##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflIII AgeI AhdI AlwNI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BbrPI BbvCI BcgI BciVI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BseRI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CelII ClaI
CpoI DraI DrdI Eam1105I Eco47III
EcoNI EcoO65I EcoRV EcoT14I EcoT22I
FauI FseI HpaI KpnI KspI
MaeII MamI MfeI MluI MroI
MunI MvnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcyI 182 GGCGCC G[GA]^CG[CT]C
AflII 682 CTTAAG C^TTAAG
Alw44I 149 GTGCAC G^TGCAC
ApaLI 149 GTGCAC G^TGCAC
AviII 1226 TGCGCA TGC^GCA
BalI 1342 TGGCCA TGG^CCA
BamHI 362 GGATCC G^GATCC
BbeI 182 GGCGCC GGCGC^C
BbsI 627 GTCTTC GAAGAC(2/6)
BfrI 682 CTTAAG C^TTAAG
BpuAI 627 GTCTTC GAAGAC(2/6)
BsaHI 182 GGCGCC G[GA]^CG[CT]C
BsaI 972 GGTCTC GGTCTC(1/5)
BsgI 712 CTGCAC GTGCAG(16/14)
BsiEI 71 CGGCCG CG[GA][CT]^CG
BsmAI 973 GTCTC GTCTC(1/5)
BspMI 768 GCAGGT ACCTGC(4/8)
BstYI 362 GGATCC [GA]^GATC[CT]
DraIII 1320 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 71 CGGCCG C^GGCCG
EarI 594 GAAGAG CTCTTC(1/4)
EclXI 71 CGGCCG C^GGCCG
Eco52I 71 CGGCCG C^GGCCG
FspI 1226 TGCGCA TGC^GCA
HaeII 182 GGCGCC [GA]GCGC^[CT]
HgaI 378 GACGC GACGC(5/10)
Hin1I 182 GGCGCC G[GA]^CG[CT]C
HindIII 1106 AAGCTT A^AGCTT
KasI 182 GGCGCC G^GCGCC
Ksp632I 594 GAAGAG CTCTTC(1/4)
MflI 362 GGATCC [GA]^GATC[CT]
MluNI 1342 TGGCCA TGG^CCA
MscI 1342 TGGCCA TGG^CCA
MslI 389 CATGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 182 GGCGCC GG^CGCC
SacI 639 GAGCTC GAGCT^C
ScaI 722 AGTACT AGT^ACT
SfoI 182 GGCGCC GGC^GCC
SmlI 682 CTTAAG C^T[CT][GA]AG
XhoII 362 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 1156 GAATTC [GA]^AATT[CT]
1353 GAATTC
AfaI 723 GTAC GT^AC
1233 GTAC
AocI 732 CCTGAGG CC^T[ACGT]AGG
967 CCTTAGG
ApoI 1156 GAATTC [GA]^AATT[CT]
1353 GAATTC
AvaI 30 CTCGGG C^[CT]CG[GA]G
864 CTCGGG
BanI 182 GGCGCC G^G[CT][GA]CC
850 GGCACC
BcnI 15 CCCGG CC^[GC]GG
1150 CCGGG
BpmI 510 CTCCAG CTGGAG(16/14)
254 CTGGAG
BsmI 112 GCATTC GAATGC(1/-1)
894 GCATTC
BsoBI 30 CTCGGG C^[CT]CG[GA]G
864 CTCGGG
BsrFI 530 GCCGGT [GA]^CCGG[CT]
801 GCCGGT
BstNI 95 CCTGG CC^[AT]GG
253 CCTGG
BstXI 343 CCATCCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
543 CCAGTTGACTGG
Bsu36I 732 CCTGAGG CC^T[ACGT]AGG
967 CCTTAGG
CfoI 183 GCGC GCG^C
1227 GCGC
Cfr10I 530 GCCGGT [GA]^CCGG[CT]
801 GCCGGT
EaeI 71 CGGCCG [CT]^GGCC[GA]
1342 TGGCCA
Eco81I 732 CCTGAGG CC^T[ACGT]AGG
967 CCTTAGG
EcoRI 1156 GAATTC G^AATTC
1353 GAATTC
EcoRII 95 CCTGG ^CC[AT]GG
253 CCTGG
HhaI 183 GCGC GCG^C
1227 GCGC
HinP1I 183 GCGC G^CGC
1227 GCGC
HincII 501 GTTGAC GT[CT]^[GA]AC
546 GTTGAC
HindII 501 GTTGAC GT[CT]^[GA]AC
546 GTTGAC
MspA1I 513 CAGCTG C[AC]G^C[GT]G
743 CAGCTG
MvaI 95 CCTGG CC^[AT]GG
253 CCTGG
NciI 15 CCCGG CC^[GC]GG
1150 CCGGG
PleI 504 GACTC GAGTC(4/5)
625 GAGTC
PpuMI 334 GGGACCT [GA]G^G[AT]CC[CT]
757 GGGTCCT
PvuII 513 CAGCTG CAG^CTG
743 CAGCTG
RsaI 723 GTAC GT^AC
1233 GTAC
SfaNI 76 GCATC GCATC(5/9)
708 GCATC
SfcI 808 CTGTAG C^T[GA][CT]AG
977 CTGTAG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BanII BbvI BclI BfaI BmyI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I Cac8I
Cfr13I DdeI DpnII DraII DsaI
EcoO109I FbaI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MspI MwoI NdeII NlaIII
NlaIV NspI Sau3AI Sau96I ScrFI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I