################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflIII AgeI AhdI AlwNI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BbrPI BbvCI BcgI BciVI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BseRI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII ClaI CpoI DraI DrdI Eam1105I Eco47III EcoNI EcoO65I EcoRV EcoT14I EcoT22I FauI FseI HpaI KpnI KspI MaeII MamI MfeI MluI MroI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcyI 182 GGCGCC G[GA]^CG[CT]C AflII 682 CTTAAG C^TTAAG Alw44I 149 GTGCAC G^TGCAC ApaLI 149 GTGCAC G^TGCAC AviII 1226 TGCGCA TGC^GCA BalI 1342 TGGCCA TGG^CCA BamHI 362 GGATCC G^GATCC BbeI 182 GGCGCC GGCGC^C BbsI 627 GTCTTC GAAGAC(2/6) BfrI 682 CTTAAG C^TTAAG BpuAI 627 GTCTTC GAAGAC(2/6) BsaHI 182 GGCGCC G[GA]^CG[CT]C BsaI 972 GGTCTC GGTCTC(1/5) BsgI 712 CTGCAC GTGCAG(16/14) BsiEI 71 CGGCCG CG[GA][CT]^CG BsmAI 973 GTCTC GTCTC(1/5) BspMI 768 GCAGGT ACCTGC(4/8) BstYI 362 GGATCC [GA]^GATC[CT] DraIII 1320 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 71 CGGCCG C^GGCCG EarI 594 GAAGAG CTCTTC(1/4) EclXI 71 CGGCCG C^GGCCG Eco52I 71 CGGCCG C^GGCCG FspI 1226 TGCGCA TGC^GCA HaeII 182 GGCGCC [GA]GCGC^[CT] HgaI 378 GACGC GACGC(5/10) Hin1I 182 GGCGCC G[GA]^CG[CT]C HindIII 1106 AAGCTT A^AGCTT KasI 182 GGCGCC G^GCGCC Ksp632I 594 GAAGAG CTCTTC(1/4) MflI 362 GGATCC [GA]^GATC[CT] MluNI 1342 TGGCCA TGG^CCA MscI 1342 TGGCCA TGG^CCA MslI 389 CATGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 182 GGCGCC GG^CGCC SacI 639 GAGCTC GAGCT^C ScaI 722 AGTACT AGT^ACT SfoI 182 GGCGCC GGC^GCC SmlI 682 CTTAAG C^T[CT][GA]AG XhoII 362 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AcsI 1156 GAATTC [GA]^AATT[CT] 1353 GAATTC AfaI 723 GTAC GT^AC 1233 GTAC AocI 732 CCTGAGG CC^T[ACGT]AGG 967 CCTTAGG ApoI 1156 GAATTC [GA]^AATT[CT] 1353 GAATTC AvaI 30 CTCGGG C^[CT]CG[GA]G 864 CTCGGG BanI 182 GGCGCC G^G[CT][GA]CC 850 GGCACC BcnI 15 CCCGG CC^[GC]GG 1150 CCGGG BpmI 510 CTCCAG CTGGAG(16/14) 254 CTGGAG BsmI 112 GCATTC GAATGC(1/-1) 894 GCATTC BsoBI 30 CTCGGG C^[CT]CG[GA]G 864 CTCGGG BsrFI 530 GCCGGT [GA]^CCGG[CT] 801 GCCGGT BstNI 95 CCTGG CC^[AT]GG 253 CCTGG BstXI 343 CCATCCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 543 CCAGTTGACTGG Bsu36I 732 CCTGAGG CC^T[ACGT]AGG 967 CCTTAGG CfoI 183 GCGC GCG^C 1227 GCGC Cfr10I 530 GCCGGT [GA]^CCGG[CT] 801 GCCGGT EaeI 71 CGGCCG [CT]^GGCC[GA] 1342 TGGCCA Eco81I 732 CCTGAGG CC^T[ACGT]AGG 967 CCTTAGG EcoRI 1156 GAATTC G^AATTC 1353 GAATTC EcoRII 95 CCTGG ^CC[AT]GG 253 CCTGG HhaI 183 GCGC GCG^C 1227 GCGC HinP1I 183 GCGC G^CGC 1227 GCGC HincII 501 GTTGAC GT[CT]^[GA]AC 546 GTTGAC HindII 501 GTTGAC GT[CT]^[GA]AC 546 GTTGAC MspA1I 513 CAGCTG C[AC]G^C[GT]G 743 CAGCTG MvaI 95 CCTGG CC^[AT]GG 253 CCTGG NciI 15 CCCGG CC^[GC]GG 1150 CCGGG PleI 504 GACTC GAGTC(4/5) 625 GAGTC PpuMI 334 GGGACCT [GA]G^G[AT]CC[CT] 757 GGGTCCT PvuII 513 CAGCTG CAG^CTG 743 CAGCTG RsaI 723 GTAC GT^AC 1233 GTAC SfaNI 76 GCATC GCATC(5/9) 708 GCATC SfcI 808 CTGTAG C^T[GA][CT]AG 977 CTGTAG ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AluI AlwI AspHI AvaII BanII BbvI BclI BfaI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I Cac8I Cfr13I DdeI DpnII DraII DsaI EcoO109I FbaI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MspI MwoI NdeII NlaIII NlaIV NspI Sau3AI Sau96I ScrFI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I