##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvrII BamHI
BbeI BbrPI BcgI BciVI BclI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI Hin1I HindIII HpaI KasI
KpnI MamI MfeI MluI MroI
MseI MunI NarI NdeI NheI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
SwaI TfiI Tru9I Tth111I XbaI
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AfaI 252 GTAC GT^AC
Asp700I 928 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 823 TGCGCA TGC^GCA
BalI 482 TGGCCA TGG^CCA
BanI 1057 GGTGCC G^G[CT][GA]CC
BanII 15 GAGCCC G[GA]GC[CT]^C
BbsI 766 GAAGAC GAAGAC(2/6)
BbvCI 1120 GCTGAGG CCTCAGC(-5/-2)
BglI 496 GCCGTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 423 AGATCT A^GATCT
BpuAI 766 GAAGAC GAAGAC(2/6)
BsaI 1165 GGTCTC GGTCTC(1/5)
BspLU11I 131 ACATGT A^CATGT
BstYI 423 AGATCT [GA]^GATC[CT]
EarI 520 GAAGAG CTCTTC(1/4)
FspI 823 TGCGCA TGC^GCA
HincII 748 GTTGAC GT[CT]^[GA]AC
HindII 748 GTTGAC GT[CT]^[GA]AC
Ksp632I 520 GAAGAG CTCTTC(1/4)
MaeII 365 ACGT A^CGT
MaeIII 1130 GTGAC ^GT[ACGT]AC
MflI 423 AGATCT [GA]^GATC[CT]
MluNI 482 TGGCCA TGG^CCA
MscI 482 TGGCCA TGG^CCA
MslI 1068 CATATTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NotI 300 GCGGCCGC GC^GGCCGC
NspI 131 ACATGT [GA]CATG^[CT]
PaeR7I 12 CTCGAG C^TCGAG
PflMI 1048 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 965 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 697 CAGCTG CAG^CTG
RsaI 252 GTAC GT^AC
SbfI 681 CCTGCAGG CCTGCA^GG
Sse8387I 681 CCTGCAGG CCTGCA^GG
StuI 1181 AGGCCT AGG^CCT
TaqI 13 TCGA T^CGA
Tsp45I 1130 GTGAC ^GT[GC]AC
Tsp509I 1215 AATT ^AATT
TthHB8I 13 TCGA T^CGA
Van91I 1048 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 12 CTCGAG C^TCGAG
XhoII 423 AGATCT [GA]^GATC[CT]
XmnI 928 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 131 ACATGT A^C[GA][CT]GT
365 ACGTGT
AocI 895 CCTAAGG CC^T[ACGT]AGG
1017 CCTGAGG
AvaI 12 CTCGAG C^[CT]CG[GA]G
221 CCCGAG
AvaII 540 GGACC G^G[AT]CC
966 GGACC
BfaI 421 CTAG C^TAG
865 CTAG
BsmBI 1170 CGTCTC CGTCTC(1/5)
523 GAGACG
BsoBI 12 CTCGAG C^[CT]CG[GA]G
221 CCCGAG
BsrI 186 ACTGG ACTGG(1/-1)
310 CCAGT
BssHII 150 GCGCGC G^CGCGC
408 GCGCGC
BstF5I 27 GGATG GGATG(2/0)
945 GGATG
BstXI 485 CCACGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
975 CCAGGCCCATGG
Bsu36I 895 CCTAAGG CC^T[ACGT]AGG
1017 CCTGAGG
DpnII 424 GATC ^GATC
1245 GATC
Eco81I 895 CCTAAGG CC^T[ACGT]AGG
1017 CCTGAGG
EcoNI 681 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
969 CCTGTGCCAGG
FokI 27 GGATG GGATG(9/13)
945 GGATG
HaeII 170 AGCGCC [GA]GCGC^[CT]
590 GGCGCT
HphI 343 GGTGA GGTGA(8/7)
1129 GGTGA
KspI 357 CCGCGG CCGC^GG
536 CCGCGG
MaeI 421 CTAG C^TAG
865 CTAG
MboI 424 GATC ^GATC
1245 GATC
NaeI 257 GCCGGC GCC^GGC
261 GCCGGC
NcoI 113 CCATGG C^CATGG
981 CCATGG
NdeII 424 GATC ^GATC
1245 GATC
NgoMIV 257 GCCGGC G^CCGGC
261 GCCGGC
SacII 357 CCGCGG CCGC^GG
536 CCGCGG
Sau3AI 424 GATC ^GATC
1245 GATC
SfaNI 28 GATGC GCATC(5/9)
58 GATGC
SmlI 12 CTCGAG C^T[CT][GA]AG
1157 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AluI AspHI BbvI
BcnI BmyI BpmI BsaJI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DraII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PleI PstI Sau96I ScrFI SfcI
StyI TseI TspRI