##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
Acc65I AccIII AclI AcsI AfaI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BbrPI BbvCI
BciVI BclI BlnI BpmI BsaBI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII BssSI BstEII BstPI Bsu36I
ClaI CpoI DraIII DrdI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I FbaI FseI
FspI HindIII KpnI Ksp632I MamI
MfeI MluI MluNI MroI MscI
MslI MunI NcoI NheI NotI
NruI PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SalI SapI ScaI SexAI SfiI
SgrAI SnaBI SpeI SphI SspBI
StyI SwaI Tth111I XbaI XcmI
###################################
# ONE CUT ENZYMES ( 65 enzymes) #
###################################
AatII 335 GACGTC GACGT^C
AflII 1062 CTTAAG C^TTAAG
AlwI 953 GGATC GGATC(4/5)
AlwNI 691 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 362 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 46 GGTCC G^G[AT]CC
BanI 176 GGCGCC G^G[CT][GA]CC
BbeI 176 GGCGCC GGCGC^C
BcgI 130 GCACCGGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 37 CTAG C^TAG
BfrI 1062 CTTAAG C^TTAAG
BglI 179 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1361 TACGTG [CT]AC^GT[GA]
BsaI 1057 GAGACC GGTCTC(1/5)
BsgI 852 GTGCAG GTGCAG(16/14)
BsiEI 253 CGGCCG CG[GA][CT]^CG
BspMI 465 GCAGGT ACCTGC(4/8)
BsrBI 171 GAGCGG CCGCTC(-3/-3)
BsrFI 255 GCCGGC [GA]^CCGG[CT]
Bst1107I 1265 GTATAC GTA^TAC
BstAPI 1380 GCAGGGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 512 TTCGAA TT^CGAA
BstNI 791 CCAGG CC^[AT]GG
BstXI 1092 CCAGAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1265 GTATAC GTA^TAC
Cfr10I 255 GCCGGC [GA]^CCGG[CT]
DraI 1219 TTTAAA TTT^AAA
DsaI 111 CCGCGG C^C[GA][CT]GG
EagI 253 CGGCCG C^GGCCG
EclXI 253 CGGCCG C^GGCCG
Eco52I 253 CGGCCG C^GGCCG
EcoRII 791 CCAGG ^CC[AT]GG
EcoT22I 1176 ATGCAT ATGCA^T
HincII 1118 GTTAAC GT[CT]^[GA]AC
HindII 1118 GTTAAC GT[CT]^[GA]AC
HpaI 1118 GTTAAC GTT^AAC
HphI 385 TCACC GGTGA(8/7)
KasI 176 GGCGCC G^GCGCC
KspI 111 CCGCGG CCGC^GG
MaeI 37 CTAG C^TAG
MvaI 791 CCAGG CC^[AT]GG
NaeI 255 GCCGGC GCC^GGC
NarI 176 GGCGCC GG^CGCC
NdeI 452 CATATG CA^TATG
NgoMIV 255 GCCGGC G^CCGGC
NsiI 1176 ATGCAT ATGCA^T
NspI 1178 GCATGT [GA]CATG^[CT]
NspV 512 TTCGAA TT^CGAA
PaeR7I 821 CTCGAG C^TCGAG
PflMI 482 CCACGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 45 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 750 GAGCTC GAGCT^C
SacII 111 CCGCGG CCGC^GG
SbfI 392 CCTGCAGG CCTGCA^GG
SfaNI 399 GCATC GCATC(5/9)
SfoI 176 GGCGCC GGC^GCC
SfuI 512 TTCGAA TT^CGAA
SmaI 163 CCCGGG CCC^GGG
Sse8387I 392 CCTGCAGG CCTGCA^GG
StuI 102 AGGCCT AGG^CCT
Van91I 482 CCACGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 821 CTCGAG C^TCGAG
XmaCI 163 CCCGGG C^CCGGG
XmaI 163 CCCGGG C^CCGGG
XmnI 362 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 1265 GTATAC GT^[AC][GT]AC
1405 GTCTAC
AspHI 128 GAGCAC G[AT]GC[AT]^C
750 GAGCTC
BbsI 332 GAAGAC GAAGAC(2/6)
972 GAAGAC
BglII 610 AGATCT A^GATCT
1227 AGATCT
BlpI 917 GCTCAGC GC^T[ACGT]AGC
1442 GCTGAGC
Bpu1102I 917 GCTCAGC GC^T[ACGT]AGC
1442 GCTGAGC
BpuAI 332 GAAGAC GAAGAC(2/6)
972 GAAGAC
BsiHKAI 128 GAGCAC G[AT]GC[AT]^C
750 GAGCTC
BsmBI 662 CGTCTC CGTCTC(1/5)
2 GAGACG
BsrDI 957 CATTGC GCAATG(2/0)
814 GCAATG
BsrI 488 ACTGG ACTGG(1/-1)
574 ACTGG
BstYI 610 AGATCT [GA]^GATC[CT]
1227 AGATCT
CelII 917 GCTCAGC GC^T[ACGT]AGC
1442 GCTGAGC
EaeI 60 TGGCCG [CT]^GGCC[GA]
253 CGGCCG
HaeII 176 GGCGCC [GA]GCGC^[CT]
210 GGCGCT
HgaI 4 GACGC GACGC(5/10)
120 GACGC
MflI 610 AGATCT [GA]^GATC[CT]
1227 AGATCT
MspA1I 111 CCGCGG C[AC]G^C[GT]G
887 CAGCGG
NlaIII 969 CATG CATG^
1179 CATG
PleI 693 GAGTC GAGTC(4/5)
824 GAGTC
PstI 393 CTGCAG CTGCA^G
1052 CTGCAG
SfcI 393 CTGCAG C^T[GA][CT]AG
1052 CTGCAG
SspI 423 AATATT AAT^ATT
1478 AATATT
TspRI 487 CACTG CA[GC]TG[ACGT][ACGT]^
1050 CACTG
XhoII 610 AGATCT [GA]^GATC[CT]
1227 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AvaI BanII BbvI BcnI BmyI
BsaHI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BssKI
BstF5I BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I FauI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII ItaI
MaeII MaeIII MboI MboII MnlI
MseI MspI MvnI MwoI NciI
NdeII NlaIV Sau3AI Sau96I ScrFI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TthHB8I