##################################
# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BbrPI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaWI
BseAI BseRI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstBI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HincII HindII HpaI
KpnI Ksp632I MamI MflI MluI
MroI MslI NcoI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tth111I TthHB8I XbaI XcmI XhoI
XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AcyI 18 GGCGCC G[GA]^CG[CT]C
Asp700I 1473 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 1455 GAGCCC G[GA]GC[CT]^C
BbeI 18 GGCGCC GGCGC^C
BbsI 195 GAAGAC GAAGAC(2/6)
BbvCI 147 CCTCAGC CCTCAGC(-5/-2)
BpuAI 195 GAAGAC GAAGAC(2/6)
BsaHI 18 GGCGCC G[GA]^CG[CT]C
BsaI 1197 GAGACC GGTCTC(1/5)
BsgI 160 GTGCAG GTGCAG(16/14)
BspHI 1384 TCATGA T^CATGA
BspMI 1251 GCAGGT ACCTGC(4/8)
BsrFI 64 GCCGGC [GA]^CCGG[CT]
BssSI 1202 CACGAG CACGAG(-5/-1)
BstAPI 637 GCAAAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1209 GGTTACC G^GT[ACGT]ACC
BstPI 1209 GGTTACC G^GT[ACGT]ACC
Cfr10I 64 GCCGGC [GA]^CCGG[CT]
DpnII 700 GATC ^GATC
DraI 1256 TTTAAA TTT^AAA
DraII 1025 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 33 CCGCGG C^C[GA][CT]GG
EcoNI 726 CCTGCTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1025 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1209 GGTTACC G^GT[ACGT]ACC
Hin1I 18 GGCGCC G[GA]^CG[CT]C
KasI 18 GGCGCC G^GCGCC
KspI 33 CCGCGG CCGC^GG
MaeII 113 ACGT A^CGT
MboI 700 GATC ^GATC
MfeI 926 CAATTG C^AATTG
MunI 926 CAATTG C^AATTG
NaeI 64 GCCGGC GCC^GGC
NarI 18 GGCGCC GG^CGCC
NdeI 908 CATATG CA^TATG
NdeII 700 GATC ^GATC
NgoMIV 64 GCCGGC G^CCGGC
NheI 55 GCTAGC G^CTAGC
PflMI 248 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1025 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 644 CTGCAG CTGCA^G
PvuII 482 CAGCTG CAG^CTG
RcaI 1384 TCATGA T^CATGA
SacII 33 CCGCGG CCGC^GG
Sau3AI 700 GATC ^GATC
SfoI 18 GGCGCC GGC^GCC
SmlI 730 CTTGAG C^T[CT][GA]AG
Tsp45I 1065 GTCAC ^GT[GC]AC
Van91I 248 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1473 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 34 CGCG CG^CG
135 CGCG
AfaI 245 GTAC GT^AC
746 GTAC
AlwNI 404 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
1315 CAGAAACTG
AvaII 931 GGTCC G^G[AT]CC
1026 GGTCC
BalI 183 TGGCCA TGG^CCA
1322 TGGCCA
BmyI 138 GGGCAC G[AGT]GC[ACT]^C
1455 GAGCCC
BsiEI 30 CGGCCG CG[GA][CT]^CG
128 CGGCCG
Bsp1286I 138 GGGCAC G[AGT]GC[ACT]^C
1455 GAGCCC
BstF5I 860 CATCC GGATG(2/0)
609 GGATG
BstUI 34 CGCG CG^CG
135 CGCG
EagI 30 CGGCCG C^GGCCG
128 CGGCCG
EclXI 30 CGGCCG C^GGCCG
128 CGGCCG
Eco52I 30 CGGCCG C^GGCCG
128 CGGCCG
FokI 860 CATCC GGATG(9/13)
609 GGATG
HaeII 18 GGCGCC [GA]GCGC^[CT]
37 GGCGCT
HgaI 133 GACGC GACGC(5/10)
208 GACGC
HindIII 178 AAGCTT A^AGCTT
1510 AAGCTT
HphI 213 TCACC GGTGA(8/7)
968 TCACC
MluNI 183 TGGCCA TGG^CCA
1322 TGGCCA
MscI 183 TGGCCA TGG^CCA
1322 TGGCCA
MvnI 34 CGCG CG^CG
135 CGCG
PleI 722 GACTC GAGTC(4/5)
1114 GACTC
RsaI 245 GTAC GT^AC
746 GTAC
SfcI 644 CTGCAG C^T[GA][CT]AG
835 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AcsI AluI ApoI BanI
BbvI BcnI BfaI BsaJI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BstNI Cac8I CfoI Cfr13I DdeI
EaeI EcoRII FauI Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MaeI MaeIII MboII MnlI
MseI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV Sau96I ScrFI
SfaNI TfiI Tru9I TseI Tsp509I
TspRI