##################################
# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspHI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspMI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI MamI
MfeI MluI MroI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 1135 GTCTAC GT^[AC][GT]AC
AccII 577 CGCG CG^CG
AflII 955 CTTAAG C^TTAAG
AflIII 1355 ACATGT A^C[GA][CT]GT
AhdI 219 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 120 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 798 CCTCAGG CC^T[ACGT]AGG
ApaI 1233 GGGCCC GGGCC^C
Asp700I 359 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 219 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 508 CCCGAG C^[CT]CG[GA]G
BcnI 632 CCGGG CC^[GC]GG
BfrI 955 CTTAAG C^TTAAG
BglII 667 AGATCT A^GATCT
BpmI 1141 CTCCAG CTGGAG(16/14)
BsgI 261 CTGCAC GTGCAG(16/14)
BsoBI 508 CCCGAG C^[CT]CG[GA]G
BspLU11I 1355 ACATGT A^CATGT
BsrBI 726 GAGCGG CCGCTC(-3/-3)
BsrDI 1014 GCAATG GCAATG(2/0)
BstAPI 413 GCAGGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 577 CGCG CG^CG
Bsu36I 798 CCTCAGG CC^T[ACGT]AGG
DrdI 935 GACTTAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 576 CCGCGG C^C[GA][CT]GG
Eam1105I 219 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 798 CCTCAGG CC^T[ACGT]AGG
EcoRV 209 GATATC GAT^ATC
EcoT14I 445 CCAAGG C^C[AT][AT]GG
EcoT22I 660 ATGCAT ATGCA^T
KspI 576 CCGCGG CCGC^GG
MaeII 521 ACGT A^CGT
MvnI 577 CGCG CG^CG
NciI 632 CCGGG CC^[GC]GG
NsiI 660 ATGCAT ATGCA^T
NspI 1355 ACATGT [GA]CATG^[CT]
PflMI 25 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 123 CAGCTG CAG^CTG
SacII 576 CCGCGG CCGC^GG
SmlI 955 CTTAAG C^T[CT][GA]AG
StuI 415 AGGCCT AGG^CCT
StyI 445 CCAAGG C^C[AT][AT]GG
Van91I 25 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 289 CCATGAAACGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 359 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 1407 GAATTT [GA]^AATT[CT]
1431 GAATTT
AfaI 458 GTAC GT^AC
917 GTAC
AlwI 332 GATCC GGATC(4/5)
629 GATCC
ApoI 1407 GAATTT [GA]^AATT[CT]
1431 GAATTT
BbsI 473 GAAGAC GAAGAC(2/6)
534 GAAGAC
BglI 177 GCCGACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
974 GCCTCACAGGC
BpuAI 473 GAAGAC GAAGAC(2/6)
534 GAAGAC
BstXI 76 CCAAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
887 CCAGGCAGTTGG
BstYI 331 AGATCC [GA]^GATC[CT]
667 AGATCT
DraIII 235 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
264 CACATGGTG
EarI 129 GAAGAG CTCTTC(1/4)
513 GAAGAG
HapII 1 CCGG C^CGG
632 CCGG
HpaII 1 CCGG C^CGG
632 CCGG
Ksp632I 129 GAAGAG CTCTTC(1/4)
513 GAAGAG
MflI 331 AGATCC [GA]^GATC[CT]
667 AGATCT
MspA1I 123 CAGCTG C[AC]G^C[GT]G
576 CCGCGG
MspI 1 CCGG C^CGG
632 CCGG
PpuMI 217 AGGACCT [GA]G^G[AT]CC[CT]
1147 AGGACCT
RsaI 458 GTAC GT^AC
917 GTAC
SapI 129 GAAGAGC GCTCTTC(1/4)
513 GAAGAGC
TfiI 35 GATTC G^A[AT]TC
866 GATTC
Tsp45I 270 GTGAC ^GT[GC]AC
830 GTGAC
Tsp509I 1408 AATT ^AATT
1432 AATT
XhoII 331 AGATCC [GA]^GATC[CT]
667 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AvaII BalI BanII
BbvI BfaI BmyI BsaI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI Tru9I TseI TspRI