################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspHI AspI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI EagI EclXI Eco47III Eco52I EcoO65I EcoRI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 1135 GTCTAC GT^[AC][GT]AC AccII 577 CGCG CG^CG AflII 955 CTTAAG C^TTAAG AflIII 1355 ACATGT A^C[GA][CT]GT AhdI 219 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 120 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 798 CCTCAGG CC^T[ACGT]AGG ApaI 1233 GGGCCC GGGCC^C Asp700I 359 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 219 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 508 CCCGAG C^[CT]CG[GA]G BcnI 632 CCGGG CC^[GC]GG BfrI 955 CTTAAG C^TTAAG BglII 667 AGATCT A^GATCT BpmI 1141 CTCCAG CTGGAG(16/14) BsgI 261 CTGCAC GTGCAG(16/14) BsoBI 508 CCCGAG C^[CT]CG[GA]G BspLU11I 1355 ACATGT A^CATGT BsrBI 726 GAGCGG CCGCTC(-3/-3) BsrDI 1014 GCAATG GCAATG(2/0) BstAPI 413 GCAGGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 577 CGCG CG^CG Bsu36I 798 CCTCAGG CC^T[ACGT]AGG DrdI 935 GACTTAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 576 CCGCGG C^C[GA][CT]GG Eam1105I 219 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 798 CCTCAGG CC^T[ACGT]AGG EcoRV 209 GATATC GAT^ATC EcoT14I 445 CCAAGG C^C[AT][AT]GG EcoT22I 660 ATGCAT ATGCA^T KspI 576 CCGCGG CCGC^GG MaeII 521 ACGT A^CGT MvnI 577 CGCG CG^CG NciI 632 CCGGG CC^[GC]GG NsiI 660 ATGCAT ATGCA^T NspI 1355 ACATGT [GA]CATG^[CT] PflMI 25 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 123 CAGCTG CAG^CTG SacII 576 CCGCGG CCGC^GG SmlI 955 CTTAAG C^T[CT][GA]AG StuI 415 AGGCCT AGG^CCT StyI 445 CCAAGG C^C[AT][AT]GG Van91I 25 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 289 CCATGAAACGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 359 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 1407 GAATTT [GA]^AATT[CT] 1431 GAATTT AfaI 458 GTAC GT^AC 917 GTAC AlwI 332 GATCC GGATC(4/5) 629 GATCC ApoI 1407 GAATTT [GA]^AATT[CT] 1431 GAATTT BbsI 473 GAAGAC GAAGAC(2/6) 534 GAAGAC BglI 177 GCCGACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 974 GCCTCACAGGC BpuAI 473 GAAGAC GAAGAC(2/6) 534 GAAGAC BstXI 76 CCAAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 887 CCAGGCAGTTGG BstYI 331 AGATCC [GA]^GATC[CT] 667 AGATCT DraIII 235 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG 264 CACATGGTG EarI 129 GAAGAG CTCTTC(1/4) 513 GAAGAG HapII 1 CCGG C^CGG 632 CCGG HpaII 1 CCGG C^CGG 632 CCGG Ksp632I 129 GAAGAG CTCTTC(1/4) 513 GAAGAG MflI 331 AGATCC [GA]^GATC[CT] 667 AGATCT MspA1I 123 CAGCTG C[AC]G^C[GT]G 576 CCGCGG MspI 1 CCGG C^CGG 632 CCGG PpuMI 217 AGGACCT [GA]G^G[AT]CC[CT] 1147 AGGACCT RsaI 458 GTAC GT^AC 917 GTAC SapI 129 GAAGAGC GCTCTTC(1/4) 513 GAAGAGC TfiI 35 GATTC G^A[AT]TC 866 GATTC Tsp45I 270 GTGAC ^GT[GC]AC 830 GTGAC Tsp509I 1408 AATT ^AATT 1432 AATT XhoII 331 AGATCC [GA]^GATC[CT] 667 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AluI AvaII BalI BanII BbvI BfaI BmyI BsaI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MluNI MnlI MscI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PleI PstI Sau3AI Sau96I ScrFI SfaNI SfcI Tru9I TseI TspRI