################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaWI BseAI BseRI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HpaI KpnI Ksp632I MamI MflI MluI MroI MslI NcoI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AcyI 18 GGCGCC G[GA]^CG[CT]C Asp700I 1473 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanII 1455 GAGCCC G[GA]GC[CT]^C BbeI 18 GGCGCC GGCGC^C BbsI 195 GAAGAC GAAGAC(2/6) BbvCI 147 CCTCAGC CCTCAGC(-5/-2) BpuAI 195 GAAGAC GAAGAC(2/6) BsaHI 18 GGCGCC G[GA]^CG[CT]C BsaI 1197 GAGACC GGTCTC(1/5) BsgI 160 GTGCAG GTGCAG(16/14) BspHI 1384 TCATGA T^CATGA BspMI 1251 GCAGGT ACCTGC(4/8) BsrFI 64 GCCGGC [GA]^CCGG[CT] BssSI 1202 CACGAG CACGAG(-5/-1) BstAPI 637 GCAAAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1209 GGTTACC G^GT[ACGT]ACC BstPI 1209 GGTTACC G^GT[ACGT]ACC Cfr10I 64 GCCGGC [GA]^CCGG[CT] DpnII 700 GATC ^GATC DraI 1256 TTTAAA TTT^AAA DraII 1025 GGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 33 CCGCGG C^C[GA][CT]GG EcoNI 726 CCTGCTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1025 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoO65I 1209 GGTTACC G^GT[ACGT]ACC Hin1I 18 GGCGCC G[GA]^CG[CT]C KasI 18 GGCGCC G^GCGCC KspI 33 CCGCGG CCGC^GG MaeII 113 ACGT A^CGT MboI 700 GATC ^GATC MfeI 926 CAATTG C^AATTG MunI 926 CAATTG C^AATTG NaeI 64 GCCGGC GCC^GGC NarI 18 GGCGCC GG^CGCC NdeI 908 CATATG CA^TATG NdeII 700 GATC ^GATC NgoMIV 64 GCCGGC G^CCGGC NheI 55 GCTAGC G^CTAGC PflMI 248 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1025 GGGTCCT [GA]G^G[AT]CC[CT] PstI 644 CTGCAG CTGCA^G PvuII 482 CAGCTG CAG^CTG RcaI 1384 TCATGA T^CATGA SacII 33 CCGCGG CCGC^GG Sau3AI 700 GATC ^GATC SfoI 18 GGCGCC GGC^GCC SmlI 730 CTTGAG C^T[CT][GA]AG Tsp45I 1065 GTCAC ^GT[GC]AC Van91I 248 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 1473 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 34 CGCG CG^CG 135 CGCG AfaI 245 GTAC GT^AC 746 GTAC AlwNI 404 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG 1315 CAGAAACTG AvaII 931 GGTCC G^G[AT]CC 1026 GGTCC BalI 183 TGGCCA TGG^CCA 1322 TGGCCA BmyI 138 GGGCAC G[AGT]GC[ACT]^C 1455 GAGCCC BsiEI 30 CGGCCG CG[GA][CT]^CG 128 CGGCCG Bsp1286I 138 GGGCAC G[AGT]GC[ACT]^C 1455 GAGCCC BstF5I 860 CATCC GGATG(2/0) 609 GGATG BstUI 34 CGCG CG^CG 135 CGCG EagI 30 CGGCCG C^GGCCG 128 CGGCCG EclXI 30 CGGCCG C^GGCCG 128 CGGCCG Eco52I 30 CGGCCG C^GGCCG 128 CGGCCG FokI 860 CATCC GGATG(9/13) 609 GGATG HaeII 18 GGCGCC [GA]GCGC^[CT] 37 GGCGCT HgaI 133 GACGC GACGC(5/10) 208 GACGC HindIII 178 AAGCTT A^AGCTT 1510 AAGCTT HphI 213 TCACC GGTGA(8/7) 968 TCACC MluNI 183 TGGCCA TGG^CCA 1322 TGGCCA MscI 183 TGGCCA TGG^CCA 1322 TGGCCA MvnI 34 CGCG CG^CG 135 CGCG PleI 722 GACTC GAGTC(4/5) 1114 GACTC RsaI 245 GTAC GT^AC 746 GTAC SfcI 644 CTGCAG C^T[GA][CT]AG 835 CTACAG ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AcsI AluI ApoI BanI BbvI BcnI BfaI BsaJI BsiYI BslI BsmAI BsmFI BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI EaeI EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeI MaeIII MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp509I TspRI