PaeR7I 1056 CTCGAG C^TCGAG
PflFI 1349 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1074 AGGACCC [GA]G^G[AT]CC[CT]
PstI 587 CTGCAG CTGCA^G
PvuII 774 CAGCTG CAG^CTG
SapI 1463 GAAGAGC GCTCTTC(1/4)
ScaI 624 AGTACT AGT^ACT
SspBI 1570 TGTACA T^GTACA
StyI 541 CCAAGG C^C[AT][AT]GG
Tsp45I 113 GTCAC ^GT[GC]AC
Tth111I 1349 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1056 CTCGAG C^TCGAG
XhoII 609 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 215 GTCTAC GT^[AC][GT]AC
1476 GTATAC
AlwI 277 GGATC GGATC(4/5)
1305 GGATC
Asp700I 470 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1629 GAAACGGTTC
BanII 89 GAGCCC G[GA]GC[CT]^C
1441 GGGCCC
BbsI 1118 GAAGAC GAAGAC(2/6)
1202 GTCTTC
BcnI 797 CCCGG CC^[GC]GG
1039 CCCGG
BpuAI 1118 GAAGAC GAAGAC(2/6)
1202 GTCTTC
BsaI 17 GGTCTC GGTCTC(1/5)
703 GGTCTC
BseRI 442 CTCCTC GAGGAG(10/8)
937 CTCCTC
BsmI 769 GAATGC GAATGC(1/-1)
823 GAATGC
BspMI 712 ACCTGC ACCTGC(4/8)
987 ACCTGC
BsrFI 109 GCCGGT [GA]^CCGG[CT]
757 GCCGGC
Cfr10I 109 GCCGGT [GA]^CCGG[CT]
757 GCCGGC
DraI 1763 TTTAAA TTT^AAA
1783 TTTAAA
DrdI 24 GACGGCTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1301 GACTGGATCGTC
HaeII 141 AGCGCC [GA]GCGC^[CT]
225 AGCGCC
HphI 124 GGTGA GGTGA(8/7)
280 TCACC
MslI 958 CATGGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1583 CATAGACGTG
NciI 797 CCCGG CC^[GC]GG
1039 CCCGG
PflMI 541 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1254 CCAACTGATGG
SfcI 587 CTGCAG C^T[GA][CT]AG
975 CTGTAG
StuI 515 AGGCCT AGG^CCT
544 AGGCCT
Van91I 541 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1254 CCAACTGATGG
XmnI 470 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1629 GAAACGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AspHI AvaI AvaII BbvI BfaI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspLU11I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MaeI MaeII MboI MboII
MnlI MseI MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I