##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII BamHI BanI
BbeI BcgI BciVI BclI BfrI
BglII BlpI Bpu1102I BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FbaI FseI FspI HaeII
HgaI HincII HindII HpaI KasI
KpnI KspI MfeI MflI MluI
MroI MslI MunI NarI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI ScaI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI SspBI SspI SwaI
TaqI Tth111I TthHB8I XhoI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 297 GACGTC GACGT^C
AccII 1535 CGCG CG^CG
AcyI 297 GACGTC G[GA]^CG[CT]C
AocI 215 CCTGAGG CC^T[ACGT]AGG
ApaI 1539 GGGCCC GGGCC^C
AvaI 455 CCCGAG C^[CT]CG[GA]G
AvrII 1044 CCTAGG C^CTAGG
BalI 194 TGGCCA TGG^CCA
BbrPI 738 CACGTG CAC^GTG
BbvCI 527 GCTGAGG CCTCAGC(-5/-2)
BcnI 779 CCCGG CC^[GC]GG
BlnI 1044 CCTAGG C^CTAGG
BsaBI 310 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 297 GACGTC G[GA]^CG[CT]C
BsoBI 455 CCCGAG C^[CT]CG[GA]G
BsrFI 85 GCCGGC [GA]^CCGG[CT]
BstUI 1535 CGCG CG^CG
Bsu36I 215 CCTGAGG CC^T[ACGT]AGG
CfoI 1534 GCGC GCG^C
Cfr10I 85 GCCGGC [GA]^CCGG[CT]
DraIII 134 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1206 CCATGG C^C[GA][CT]GG
EaeI 194 TGGCCA [CT]^GGCC[GA]
Eco81I 215 CCTGAGG CC^T[ACGT]AGG
EcoT22I 1249 ATGCAT ATGCA^T
HhaI 1534 GCGC GCG^C
Hin1I 297 GACGTC G[GA]^CG[CT]C
HinP1I 1534 GCGC G^CGC
HindIII 895 AAGCTT A^AGCTT
MamI 310 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 194 TGGCCA TGG^CCA
MscI 194 TGGCCA TGG^CCA
MvnI 1535 CGCG CG^CG
NaeI 85 GCCGGC GCC^GGC
NciI 779 CCCGG CC^[GC]GG
NcoI 1206 CCATGG C^CATGG
NgoMIV 85 GCCGGC G^CCGGC
NsiI 1249 ATGCAT ATGCA^T
NspI 1251 GCATGT [GA]CATG^[CT]
PmaCI 738 CACGTG CAC^GTG
PmlI 738 CACGTG CAC^GTG
PpuMI 1077 AGGACCT [GA]G^G[AT]CC[CT]
SbfI 634 CCTGCAGG CCTGCA^GG
SexAI 567 ACCTGGT A^CC[AT]GGT
SnaBI 1050 TACGTA TAC^GTA
Sse8387I 634 CCTGCAGG CCTGCA^GG
StuI 65 AGGCCT AGG^CCT
TfiI 1099 GATTC G^A[AT]TC
Tsp509I 593 AATT ^AATT
XbaI 588 TCTAGA T^CTAGA
XcmI 315 CCATCTTGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BbsI 388 GAAGAC GAAGAC(2/6)
447 GTCTTC
BglI 1193 GCCTTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1525 GCCGTGCAGGC
BpuAI 388 GAAGAC GAAGAC(2/6)
447 GTCTTC
BsaAI 738 CACGTG [CT]AC^GT[GA]
1050 TACGTA
BsrI 1004 ACTGG ACTGG(1/-1)
366 CCAGT
BstAPI 133 GCACACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
221 GCAGCAGCTGC
DpnII 422 GATC ^GATC
751 GATC
EarI 957 CTCTTC CTCTTC(1/4)
1370 GAAGAG
EcoNI 634 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1232 CCTTCAGAAGG
Ksp632I 957 CTCTTC CTCTTC(1/4)
1370 GAAGAG
MboI 422 GATC ^GATC
751 GATC
MseI 1246 TTAA T^TAA
1279 TTAA
NdeII 422 GATC ^GATC
751 GATC
Sau3AI 422 GATC ^GATC
751 GATC
SmlI 1350 CTCAAG C^T[CT][GA]AG
1614 CTTGAG
Tru9I 1246 TTAA T^TAA
1279 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BanII BbvI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DraII EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboII MnlI MspA1I MspI
MvaI MwoI NlaIII NlaIV PflMI
PleI PstI PvuII RsaI Sau96I
ScrFI SfaNI SfcI StyI TseI
Tsp45I TspRI Van91I